{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:01:47Z","timestamp":1790420507520,"version":"4.1.0"},"reference-count":33,"publisher":"Walter de Gruyter GmbH","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2026-0962_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Humoral parameters like immunoglobulins, complement components, and free light chains (FLC) are vital for diagnosing immune and hematological disorders. To minimize pre-analytical errors and reduce turnaround time, this study evaluated the 7-day stability of 11 analytes (IgG, IgG subclasses 2\u20134, IgA, IgM, C1 esterase inhibitor, C3c, C4, and FLC Kappa\/Lambda) to determine if manual aliquoting can be eliminated in favor of direct primary tube sampling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2026-0962_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Blood from 27 healthy volunteers was collected into lithium heparin (LH) and LH-gel separator (LH-GS) tubes. Baseline measurements (Day 1) from aliquoted samples were compared against samples stored at 2\u20138\u202f\u00b0C and re-tested on Days 3 and 7 using the Optilite\n                      <jats:sup>\u00ae<\/jats:sup>\n                      analyzer. Stability was defined by the percent difference (%PD) against total error (TEa) limits.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2026-0962_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>IgG (and subclasses), IgA, IgM, and C4 remained highly stable through Day 7 in both tube types. FLCs (Kappa\/Lambda) showed a progressive negative bias but stayed within acceptable limits. Conversely, C3c exhibited significant positive bias, exceeding stability limits by Day 7 (%PD 10.39 in LH); it was only stable up to Day 3. No interference from the gel separator was detected.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2026-0962_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Aliquoting can be safely omitted for most humoral parameters, allowing for direct primary tube sampling and streamlined workflows. While FLCs remain reliable for 7\u00a0days, C3c requires a strict 3-day window. Both LH and LH-GS tubes are suitable, supporting laboratory optimization and ISO 15189 compliance while maintaining analytical quality.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2026-0962","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:55:36Z","timestamp":1790420136000},"source":"Crossref","is-referenced-by-count":0,"title":["Refrigerated storage stability of 11 humoral parameters in two different venous blood collection tubes over a 7-day period"],"prefix":"10.1515","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1243-9145","authenticated-orcid":false,"given":"Stijn","family":"De Koninck","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine , University Hospital of Brussels , Brussels , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joren","family":"Hemelings","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , University Hospital of Brussels , Brussels , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simke","family":"Demeester","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , University Hospital of Brussels , Brussels , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"2026092610553396880_j_cclm-2026-0962_ref_001","unstructured":"Janeway, CA, Travers, P, Walport, M, Shlomchik, MJ. 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