{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T11:49:56Z","timestamp":1695642596205},"reference-count":36,"publisher":"Walter de Gruyter GmbH","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Anti-proteinase 3 antineutrophil cytoplasmic antibodies (PR3-ANCA) and anti-myeloperoxidase antibodies (MPO-ANCA) are considered important serological markers for several forms of idiopathic systemic vasculitis. The aim of the study was to verify the analytical and clinical performance of a new automated enzyme fluoroimmunoassay, the EliA system, for PR3-ANCA and MPO-ANCA detection.<\/jats:p><jats:p>For this purpose the sera of 52 consecutive well-defined patients with a clinical diagnosis of Wegener\u2019s granulomatosis (WG) (n = 29) or microscopic polyangiitis (MPA) (n = 23), and 70 controls suffering from connective tissue disease (25 systemic lupus erythematosus, 25 Sj\u00f6gren\u2019s syndrome and 20 rheumatoid arthritis) were tested for PR3-ANCA and MPO-ANCA with the EliA assay (Pharmacia Diagnostics, Freiburg, Germany). For comparison purposes, the same sera were also tested by indirect immunofluorescence, another direct immunometric assay (Varelisa, Pharmacia Diagnostics) and a capture PR3-ANCA (Wieslab AB, Lund, Sweden) method.<\/jats:p><jats:p>Both the EliA PR3-ANCA and MPO-ANCA assays showed between- and within-assay precision of &lt; 10%. The dilution test gave straight lines (r<\/jats:p><jats:p>There was a high positive correlation between the EliA and Varelisa methods for quantitative detection of MPO-ANCA levels (r<\/jats:p><jats:p>In conclusion, the EliA MPO-ANCA and PR3-ANCA methods provide good diagnostic accuracy and excellent analytical accuracy, which, in association with the practicality of the automated EliA system, make this method a useful tool for the diagnosis of ANCA-associated vasculitides.<\/jats:p>","DOI":"10.1515\/cclm.2004.236","type":"journal-article","created":{"date-parts":[[2004,10,18]],"date-time":"2004-10-18T17:38:06Z","timestamp":1098121086000},"source":"Crossref","is-referenced-by-count":7,"title":["Analytical and diagnostic accuracy of the EliA\u2122 automated enzyme fluoroimmunoassay for antineutrophil cytoplasmic autoantibody detection"],"prefix":"10.1515","volume":"42","author":[{"given":"Danilo","family":"Villalta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elio","family":"Tonutti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilina","family":"Tampoia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Bizzaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Papisch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato","family":"Tozzoli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Stella","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref321","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/S0025-6196(12)65300-2","article-title":"Anticytoplasmic autoantibodies in the diagnosis and follow up of Wegener s granulomatosis","volume":"64","author":"Specks","year":"1989","journal-title":"Mayo Clin Proc"},{"key":"ref181","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1002\/art.1780310302","article-title":"The American Rheumatism Association revised criteria for the classification of rheumatoid arthritis","volume":"31","author":"Arnett","year":"1987","journal-title":"Arthritis Rheum"},{"key":"ref261","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1093\/rheumatology\/41.11.1313","article-title":"A critical evaluation of commercial immunoassays for antineutrophil cytoplasmic antibodies directed against proteinase and myeloperoxidase in Wegener s granulomatosis and microscopic polyangiitis","volume":"41","author":"Csernok","year":"2002","journal-title":"Rheumatology"},{"key":"ref371","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1046\/j.1523-1755.2003.00821.x","article-title":"Serial ANCA titers : useful tool for prevention of relapses in ANCA - 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