{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:18:23Z","timestamp":1788779903758,"version":"build-2803163510"},"reference-count":13,"publisher":"Walter de Gruyter GmbH","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,25]]},"abstract":"Abstract<\/jats:title>\n \n Objectives<\/jats:title>\n \u03b1-Thalassaemia screening is crucial for identifying carriers at risk of having offspring with haemoglobin (Hb) Bart\u2019s hydrops fetalis syndrome. This study evaluated the performance of two potential screening methods: the \u03b1-thalassaemia early eluting peak (\u03b1EEP) identified by high-performance liquid chromatography (HPLC) and an immunochromatographic strip test (ICT), focusing on stability and potential interferences.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Ninety-two peripheral blood samples were used. Thirty were for assessment of \u03b1EEP and ICT stability and interference by icterus\/lipaemia, and saline washing for interference removal. Diagnostic performance of \u03b1EEP and ICT were evaluated in 40 samples with glycated Hb (P2)\u00a0\u22656.0\u202f% and 22 with Hb F\u22652.0\u202f% on HPLC, using \u03b1-globin genotyping as the gold standard.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Both \u03b1EEP and ICT results remained stable for 14\u00a0days. Neither test was significantly affected by icterus or lipaemia, though 3 of 20 ICT results (15\u202f%) showed discordance after saline washing. Elevated HbA\n 1c<\/jats:sub>\n and Hb F altered early eluting peak patterns but did not affect \u03b1EEP interpretation. For detecting\u00a0--\n SEA<\/jats:sup>\n mutation, \u03b1EEP showed 100\u202f% sensitivity and 100\u202f% specificity when P2\u22656.0\u202f% or Hb F\u22652.0\u202f%, while ICT a low specificity (45\u202f%) when Hb F\u22652.0\u202f%.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n \u03b1EEP showed reliable performance in detecting \u03b1\n 0<\/jats:sup>\n -thalassaemia with\u00a0--\n SEA<\/jats:sup>\n mutation in all conditions tested, while ICT showed low specificity when Hb F\u22652.0\u202f%. These findings support \u03b1EEP as a reliable test for routine clinical laboratory use, while cautions should be made for ICT in case of elevated Hb F levels.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1515\/cclm-2025-0754","type":"journal-article","created":{"date-parts":[[2025,8,16]],"date-time":"2025-08-16T12:56:16Z","timestamp":1755348976000},"page":"2549-2557","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of stability and potential interference on the \u03b1-thalassaemia early eluting peak and immunochromatographic strip test for \u03b1-thalassaemia\u00a0--\n SEA<\/sup>\n carrier screening"],"prefix":"10.1515","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6454-5729","authenticated-orcid":false,"given":"Wing Kit","family":"Lam","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology , 36658 Tuen Mun Hospital , Hong Kong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina Pui Ying","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology , 36658 Tuen Mun Hospital , Hong Kong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winnie Yim Fong","family":"Law","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology , 36658 Tuen Mun Hospital , Hong Kong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsz Fung","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology , 36658 Tuen Mun Hospital , Hong Kong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darcy Lok Han","family":"Too","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology , 36658 Tuen Mun Hospital , Hong Kong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lok Nga","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology , 36658 Tuen Mun Hospital , Hong Kong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anskar Yu Hung","family":"Leung","sequence":"additional","affiliation":[{"name":"Department of Medicine, Li Ka Shing Faculty of Medicine , The University of Hong Kong , Hong Kong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sze Fai","family":"Yip","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology , 36658 Tuen Mun Hospital , Hong Kong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,8,18]]},"reference":[{"key":"2025110712102421147_j_cclm-2025-0754_ref_001","unstructured":"Amid, A, Lal, A, Coates, TD, Fucharoen, S. 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