{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:50:40Z","timestamp":1792788640566,"version":"4.5.0"},"reference-count":74,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:00:00Z","timestamp":1760918400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Luzhou Medical Association Grant Project","award":["2025-YXXM-106"],"award-info":[{"award-number":["2025-YXXM-106"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>With the advent of flow cytometry and dedicated fluorescent dyes in the 1980s, automated reticulocyte enumeration has effectively superseded conventional manual microscopy, dramatically boosting throughput and precision while, through metrics including the immature reticulocyte fraction (IRF) and reticulocyte production index (RPI), delivering previously unattainable diagnostic resolution for anemia phenotyping, longitudinal tracking of marrow erythropoiesis, and evaluation of functional iron deficiency. Yet appreciable heterogeneity in analytical principles, reagent chemistry, and calibration frameworks persists across platforms, constraining result comparability and uniform interpretation across systems. The International Council for Standardization in Haematology (ICSH) is advancing commutable reference materials built on stable cell lines or synthetic microspheres, together with globally harmonized reference intervals, to achieve interoperable performance among instruments. Concurrently, the capital expense and servicing requirements of high-end fully automated analyzers continue to be a major impediment to clinical access to automated reticulocyte testing. This narrative review focuses on the \u201cstandardization and clinical accessibility of automated reticulocyte counting\u201d, systematically elaborating the core technical principles and characteristics of mainstream instruments, analyzing advances and challenges in standardization, and envisioning its application prospects in global clinical practice.<\/jats:p>","DOI":"10.1515\/cclm-2025-1032","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T07:56:28Z","timestamp":1760687788000},"page":"269-279","source":"Crossref","is-referenced-by-count":2,"title":["Automated reticulocyte counting: advances, standardization challenges, and clinical accessibility"],"prefix":"10.1515","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5539-8960","authenticated-orcid":false,"given":"Hongyin","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory , Zigong First People\u2019s Hospital , Zigong City , Sichuan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1802-3794","authenticated-orcid":false,"given":"Yaxin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Transfusion , Beijing Anzhen Nanchong Hospital of Capital Medical University & Nanchong Central Hospital , Nanchong City , Sichuan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruijian","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology , People\u2019s Hospital of Naxi District , Luzhou City , Sichuan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunyan","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory , People\u2019s Hospital of Naxi District , Luzhou City , Sichuan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaming","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory , Luzhou Mental Hospital , Luzhou City , Sichuan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianji","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory , Luzhou Mental Hospital , Luzhou City , Sichuan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1181-525X","authenticated-orcid":false,"given":"Yonggang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory , People\u2019s Hospital of Naxi District , Luzhou City , Sichuan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,10,20]]},"reference":[{"key":"2026031918102549235_j_cclm-2025-1032_ref_001","unstructured":"World Health Organization, Health Laboratory Technology and Blood Safety Unit, International Committee for Standardization in Haematology Expert Panel in Cytometry. 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