{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T16:01:55Z","timestamp":1766073715064,"version":"3.48.0"},"reference-count":29,"publisher":"Walter de Gruyter GmbH","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2025-0536_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>In 2023, Croatia implemented a screening program for familial hypercholesterolemia (FH) targeting children entering the first grade of primary school. The program is based on total cholesterol (TC) concentration measurements. This pilot study aimed to assess and compare TC results obtained using different analytical platforms during the program\u2019s initial phase (2023\u20132024).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2025-0536_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Retrospective data from laboratories across Croatia were analyzed. Results covered the following analyzers: Beckman Coulter AU (BC), Abbott Alinity\/Architect c (AA), Roche Cobas c (RC) and Siemens Atellica CH (SA). Statistical analyses included Kruskal\u2013Wallis and chi-square tests using the program\u2019s cut-off values. Bland\u2013Altman and Passing-Bablok analyses were used to assess differences in a direct comparison study of 40 samples. To reduce variability, TC values were also expressed as multiples of the median (MoM), calculated separately for each platform.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2025-0536_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The study included 17,694 children. Median TC values (IQR) differed significantly across platforms: BC (4.3 [3.8\u20134.7]\u00a0mmol\/L), AA (4.1 [3.7\u20134.5]), RC (4.1 [3.7\u20134.5]), and SA (4.0 [3.6\u20134.4]); p&lt;0.001. BC and SA results differed significantly from all other platforms, while no significant difference was found between RC and AA. The distribution of TC results also varied significantly (p&lt;0.001). In the comparison study, all platforms showed statistically significant differences (p&lt;0.001). In contrast, MoM values showed no statistically significant differences between analyzers (p=0.106) or in distribution when applying various MoM-based cut-offs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2025-0536_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Although variations in TC measurements across platforms are small, they are statistically significant and affect FH classification. MoM normalization may support harmonization in future screening protocols.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2025-0536","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T08:30:01Z","timestamp":1758097801000},"page":"215-224","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of total cholesterol results measured in the initial period of the Croatian screening program for familial hypercholesterolemia: a pilot study"],"prefix":"10.1515","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1937-8578","authenticated-orcid":false,"given":"Gordana","family":"Juri\u010di\u0107","sequence":"first","affiliation":[{"name":"Department of Laboratory Diagnostics , Pula General Hospital , Pula , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2633-3669","authenticated-orcid":false,"given":"Antonija","family":"Perovi\u0107","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Laboratory , Glavi\u0107 Health Care Institution , Dubrovnik , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7521-6285","authenticated-orcid":false,"given":"Jasminka","family":"Matica","sequence":"additional","affiliation":[{"name":"Medical-Biochemical Laboratory, Primary Health Centre , Primorje-Gorski Kotar County , Rijeka , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kornelija","family":"Perkovi\u0107-Radojkovi\u0107","sequence":"additional","affiliation":[{"name":"Medical-Biochemical Laboratory , Health Centre Zagreb East , Zagreb , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9554-4278","authenticated-orcid":false,"given":"Vesna","family":"Horvat","sequence":"additional","affiliation":[{"name":"Medical-Biochemical Laboratory Vesna Horvat , Osijek , Croatia"},{"name":"Department of Medical Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine Osijek , Osijek , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3792-4384","authenticated-orcid":false,"given":"Dario","family":"Mandi\u0107","sequence":"additional","affiliation":[{"name":"Medical-Biochemical Laboratory, Health Center for Osijek-Baranya County and Department of Laboratory Medicine and Pharmacy, Faculty of Medicine Osijek , Osijek , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamara","family":"Dev\u010di\u0107-Ljubi\u0107","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Laboratory and Transfusion\u00a0Medicine , General Hospital \u201eDr.An\u0111elko Vi\u0161i\u0107\u201c Bjelovar , Bjelovar , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zdravka","family":"Doljanin","sequence":"additional","affiliation":[{"name":"Medical-Biochemical Laboratory, Health Center of Split-Dalmatia County , Split , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irena","family":"U\u017eovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics , Zadar General Hospital , Zadar , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarina","family":"Jedrej\u010di\u0107","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Laboratory , Istrian Health Care Institution , Pazin , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonja","family":"Podolar","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Laboratory , General Hospital \u201cDr. Tomislav Bardek\u201d , Koprivnica , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nena","family":"Peran","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics , General Hospital of \u0160ibenik-Knin County , \u0160ibenik , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u017deljka","family":"Severinac","sequence":"additional","affiliation":[{"name":"Medical-Biochemical Laboratory Dubravka Ja\u0161ek , Zagreb , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ines","family":"Kelava","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics , General and Veteran Hospital \u201eCroatian Pride\u201c Knin , Knin , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivanka","family":"Maldini","sequence":"additional","affiliation":[{"name":"Medical-Biochemical Laboratory Ivanka Maldini , Dubrovnik , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marijan","family":"Or\u0161uli\u0107","sequence":"additional","affiliation":[{"name":"IN2 D.O.O , Zagreb , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kre\u0161imir","family":"Kule\u0161","sequence":"additional","affiliation":[{"name":"IN2 D.O.O , Zagreb , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"key":"2025121815555024108_j_cclm-2025-0536_ref_001","doi-asserted-by":"crossref","unstructured":"McGowan, MP, Hosseini Dehkordi, SH, Moriarty, PM, Duell, PB. 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