{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:08:48Z","timestamp":1787022528730,"version":"build-2736575974"},"reference-count":18,"publisher":"Walter de Gruyter GmbH","issue":"2","funder":[{"name":"Agence Nationale pour la Recherche","award":["NECCIN"],"award-info":[{"award-number":["NECCIN"]}]},{"DOI":"10.13039\/100013465","name":"French Muscular Dystrophy Association","doi-asserted-by":"publisher","award":["grant#23593"],"award-info":[{"award-number":["grant#23593"]}],"id":[{"id":"10.13039\/100013465","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2024-0510_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Autoimmune nodopathy (AN) is a life-threatening peripheral neuropathy mediated by four autoantibodies targeting axoglial cell adhesion molecules at the nodes of\u00a0Ranvier: Neurofascin-155 (Nfasc155), PanNeurofascin (PanNfasc), Contactin-1 (CNTN1), and Contactin-associated protein 1 (CASPR1). Antibody detection is a strong biomarker for AN diagnosis and treatment monitoring. The aim of this study was to develop an immuno-dot assay (immuno-DOT) compatible with routine implementation in medical laboratories.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2024-0510_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This new approach was compared to standard techniques: indirect immunofluorescence assay, cell-based assay, and ELISA. Sensitivities (Se) and specificities (Sp) were calculated on a cohort composed of 58 patients diagnosed with AN, 50 seronegative patients with chronic inflammatory demyelinating polyradiculoneuropathy, 20 healthy controls, 30 patients with Guillain-Barr\u00e9 syndrome, 20 with monoclonal gammopathy and 20 with Charcot-Marie-Tooth disease. The patients were diagnosed with AN based on compatible electro-clinical arguments and at least two positive standard techniques.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2024-0510_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Immuno-DOT sensitivities and specificities were Se=91\u202f%, Sp=97\u202f% for anti-Nfasc155; Se=80\u202f%, Sp=94\u202f% for anti-PanNfasc; Se=93\u202f%, Sp=98\u202f% for anti-CNTN1; and Se=87\u202f%, Sp=94\u202f% for anti-CASPR1. Immuno-DOT allowed the diagnosis within 3\u202fh and the accurate follow-up of the immune reactivity and isotype, and dot intensity correlated with antibody titers following treatments. A longitudinal study indicated that immuno-DOT yielded reliable results even after six months of storage at\u00a0\u221220\u202f\u00b0C.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2024-0510_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The diagnostic performance of immuno-DOT was satisfactory and compatible with routine implementation in medical laboratories.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2024-0510","type":"journal-article","created":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T23:10:46Z","timestamp":1718147446000},"page":"346-355","source":"Crossref","is-referenced-by-count":5,"title":["An immuno-DOT diagnostic assay for autoimmune nodopathy"],"prefix":"10.1515","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0223-4359","authenticated-orcid":false,"given":"Alexandre","family":"Jentzer","sequence":"first","affiliation":[{"name":"131795 Institut de G\u00e9nomique Fonctionnelle, Universit\u00e9 de Montpellier, CNRS, INSERM , Montpellier , France"},{"name":"Department of Immunology , Saint Eloi University Hospital Center, Montpellier University , Montpellier , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Taieb","sequence":"additional","affiliation":[{"name":"131795 Institut de G\u00e9nomique Fonctionnelle, Universit\u00e9 de Montpellier, CNRS, INSERM , Montpellier , France"},{"name":"Department of Neurology , Gui de Chauliac University Hospital Center , Montpellier , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00e9mie","family":"El Bechir","sequence":"additional","affiliation":[{"name":"131795 Institut de G\u00e9nomique Fonctionnelle, Universit\u00e9 de Montpellier, CNRS, INSERM , Montpellier , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Vincent","sequence":"additional","affiliation":[{"name":"Department of Immunology , Saint Eloi University Hospital Center, Montpellier University , Montpellier , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me Jo\u00ebl","family":"Devaux","sequence":"additional","affiliation":[{"name":"131795 Institut de G\u00e9nomique Fonctionnelle, Universit\u00e9 de Montpellier, CNRS, INSERM , Montpellier , France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2024,6,12]]},"reference":[{"key":"2024123004263022496_j_cclm-2024-0510_ref_001","doi-asserted-by":"crossref","unstructured":"Einheber, S, Zanazzi, G, Ching, W, Scherer, S, Milner, TA, Peles, E, et al.. 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