{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:21:31Z","timestamp":1761862891898},"reference-count":15,"publisher":"Walter de Gruyter GmbH","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Chemistry and Laboratory Medicine"],"published-print":{"date-parts":[[2010,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>\n                  <jats:bold>Background:<\/jats:bold> Human \u03b2-defensins (hBDs) are small cationic, widely expressed proteins involved in innate immunity that exert strong bactericidal activity toward various pathogens. However, the role of hBDs in various diseases to which bacterial infection add severity, as it is in celiac disease (CD), is not yet clear. We analyzed the expression of the <jats:italic>hBD1<\/jats:italic>, <jats:italic>hBD2<\/jats:italic>, <jats:italic>hBD3<\/jats:italic> and <jats:italic>hBD4<\/jats:italic> genes in patients with CD during the active phase and after remission following a gluten-free diet to determine their role in development and relapse of CD.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methods:<\/jats:bold> We studied 21 unrelated adults with CD (confirmed by anti-thyroglobulin antibodies and intestinal biopsy); 14 were evaluated at diagnosis, before diet modification, and seven after 2\u00a0years of a gluten-free diet. Thirty-six unrelated adults served as controls. We analyzed the mRNA expression of <jats:italic>hBD1, 2, 3<\/jats:italic> and <jats:italic>4<\/jats:italic> on biopsy samples of duodenum obtained from all patients during endoscopy for diagnostic purposes. We used real-time polymerase chain reaction with TaqMan probes and obtained gene expression data using the delta-Ct method.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results:<\/jats:bold>\n                  <jats:italic> hBD1<\/jats:italic> mRNA was significantly lower in patients with active CD compared with patients on diet modification, whereas the mRNA levels of the other three defensins did not differ significantly between the two subgroups. Interestingly, the gluten-free diet restored only partially hBD1 expression as compared to a normal group of celiac-free subjects.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions:<\/jats:bold> Our data reinforce the evidence that <jats:italic>hBD1<\/jats:italic> expression is greatly reduced in the duodenum of patients with active CD. It also strengthens the concept that reduced activity of immune peptides may predispose individuals to bacterial proliferation that contributes to the pathogenesis of CD.<\/jats:p>\n               <jats:p>Clin Chem Lab Med 2010;48:489\u201392.<\/jats:p>","DOI":"10.1515\/cclm.2010.098","type":"journal-article","created":{"date-parts":[[2010,2,4]],"date-time":"2010-02-04T10:35:57Z","timestamp":1265279757000},"page":"489-492","source":"Crossref","is-referenced-by-count":7,"title":["Low expression of human \u03b2-defensin 1 in duodenum of celiac patients is partially restored by a gluten-free diet"],"prefix":"10.1515","volume":"48","author":[{"given":"Mariano","family":"Intrieri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Assunta","family":"Rinaldi","sequence":"additional","affiliation":[{"name":"CEINGE-Biotecnologie Avanzate, Scarl, Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Scudiero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni","family":"Autiero","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Clinica e Sperimentale, Universit\u00e0 degli Studi di Napoli Federico II, Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Castaldo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerardo","family":"Nardone","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Clinica e Sperimentale, Universit\u00e0 degli Studi di Napoli Federico II, Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2010,2,4]]},"reference":[{"key":"p_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2004.04158.x"},{"key":"p_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2003.07379.x"},{"key":"p_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2005.02.017"},{"key":"p_4","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5439.498"},{"key":"p_5","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2004.0124.x"},{"key":"p_6","doi-asserted-by":"publisher","DOI":"10.1038\/ncpgasthep0265"},{"key":"p_7","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5540-2"},{"key":"p_8","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2007.06.008"},{"key":"p_9","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-005-0657-1"},{"key":"p_10","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"p_11","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1096\/fj.00-0865fje","volume":"15","author":"Garcia JR","year":"2001","journal-title":"FASEB J"},{"key":"p_12","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-005-2575-z"},{"key":"p_13","doi-asserted-by":"publisher","DOI":"10.1038\/43088"},{"key":"p_14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2004.04157.x"},{"key":"p_15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2005.02.003"}],"container-title":["cclm"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/CCLM.2010.098\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/CCLM.2010.098\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T05:04:36Z","timestamp":1680843876000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/CCLM.2010.098\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2,4]]},"references-count":15,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2010,2,12]]},"published-print":{"date-parts":[[2010,4,1]]}},"alternative-id":["10.1515\/CCLM.2010.098"],"URL":"https:\/\/doi.org\/10.1515\/cclm.2010.098","relation":{},"ISSN":["1437-4331","1434-6621"],"issn-type":[{"value":"1437-4331","type":"electronic"},{"value":"1434-6621","type":"print"}],"subject":[],"published":{"date-parts":[[2010,2,4]]}}}