{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T18:43:32Z","timestamp":1781203412504,"version":"3.54.1"},"reference-count":12,"publisher":"Walter de Gruyter GmbH","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neuroblastoma is the most common extracranial solid tumor in children. Abnormal secretion of catechol-amines in tissues and body fluids allows for the differential diagnosis of neuroblastoma from other neoplasms and its distinction from non-neoplastic inflammatory diseases. This is achieved by assaying homovanillic acid and 4-hydroxy-3-methoxymandelic acid, the catabolites of catecholamine metabolism. In the course of an evaluation of children with suspected neuroblastoma, homovanillic acid and 4-hydroxy-3-methoxymandelic acid were analyzed in urine samples by capillary gas chromatography with flame ionization detection after extraction and derivatization of these compounds as trimethylsilyl derivatives. In three urine samples a significant increase in biogenic amines was observed, but these results were not confirmed by thin-layer chromatography. Patient history revealed that these children had been treated with ibuprofen, an analgesic and anti-inflammatory drug. To verify how ibuprofen or its metabolites may have interfered with capillary gas chromatography with flame ionization detection, we analyzed the same samples by capillary gas chromatography-mass spectrometry. In urine samples from patients on the drug, the presence of a peak identified as the trimethylsilyl ester of hydroxyibuprofen, which had the same retention time as 4-hydroxy-3-methoxymandelic acid, was found to interfere with the capillary gas chromatography with flame ionization detection analysis of the metabolite. This interference must be taken into account during the laboratory diagnosis of neuroblastoma.<\/jats:p>","DOI":"10.1515\/cclm.2005.029","type":"journal-article","created":{"date-parts":[[2005,2,14]],"date-time":"2005-02-14T17:59:23Z","timestamp":1108403963000},"source":"Crossref","is-referenced-by-count":2,"title":["The secretion of ibuprofen metabolites interferes with the capillary chromatography of urinary homovanillic acid and 4-hydroxy-3-methoxymandelic acid in neuroblastoma diagnosis"],"prefix":"10.1515","volume":"43","author":[{"given":"Isabella","family":"Levreri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ubaldo","family":"Caruso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franca","family":"Deiana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Buoncompagni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"De Bernardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Marchese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Melioli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"ref91","first-page":"1","article-title":"Instrumental consideration in catecholamines analysis using liquid chroma - 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dose protocols adopted by the Italian Co - Operative Group for Neuroblastoma","volume":"21","author":"De Bernardi","year":"2003","journal-title":"J Clin Oncol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0305-7372(76)80005-9","article-title":"Neuroblastoma review of the literature and an examination of its enigmatic character","volume":"3","author":"Jaffe","year":"1971","journal-title":"Cancer Treat Rep"},{"key":"ref101","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0009-8981(84)90181-5","article-title":"Simultaneous determination of catecholamines and metanephrines in urine by HPLC with fluorimetric detection","volume":"137","author":"Abeling","year":"1984","journal-title":"Clin Chim Acta"},{"key":"ref131","first-page":"148","article-title":"Toxicological detection of ibuprofen and its metabolites in urine using gas chromatography - mass spectrometry MS )","volume":"49","author":"Maurer","year":"1994","journal-title":"Pharmazie"},{"key":"ref71","first-page":"696","article-title":"Simultaneous quantitative determination of the major phase I and II metabolites of Ibuprofen in biological fluid by high - 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