{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:29:56Z","timestamp":1762100996518},"reference-count":23,"publisher":"Walter de Gruyter GmbH","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Chemistry and Laboratory Medicine"],"published-print":{"date-parts":[[2010,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>\n                  <jats:bold>Background:<\/jats:bold> The free \u03b2 subunit of human chorionic gonadotropin (free \u03b2-hCG) is an important serum marker for biochemical screening.  Its weekly median value varies with ethnicity.  Most of the fluorometers for lanthanide chelates are designed for the detection of signals from europium (Eu<jats:sup>3+<\/jats:sup>) chelates only.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methods:<\/jats:bold> We developed a two-site, one-step assay using two monoclonal antibodies (MAbs) against free \u03b2 subunit and \u03b2 subunit with Eu<jats:sup>3+<\/jats:sup> chelates as labels.  Using the present assay, we evaluated 24,634 normal serum samples in Chinese pregnant women during 8\u201320\u00a0weeks of gestation.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results:<\/jats:bold> The detection limit using this assay was &lt;0.05 ng\/mL.  The within-run and between-run imprecision was &lt;6.0% and 7.0% using control material.  Free \u03b2-hCG concentrations measured using the current assay in 999 maternal serum samples correlated well with those obtained by samarium (Sm<jats:sup>3+<\/jats:sup>)-labeled DELFIA free hCG\u03b2 assay (r=0.987).  The medians for 8\u201320\u00a0weeks for maternal serum free \u03b2-hCG were higher in the women from mainland China compared to reports from other countries.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions:<\/jats:bold> The present assay is suitable for use in biochemical screening of women in mainland China.  Our study on  the median concentrations of free \u03b2-hCG will help establish reference values that are specific for ethnic populations from the Chinese mainland.  These will be useful for studying the importance of ethnic factors in biochemical screening.<\/jats:p>\n               <jats:p>Clin Chem Lab Med 2010;48:109\u201314.<\/jats:p>","DOI":"10.1515\/cclm.2010.005","type":"journal-article","created":{"date-parts":[[2009,11,30]],"date-time":"2009-11-30T09:08:30Z","timestamp":1259572110000},"page":"109-114","source":"Crossref","is-referenced-by-count":3,"title":["Determination of median levels of the free \u03b2 subunit of human chorionic gonadotropin in women from mainland China using a new time-resolved fluoroimmunoassay"],"prefix":"10.1515","volume":"48","author":[{"given":"Yong-Ping","family":"Tang","sequence":"first","affiliation":[{"name":"School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-Song","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai-Hua","family":"Yin","sequence":"additional","affiliation":[{"name":"Prenatal Diagnosis Center, Guangdong Women and Children Hospital and Health Institute, Guangzhou, Guangdong Province, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Wen","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Southern Medical University, Guangzhou, Guangdong Province, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng-Hui","family":"Yuan","sequence":"additional","affiliation":[{"name":"Guangzhou Darui Antibody Engineering and Technology Co., Ltd, Guangzhou, Guangdong Province, P.R. 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