{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T11:55:33Z","timestamp":1782561333269,"version":"3.54.5"},"reference-count":23,"publisher":"Walter de Gruyter GmbH","issue":"11","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00c3\u00a5det","doi-asserted-by":"publisher","award":["2019-00795"],"award-info":[{"award-number":["2019-00795"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016413","name":"Mats Paulssons Stiftelse","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016413","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2025-0539_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Frequent blood sampling in vulnerable patient groups, such as prematurely born infants, can lead to significant blood loss and increased transfusion needs. Current pre-analytical technology requires comparably large blood volumes and leads to discarding of cells. This study investigates a device prototype enabling in-line sampling where cell-reduced plasma for clinical chemistry analyses is generated through acoustophoresis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2025-0539_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood samples were collected from healthy adult donors in lithium-heparin tubes without gel. Plasma separated via acoustophoresis was compared with centrifuged plasma (2000\u202fg\u00a0\u00d7\u00a010\u202fmin) for cell counts (n=14), cell-free hemoglobin (n=21), and 12 routine clinical chemistry analyte tests (n=21). Wilcoxon signed-rank tests and Bland Altman analysis were used for statistical comparison.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2025-0539_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Both acoustophoresis (AF) and centrifugation (CEN) generated cell-reduced plasma with&lt;0.01\u202f% of cells remaining after separation. However, compared to CEN plasma, more cells (median count per\u00a0\u03bcL 642 vs. 205, p&lt;0.01) and platelets (median count per\u00a0\u03bcL 20,477 vs. 1,537, p&lt;0.0001) remained in AF plasma. Cell-free hemoglobin (fHb) in AF plasma samples (range 0.0\u20130.2\u202fg\/L) was lower (p&lt;0.01) than in CEN plasma samples (range 0.1\u20130.3\u202fg\/L). Statistically significant relative mean differences in test results ranging from 0.84\u202f% (95\u202f% CI 0.48\u20131.19) for sodium to 10.50\u202f% (95\u202f% CI 5.02\u201315.99) for AST were\u00a0found.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2025-0539_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This proof-of-concept study demonstrates that acoustophoresis has the potential to produce sufficiently cell-free plasma for several commonly performed clinical chemistry analyses. Further studies should assess pathological samples, platelet activation, and improve the design for more efficient removal of platelets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2025-0539","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T04:57:07Z","timestamp":1751605027000},"page":"2218-2225","source":"Crossref","is-referenced-by-count":3,"title":["Acoustophoresis-based blood sampling and plasma separation for potentially minimizing sampling-related blood loss"],"prefix":"10.1515","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2508-1455","authenticated-orcid":false,"given":"Amal","family":"Nath","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering , 5193 Lund University , Lund , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2562-080X","authenticated-orcid":false,"given":"Sara Marie","family":"Larsson","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry , Halland Hospitals , Halmstad , Sweden"},{"name":"Department of Clinical Sciences, Pediatrics , Lund University, Sk\u00e5ne University Hospital , Lund , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Lenshof","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering , 5193 Lund University , Lund , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering , 5193 Lund University , Lund , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Baasch","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering , 5193 Lund University , Lund , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Pediatrics , Lund University, Sk\u00e5ne University Hospital , Lund , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Gram","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Pediatrics , Lund University, Sk\u00e5ne University Hospital , Lund , Sweden"},{"name":"Department of Biomedical Science, Faculty of Health and Society , Malm\u00f6 University , Malm\u00f6 , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Ley","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Pediatrics , Lund University, Sk\u00e5ne University Hospital , Lund , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Laurell","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering , 5193 Lund University , Lund , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"key":"2025103114402209303_j_cclm-2025-0539_ref_001","doi-asserted-by":"crossref","unstructured":"Ames, AC, Bamford, E. 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