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The presence of one of these risk alleles confers a 2- to 4-fold increase in the risk of developing Crohn's disease, and the presence of two mutant alleles increases the risk over 20-fold.<\/jats:p><jats:p>To facilitate the analysis of these polymorphisms, we developed three LightCycler assays to detect the missense mutations Arg702Trp and Gly908Arg and the frameshift mutation Leu100fsinsC in the<\/jats:p>","DOI":"10.1515\/cclm.2004.084","type":"journal-article","created":{"date-parts":[[2004,8,25]],"date-time":"2004-08-25T15:11:12Z","timestamp":1093446672000},"source":"Crossref","is-referenced-by-count":7,"title":["Detection of the Arg702Trp, Gly908Arg and Leu1007fsinsC polymorphisms of the NOD2\/CARD15 gene by real-time PCR with melting curve 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