{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T16:03:12Z","timestamp":1698681792586},"reference-count":17,"publisher":"Walter de Gruyter GmbH","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>: The aim of this study was to evaluate the diagnostic value of different detection methods for cytomegalovirus (CMV) infection after renal transplantation and also to establish a system to monitor therapy for CMV infection.<\/jats:p><jats:p>: We retrospectively studied 1516 renal transplant recipients from June 1994 to December 2006. All patients were screened for CMV-DNA. A total of 1402 patients had received CMV-IgG\/IgM detection since June 1996 and 660 had received CMV antigen detection since June 2000.<\/jats:p><jats:p>: A total of 664 (43.8%) recipients developed CMV infection. The sensitivity, specificity and Youden index of the three methods, respectively, were 18.84%, 100% and 0.1884 for ELISA, 91.86%, 82.98% and 0.7484 for PCR, and 88.06%, 96.95% and 0.8501 for the CMV-pp65 antigenemia test. The sensitivity and specificity of the two combined detection methods (CMV-DNA and CMV-pp65) for post-operation CMV infection were 93.49% and 99.06%; the two detection methods had significant dependability (p&lt;0.05) in diagnosis of CMV infection and in evaluation of therapeutic effect of antiviral drugs.<\/jats:p><jats:p>: Only ELISA can be used as a screening index in order to distinguish whether the donors or recipients are infected with CMV or not. CMV-pp65 antigenemia can help guide clinical therapy for CMV infection. CMV-pp65 and CMV-PCR combined together provide a more effective method to monitor CMV infection and predict its outcome.<\/jats:p><jats:p>Clin Chem Lab Med 2009;47:177\u201381.<\/jats:p>","DOI":"10.1515\/cclm.2009.033","type":"journal-article","created":{"date-parts":[[2009,2,4]],"date-time":"2009-02-04T13:27:05Z","timestamp":1233754025000},"source":"Crossref","is-referenced-by-count":1,"title":["Methodology for monitoring cytomegalovirus infection after renal transplantation"],"prefix":"10.1515","volume":"47","author":[{"given":"Wujun","family":"Xue","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Puxun","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoming","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoming","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinshun","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heli","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/CMR.10.1.86","volume":"10","author":"Patel R","year":"1997","journal-title":"Clin Microbiol Rev"},{"key":"p_2","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1681\/ASN.V124848","volume":"12","author":"Brennan DC","year":"2001","journal-title":"J Am Soc Nephrol"},{"key":"p_3","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1111\/j.1365-2249.1990.tb06441.x","volume":"80","author":"Giessen M","year":"1990","journal-title":"Clin Exp Immunol"},{"key":"p_4","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.1128\/JCM.36.9.2681-2685.1998","volume":"36","author":"Tong CY","year":"1998","journal-title":"J Clin Microbiol"},{"key":"p_5","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1128\/JCM.30.7.1763-1767.1992","volume":"30","author":"Boland GJ","year":"1992","journal-title":"J Clin Microbiol"},{"key":"p_6","doi-asserted-by":"publisher","DOI":"10.1086\/515383"},{"key":"p_7","doi-asserted-by":"crossref","first-page":"3585","DOI":"10.1128\/JCM.36.12.3585-3589.1998","volume":"36","author":"Gerna G","year":"1998","journal-title":"J Clin Microbiol"},{"key":"p_8","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199910270-00011"},{"key":"p_9","doi-asserted-by":"publisher","DOI":"10.1086\/339329"},{"key":"p_10","doi-asserted-by":"publisher","DOI":"10.1159\/000190140"},{"key":"p_11","doi-asserted-by":"publisher","DOI":"10.1086\/507337"},{"key":"p_12","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2007.08.040"},{"key":"p_13","doi-asserted-by":"publisher","DOI":"10.1086\/324516"},{"key":"p_14","first-page":"49","volume":"57","author":"Orlic P","year":"2003","journal-title":"Acta Med Croatica"},{"key":"p_15","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199911150-00015"},{"key":"p_16","doi-asserted-by":"publisher","DOI":"10.1086\/315242"},{"key":"p_17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3062.2006.00171.x"}],"container-title":["Clinical Chemistry and Laboratory Medicine"],"original-title":[],"link":[{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/CCLM.2009.033\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T02:14:18Z","timestamp":1633054458000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/CCLM.2009.033\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,1]]},"references-count":17,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1515\/cclm.2009.033","relation":{},"ISSN":["1437-4331","1434-6621"],"issn-type":[{"value":"1437-4331","type":"electronic"},{"value":"1434-6621","type":"print"}],"subject":[],"published":{"date-parts":[[2009,1,1]]}}}