{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:29:48Z","timestamp":1786696188697,"version":"build-2736575974"},"reference-count":25,"publisher":"Walter de Gruyter GmbH","issue":"6","license":[{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2025-1523_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Serum protein electrophoresis is one of the keystone investigations for screening for monoclonal proteins and assessing the serum protein profile. The newly released Helena V8ultra capillary electrophoresis system has been evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2025-1523_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In total, 164 serum samples were assessed on the Helena V8 UltraCE system and compared with the Sebia Capillarys\u2122 instrument. Abnormalities suggestive of monoclonal proteins were confirmed by immunosubtraction. Imprecision studies intervals were determined. Special attention was paid to complement C3 polymorphism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2025-1523_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The imprecision of the Helena V8ultra was inferior or equal from 0.73\u202f% (albumin) to 4.32\u202f% (alpha\n                      <jats:sub>2<\/jats:sub>\n                      globulin). The mean bias of Helena V8ultraCE vs. Sebia Capillarys was about\u00a0\u22120.225\u202f% for albumin;\u00a0\u22120.791\u202f% for alpha\n                      <jats:sub>1<\/jats:sub>\n                      globulins; 1.353\u202f% for alpha\n                      <jats:sub>2<\/jats:sub>\n                      globulins;\u00a0\u22122.317\u202f% for beta globulins; 0.066\u202f% for gamma globulins. Among the 6 samples with monoclonal proteins confirmed by immunofixation, all were seen on both methods, with only 1 discordant result. The theoretical plate number (albumin fraction) was 2,371\u00a0\u00b1\u00a0718 (Helena V8UltraCE) vs. 445\u00a0\u00b1\u00a0115 (Sebia Capillarys 2). The high resolution also allows to distinguish Complement C3 phenotypes. CV values for the various fractions were low, ranging from 0.47\u202f% (albumin) to 0.89\u202f% (gamma globulins). IgG, IgA and IgM M-proteins could be detected in the electropherogram with an excellent sensitivity (&lt;0.1\u202fg\/L).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2025-1523_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our evaluation confirms the good analytical performance of the Helena V8 analyzer as a suitable alternative to the Sebia Capillarys instrument. The high resolution allows detailed analysis of individual protein fractions which is an excellent basis for studying microheterogeneity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2025-1523","type":"journal-article","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T16:55:47Z","timestamp":1767372947000},"page":"1399-1403","source":"Crossref","is-referenced-by-count":1,"title":["Validation study of the Helena V8 UltraCE capillary elecrophoresis analyser"],"prefix":"10.1515","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5702-6792","authenticated-orcid":false,"given":"Joris R.","family":"Delanghe","sequence":"first","affiliation":[{"name":"Labo Maenhout , Waregem , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maaike","family":"Godefroid","sequence":"additional","affiliation":[{"name":"Labo Maenhout , Waregem , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Maenhout","sequence":"additional","affiliation":[{"name":"Labo Maenhout , Waregem , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"2026081408005184086_j_cclm-2025-1523_ref_001","doi-asserted-by":"crossref","unstructured":"Morrison, T, Booth, RA, Hauff, K, Berardi, P, Visram, A. Laboratory assessment of multiple myeloma. Adv Clin Chem 2019;89:1\u201358. https:\/\/doi.org\/10.1016\/bs.acc.2018.12.001.","DOI":"10.1016\/bs.acc.2018.12.001"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_002","doi-asserted-by":"crossref","unstructured":"Booth, RA, McCudden, CR, Balion, CM, Blasutig, IM, Bouhtiauy, I, Rodriguez-Capote, K, et al.. Candidate recommendations for protein electrophoresis reporting from the Canadian society of clinical chemists monoclonal gammopathy working group. Clin Biochem 2018;51:10\u201320. https:\/\/doi.org\/10.1016\/j.clinbiochem.2017.10.013.","DOI":"10.1016\/j.clinbiochem.2017.10.013"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_003","doi-asserted-by":"crossref","unstructured":"Hage, DS. An overview of CE in clinical analysis. Methods Mol Biol 2019;1972:3\u201311. https:\/\/doi.org\/10.1007\/978-1-4939-9213-3_1.","DOI":"10.1007\/978-1-4939-9213-3_1"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_004","doi-asserted-by":"crossref","unstructured":"De Witte, E, Speeckaert, MM, Van De Moortel, L, Lecocq, E, Delanghe, J. Human complement factor 3 polymorphism determination by capillary electrophoresis of serum. Electrophoresis 2012;33:440\u20134. https:\/\/doi.org\/10.1002\/elps.201100451.","DOI":"10.1002\/elps.201100451"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_005","doi-asserted-by":"crossref","unstructured":"Lippi, G, Battistelli, L, Vernocchi, A, Mussap, M. Analytical evaluation of the novel Helena V8 capillary electrophoresis system. J\u00a0Med Biochem 2013;32:245\u20139. https:\/\/doi.org\/10.2478\/jomb-2013-0020.","DOI":"10.2478\/jomb-2013-0020"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_006","doi-asserted-by":"crossref","unstructured":"Poisson, J, Fedoriw, Y, Henderson, MP, Hainsworth, S, Tucker, K, Uddin, Z, et al.. Performance evaluation of the Helena V8 capillary electrophoresis system. Clin Biochem 2012;45:697\u20139. https:\/\/doi.org\/10.1016\/j.clinbiochem.2012.03.018.","DOI":"10.1016\/j.clinbiochem.2012.03.018"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_007","doi-asserted-by":"crossref","unstructured":"McCudden, CR, Mathews, SP, Hainsworth, SA, Chapman, JF, Hammett-Stabler, CA, Willis, MS, et al.. Performance comparison of capillary and agarose gel electrophoresis for the identification and characterization of monoclonal immunoglobulins. Am J Clin Pathol 2008;129:451\u20138. https:\/\/doi.org\/10.1309\/6KT8N49BRNVVVBT1.","DOI":"10.1309\/6KT8N49BRNVVVBT1"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_008","doi-asserted-by":"crossref","unstructured":"Favresse, J, Yolande, L, Gras, J. Evaluation of a capillary electrophoresis system for the separation of proteins. J\u00a0Appl Lab Med 2021;6:1611\u20137. https:\/\/doi.org\/10.1093\/jalm\/jfab044.","DOI":"10.1093\/jalm\/jfab044"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_009","doi-asserted-by":"crossref","unstructured":"Delanghe, J, Speeckaert, R, Speeckaert, M. Complement C3 and its polymorphism: biological and clinical consequences. Pathology 2014;46:1\u201310. https:\/\/doi.org\/10.1097\/pat.0000000000000042.","DOI":"10.1097\/PAT.0000000000000042"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_010","doi-asserted-by":"crossref","unstructured":"Vercauteren, KOA, Lambrecht, S, Delanghe, J. Pre-analytical classical and alternative complement pathway activity loss. Biochem Med 2019;29:030701, https:\/\/doi.org\/10.11613\/bm.2019.030701.","DOI":"10.11613\/BM.2019.030701"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_011","doi-asserted-by":"crossref","unstructured":"Zhang, J, Li, S, Hu, S, Yu, J, Xiang, Y. Association between genetic variation of complement C3 and the susceptibility to advanced age-related macular degeneration: a meta-analysis. BMC Ophthalmol 2018;18:274. https:\/\/doi.org\/10.1186\/s12886-018-0945-5.","DOI":"10.1186\/s12886-018-0945-5"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_012","doi-asserted-by":"crossref","unstructured":"Bossuyt, X. Separation of serum proteins by automated capillary zone electrophoresis. Clin Chem Lab Med 2003;41:762\u201372. https:\/\/doi.org\/10.1515\/cclm.2003.116.","DOI":"10.1515\/CCLM.2003.116"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_013","doi-asserted-by":"crossref","unstructured":"Yang, Z, Harrison, K, Park, YA, Chaffin, CH, Thigpen, B, Easley, PL, et al.. Performance of the Sebia CAPILLARYS 2 for detection and immunotyping of serum monoclonal Am J Clin Pathol 2007;128:293\u20139. https:\/\/doi.org\/10.1309\/1L3CG8GK6F8VYNYH.","DOI":"10.1309\/1L3CG8GK6F8VYNYH"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_014","doi-asserted-by":"crossref","unstructured":"Carter, RHJ, Gupta, P, Tyler, C, Micklitsch, C, Arrode-Bruses, G, Snyder, J. Evaluation of the analytical performance of three major immunoglobulins assays on the atellica CI analyzer. Clin Chem 2024;70: hvae 106\u2013697 https:\/\/doi.org\/10.1093\/clinchem\/hvae106.697.","DOI":"10.1093\/clinchem\/hvae106.697"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_015","doi-asserted-by":"crossref","unstructured":"Delanghe, S, Moerman, A, Pletinck, A, Van, B, Schepers, E, Glorieux, G, et al.. Quantification of carbamylated albumin in serum based on capillary electrophoresis. Electrophoresis 2017;38:2135\u201340. https:\/\/doi.org\/10.1002\/elps.201700068.","DOI":"10.1002\/elps.201700068"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_016","doi-asserted-by":"crossref","unstructured":"Favresse, J, Delanghe, J. High-resolution capillary electrophoresis for the determination of carbamylated albumin. Clin Chem Lab Med 2021;60:229\u201334. https:\/\/doi.org\/10.1515\/cclm-2021-0926.","DOI":"10.1515\/cclm-2021-0926"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_017","doi-asserted-by":"crossref","unstructured":"Chartier, C, Boularan, AM, Dupuy, AM, Badiou, S, Bargnoux, AS, Cognot, C, et al.. Evaluation of two automated capillary electrophoresis systems for human serum protein analysis. Clin Biochem 2011;44:1473\u20139. https:\/\/doi.org\/10.1016\/j.clinbiochem.2011.05.022.","DOI":"10.1016\/j.clinbiochem.2011.05.022"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_018","doi-asserted-by":"crossref","unstructured":"Ric\u00f3s, C, Alvarez, V, Cava, F, Garcia-Lano, Hern\u00e1ndez, A, Jim\u00e9nez, CV, et al.. Current databases on biological variation, pros, cons and progress. Scand J Clin Lab Invest;199:491\u2013500. https:\/\/doi.org\/10.1080\/00365519950185229.","DOI":"10.1080\/00365519950185229"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_019","doi-asserted-by":"crossref","unstructured":"Berg\u00f3n, E, Miranda, I, Miravalles, E. Linearity and detection limit in the measurement of serum M-protein with the capillary zone electrophoresis system capillarys. Clin Chem Lab Med 2005;43:721\u20133. https:\/\/doi.org\/10.1515\/CCLM.2005.122.","DOI":"10.1515\/CCLM.2005.122"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_020","doi-asserted-by":"crossref","unstructured":"Jacobs, JFM, Turner, KA, Graziani, MS, Frinack, JL, Ettore, MW, Tate, JR, et al.. An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. Part II: limit of detection and follow-up of patients with small M-proteins. Clin Chem Lab Med 2020;58:547\u201359. https:\/\/doi.org\/10.1515\/cclm-2019-1105.","DOI":"10.1515\/cclm-2019-1105"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_021","doi-asserted-by":"crossref","unstructured":"Seaux, L, Van Houcke, S, Dumoulin, E, Fiers, T, Lecocq, E, Delanghe, J. Dual wavelength recording, a simple algorithm to eliminate interferences due to UV absorbing substances in capillary electrophoresis. Electrophoresis 2014;35:2248\u201352. https:\/\/doi.org\/10.1002\/elps.201400259.","DOI":"10.1002\/elps.201400259"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_022","doi-asserted-by":"crossref","unstructured":"Bossuyt, X. Interferences in clinical capillary zone electrophoresis of serum proteins. Electrophoresis 2004;25:1485\u20137. https:\/\/doi.org\/10.1002\/elps.200305820.","DOI":"10.1002\/elps.200305820"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_023","doi-asserted-by":"crossref","unstructured":"Vermeersch, P, Mari\u00ebn, G, Bossuyt, X. Pseudoparaproteinemia related to iomeprol administration after angiocardiography: detection in the beta fraction by capillary zone electrophoresis clin. Chem 2006;52:2312\u20133. https:\/\/doi.org\/10.1373\/clinchem.2006.078584.","DOI":"10.1373\/clinchem.2006.078584"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_024","doi-asserted-by":"crossref","unstructured":"Brouwers, A, Mari\u00ebn, G, Bossuyt, X. Interference of sulfamethoxazole in capillarys electrophoresis. Clin Chem Lab Med 2006;44:910\u20131. https:\/\/doi.org\/10.1515\/cclm.2006.161.","DOI":"10.1515\/CCLM.2006.161"},{"key":"2026081408005184086_j_cclm-2025-1523_ref_025","doi-asserted-by":"crossref","unstructured":"Siede, D, M\u00f6ller, H, Siede, WH, Regeniter, A. Effect of ampicillin-sulbactam on clinical capillary zone electrophoresis of serum proteins. Clin Chem Lab Med 2008;46:1468\u20139. https:\/\/doi.org\/10.1515\/cclm.2008.281.","DOI":"10.1515\/CCLM.2008.281"}],"container-title":["Clinical Chemistry and Laboratory Medicine (CCLM)"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/cclm-2025-1523\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/cclm-2025-1523\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:02:20Z","timestamp":1786694540000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/cclm-2025-1523\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,5]]},"references-count":25,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,4,13]]},"published-print":{"date-parts":[[2026,5,26]]}},"alternative-id":["10.1515\/cclm-2025-1523"],"URL":"https:\/\/doi.org\/10.1515\/cclm-2025-1523","relation":{},"ISSN":["1434-6621","1437-4331"],"issn-type":[{"value":"1434-6621","type":"print"},{"value":"1437-4331","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,5]]}}}