{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T20:07:23Z","timestamp":1766088443908,"version":"3.48.0"},"reference-count":41,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T00:00:00Z","timestamp":1744848000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2024-1478_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Cortisone is an inert precursor\/metabolite of the potent steroid hormone cortisol. Measurement of serum cortisone levels and the cortisol-cortisone ratio can be useful for the diagnosis of dysfunction in the regulation of cortisol levels (i.e.,\u00a0severe and subtle apparent mineralocorticoid excess, low-renin primary aldosteronism). Therefore, an isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC MS\/MS)-based candidate reference measurement procedure (RMP) to quantify cortisone in human serum\/plasma was developed and validated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2024-1478_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Quantitative nuclear magnetic resonance (qNMR) was utilized to assign absolute content (g\/g) and SI-traceability to reference materials used as primary calibrators. A supported liquid extraction sample preparation protocol as well as a two-dimensional heart-cut LC approach for LC-MS\/MS analysis were employed to mitigate matrix effects and prevent co-elution of interferences. Selectivity was determined by analyzing a matrix sample containing the analyte, the internal standard and six potential interferents. A post-column infusion experiment and a comparison of standard line slopes were performed to evaluate matrix effects. An extensive protocol over five days was applied to determine precision, accuracy and trueness. Measurement uncertainty (MU) was evaluated in compliance with current guidelines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2024-1478_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>This RMP is suitable for analyzing cortisone within the 0.0800\u2013120\u202fng\/mL (0.222\u2013333\u202fnmol\/L) range, demonstrating selectivity, sensitivity and matrix independence. Intermediate precision was \u22643.4\u202f%, repeatability was \u22642.9\u202f% across all concentration levels and relative mean bias ranged from \u22123.7 to 2.8\u202f% across all tested matrices and concentrations. Expanded MU (k=2) for target value assignment (n=6) ranged from 2.1 to 5.5\u202f%, irrespective of concentration or sample\u00a0type.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2024-1478_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This RMP allows for accurate and reproducible determination of cortisone in human serum and plasma. Implementation of this method supports routine assay standardization and patient sample measurement with confirmed traceability.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2024-1478","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T10:08:24Z","timestamp":1744798104000},"page":"70-82","source":"Crossref","is-referenced-by-count":1,"title":["An isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure for the quantification of cortisone in human serum and plasma"],"prefix":"10.1515","volume":"64","author":[{"given":"Myriam","family":"Ott","sequence":"first","affiliation":[{"name":"Roche Diagnostics GmbH , Penzberg , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neeraj","family":"Singh","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH , Penzberg , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Kubicova","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH , Penzberg , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friederike","family":"Bauland","sequence":"additional","affiliation":[{"name":"Chrestos Concept GmbH & Co. KG , Essen , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"K\u00f6ppl","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine , University Hospital Erlangen , Erlangen , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7197-3868","authenticated-orcid":false,"given":"Alexander","family":"Gaudl","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig Medical Center , Leipzig , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Geistanger","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH , Penzberg , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uta","family":"Ceglarek","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig Medical Center , Leipzig , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Rauh","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine , University Hospital Erlangen , Erlangen , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Geletneky","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH , Penzberg , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith","family":"Taibon","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH , Penzberg , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2025,4,17]]},"reference":[{"key":"2025121815555040714_j_cclm-2024-1478_ref_001","doi-asserted-by":"crossref","unstructured":"Ramamoorthy, S, Cidlowski, JA. 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