{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T16:12:44Z","timestamp":1782922364237,"version":"3.54.5"},"reference-count":37,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2024-0393_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Congenital adrenal hyperplasia (CAH) is a rare, inherited disorder of adrenal steroid synthesis. In many countries it is part of the neonatal screening program enabling early diagnosis and treatment. In case of an abnormal neonatal screening result or when other differences of sexual development (DSD) are suspected, measurement of serum steroid hormones using liquid chromatography coupled to mass spectrometry (LC-MS\/MS) is needed for further diagnosis. However, reliable age- and sex-specific reference intervals (RIs) for serum steroid hormones during the neonatal period are missing. We therefore aimed to establish LC-MS\/MS based RIs for serum steroid hormones in neonates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2024-0393_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Serum was obtained from healthy term neonates at two time points: 130 samples at day 3\u20138 (\n                      <jats:italic>T1<\/jats:italic>\n                      , time of the neonatal screening) and 126 samples at day 13\u201315 (\n                      <jats:italic>T2,<\/jats:italic>\n                      \u00a0two weeks old). Concentrations of cortisol, cortisone, corticosterone, 11-deoxycortisol, 21-deoxycortisol, 11-deoxycorticosterone, testosterone, androstenedione, and 17-hydroxyprogesterone (17-OHP) were measured using LC-MS\/MS.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2024-0393_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      RIs (in nmol\/L) were established for\n                      <jats:italic>T1<\/jats:italic>\n                      and\n                      <jats:italic>T2<\/jats:italic>\n                      : cortisone (19.3\u2013215;18.0\u2013212), cortisol (10.0\u2013407;8.4\u2013446), corticosterone (&lt;31;&lt;50), 11-deoxycortisol (0.73\u20134.6;0.70\u20133.6), 17-OHP (&lt;4.9;&lt;5.1), androstenedione (0.3\u20131.8;0.3\u20132.7), 11-deoxycorticosterone (&lt;0.2;&lt;0.2), and 21-deoxycortisol (&lt;1;&lt;1), respectively. Testosterone differed between boys and girls: RIs at\n                      <jats:italic>T1<\/jats:italic>\n                      and\n                      <jats:italic>T2<\/jats:italic>\n                      for boys were 0.27\u20134.3 and 0.63\u201313.9, and for girls&lt;0.30 and &lt;0.47, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2024-0393_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We established LC-MS\/MS based RIs for cortisol, cortisone, corticosterone, 11-deoxycortisol, 21-deoxycortisol, 11-deoxycorticosterone, testosterone, androstenedione, and 17-OHP in neonates in the first and second week of life.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2024-0393","type":"journal-article","created":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T22:50:42Z","timestamp":1729291842000},"page":"805-811","source":"Crossref","is-referenced-by-count":8,"title":["Neonatal reference intervals for serum steroid hormone concentrations measured by LC-MS\/MS"],"prefix":"10.1515","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3482-7240","authenticated-orcid":false,"given":"Anouk","family":"Olthof","sequence":"first","affiliation":[{"name":"Endocrine Laboratory, Department of Laboratory Medicine , Amsterdam UMC, Location University of Amsterdam and Vrije Universiteit Amsterdam , Amsterdam , The Netherlands"},{"name":"Amsterdam Gastroenterology Endocrinology Metabolism , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3787-0811","authenticated-orcid":false,"given":"Jolanda C.","family":"Naafs","sequence":"additional","affiliation":[{"name":"Amsterdam Gastroenterology Endocrinology Metabolism , Amsterdam , The Netherlands"},{"name":"Department of Pediatric Endocrinology , Emma Children\u2019s Hospital, Amsterdam UMC, Location University of Amsterdam , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0318-5541","authenticated-orcid":false,"given":"Nitash","family":"Zwaveling-Soonawala","sequence":"additional","affiliation":[{"name":"Amsterdam Gastroenterology Endocrinology Metabolism , Amsterdam , The Netherlands"},{"name":"Department of Pediatric Endocrinology , Emma Children\u2019s Hospital, Amsterdam UMC, Location University of Amsterdam , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2772-4538","authenticated-orcid":false,"given":"Charlotte A.","family":"Heinen","sequence":"additional","affiliation":[{"name":"Amsterdam Gastroenterology Endocrinology Metabolism , Amsterdam , The Netherlands"},{"name":"Department of Pediatric Endocrinology , Emma Children\u2019s Hospital, Amsterdam UMC, Location University of Amsterdam , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8996-0993","authenticated-orcid":false,"given":"Sabine E.","family":"Hannema","sequence":"additional","affiliation":[{"name":"Amsterdam Gastroenterology Endocrinology Metabolism , Amsterdam , The Netherlands"},{"name":"Department of Pediatric Endocrinology , Emma Children\u2019s Hospital, Amsterdam UMC, Location University of Amsterdam , Amsterdam , The Netherlands"},{"name":"Department of Pediatric Endocrinology , Amsterdam UMC, Location Vrije Universiteit Amsterdam , Amsterdam , The Netherlands"},{"name":"Amsterdam Reproduction and Development , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1161-0301","authenticated-orcid":false,"given":"Jacquelien J.","family":"Hillebrand","sequence":"additional","affiliation":[{"name":"Endocrine Laboratory, Department of Laboratory Medicine , Amsterdam UMC, Location University of Amsterdam and Vrije Universiteit Amsterdam , Amsterdam , The Netherlands"},{"name":"Amsterdam Gastroenterology Endocrinology Metabolism , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4994-2918","authenticated-orcid":false,"given":"Anita","family":"Boelen","sequence":"additional","affiliation":[{"name":"Endocrine Laboratory, Department of Laboratory Medicine , Amsterdam UMC, Location University of Amsterdam and Vrije Universiteit Amsterdam , Amsterdam , The Netherlands"},{"name":"Amsterdam Gastroenterology Endocrinology Metabolism , Amsterdam , The Netherlands"},{"name":"Amsterdam Reproduction and Development , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9677-7441","authenticated-orcid":false,"given":"Paul A.S.","family":"van Trotsenburg","sequence":"additional","affiliation":[{"name":"Amsterdam Gastroenterology Endocrinology Metabolism , Amsterdam , The Netherlands"},{"name":"Department of Pediatric Endocrinology , Emma Children\u2019s Hospital, Amsterdam UMC, Location University of Amsterdam , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6712-9955","authenticated-orcid":false,"given":"Annemieke C.","family":"Heijboer","sequence":"additional","affiliation":[{"name":"Endocrine Laboratory, Department of Laboratory Medicine , Amsterdam UMC, Location University of Amsterdam and Vrije Universiteit Amsterdam , Amsterdam , The Netherlands"},{"name":"Amsterdam Gastroenterology 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