{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T05:40:09Z","timestamp":1756532409892,"version":"3.44.0"},"reference-count":29,"publisher":"Walter de Gruyter GmbH","issue":"10","funder":[{"name":"Noncommunicable Chronic Diseases-National Science and Technology Major Project","award":["2024ZD0533200"],"award-info":[{"award-number":["2024ZD0533200"]}]},{"name":"National Key Technologies R&D Program provided by Ministry of Science and Technology of the People\u2019s Republic of China","award":["2022YFC3602300\/2022YFC3602302"],"award-info":[{"award-number":["2022YFC3602300\/2022YFC3602302"]}]},{"name":"National High Level Hospital Clinical Research Funding","award":["2022-PUMCH-B-073"],"award-info":[{"award-number":["2022-PUMCH-B-073"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_cclm-2025-0022_abs_001\">\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Copeptin, a stable and simple-to-measure surrogate marker for arginine vasopressin (AVP), demonstrates excellent clinical values, particularly in diagnosing polyuria-polydipsia syndromes. However, conventional immunoassay methods are limited and lack comparability. Our aim was to establish a targeted liquid chromatography-tandem mass spectrometry (LC-MS\/MS) method for quantifying copeptin in human\u00a0serum.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2025-0022_abs_002\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Copeptin was extracted from serum using immunoprecipitation, digested with trypsin, and prepared using anion exchange solid-phase extraction before LC-MS\/MS detection. The analytical performance was validated in accordance with current guidelines. The method was compared to an immunofluorescent assay on the B.R.A.H.M.S platform.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2025-0022_abs_003\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The LC-MS\/MS method had a runtime of 8.5\u202fmin. The within-run precision ranged from 5.2 to 12.1\u202f%, and total coefficients of variation ranged from 8.1 to 13.5\u202f%. Copeptin quantitation showed linearity within the range of 5\u20131,000\u202fpg\/mL, with a limit of detection of 2.5\u202fpg\/mL. Recovery rates ranged from 95.2 to 103.1\u202f%, and no significant matrix effect was observed with internal standard correction. The LC-MS\/MS method had a good consistency with the immunoassay (r=0.926, slope=0.989). The reference interval for healthy individuals was 3.66\u201358.25\u202fpg\/mL.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2025-0022_abs_004\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We demonstrated the accuracy and reliability of this targeted LC-MS\/MS method for quantifying copeptin. This innovative application showed satisfactory precision, a wide linear range, and a low limit of detection. Clinical studies are anticipated to be conducted to assess diagnostic accuracy using this method.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/cclm-2025-0022","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T17:00:56Z","timestamp":1752080456000},"page":"1992-2001","source":"Crossref","is-referenced-by-count":0,"title":["A novel immunoprecipitation-based targeted liquid chromatography-tandem mass spectrometry analysis for accurate determination for copeptin in human serum"],"prefix":"10.1515","volume":"63","author":[{"given":"Danni","family":"Mu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine , 34732 Peking Union Medical College Hospital , Peking Union Medical College and Chinese Academy of Medical Science , Beijing , 100730 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , 34732 Peking Union Medical College Hospital , Peking Union Medical College and Chinese Academy of Medical Science , Beijing , 100730 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yichen","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , 34732 Peking Union Medical College Hospital , Peking Union Medical College and Chinese Academy of Medical Science , Beijing , 100730 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danchen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , 34732 Peking Union Medical College Hospital , Peking Union Medical College and Chinese Academy of Medical Science , Beijing , 100730 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutong","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory , Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences , Jinan , Shandong , P.R. 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