{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T08:11:35Z","timestamp":1767773495985},"reference-count":33,"publisher":"Walter de Gruyter GmbH","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Chemistry and Laboratory Medicine"],"published-print":{"date-parts":[[2010,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>\n                  <jats:bold>Background:<\/jats:bold> DNA piezoelectric biosensors have become a promising tool in molecular medicine since they do not require any label or staining. Here, a DNA piezoelectric biosensor based on a quartz crystal microbalance (QCM) was created to identify abnormal genes causing \u03b1-thalassemia 1 (SEA deletion).<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methods:<\/jats:bold> The functionalized gold electrode of the quartz crystal was coated with avidin and the biotinylated DNA probe was attached. The target gene causing \u03b1-thalassemia 1 was amplified and hybridized with the immobilized probe. DNA hybridization was indicated by changes in the quartz resonance frequencies. Diagnostic ability of the new \u03b1-thalassemia 1 biosensor was validated using both known and unknown blood samples. Specificity was tested using samples of \u03b2-thalassemia and \u03b1-thalassemia 2. Stability of the sensor was also evaluated.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results:<\/jats:bold> The new biosensor could clearly identify \u03b1-thalassemia 1 (SEA deletion), both carrier and disease states, from the normal genotype. Identification accuracy was compatible to the standard gel electrophoresis. It was specific only to \u03b1-thalassemia 1 since no cross reaction was found with \u03b2-thalassemia and \u03b1-thalassemia 2. The sensor could be kept at room temperature up to 6\u00a0months with consistent identification accuracy.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions:<\/jats:bold> The label free QCM based biosensor was successfully developed to diagnose an abnormal human globin gene causing \u03b1-thalassemia 1 (SEA deletion). Its accuracy, specificity and sensitivity were comparable to the standard method. Its stable diagnostic potency up to 6\u00a0months implied its field application in thalassemic control program.<\/jats:p>\n               <jats:p>Clin Chem Lab Med 2010;48:1247\u201354.<\/jats:p>","DOI":"10.1515\/cclm.2010.258","type":"journal-article","created":{"date-parts":[[2010,6,28]],"date-time":"2010-06-28T06:20:23Z","timestamp":1277706023000},"page":"1247-1254","source":"Crossref","is-referenced-by-count":13,"title":["Quartz crystal microbalance-based biosensor for the detection of \u03b1-thalassemia 1 (SEA deletion)"],"prefix":"10.1515","volume":"48","author":[{"given":"Sirinart","family":"Chomean","sequence":"first","affiliation":[{"name":"Faculty of Medical Technology, Department of Clinical Microscopy, Mahidol University, Nakhon Pathom, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiparat","family":"Potipitak","sequence":"additional","affiliation":[{"name":"Division of Quality Monitoring, National Laboratory Animal Center, Mahidol University, Nakhon Pathom, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chamras","family":"Promptmas","sequence":"additional","affiliation":[{"name":"Faculty of Medical Technology, Department of Clinical Chemistry, Mahidol University, Nakhon Pathom, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanida","family":"Ittarat","sequence":"additional","affiliation":[{"name":"Faculty of Medical Technology, Department of Clinical Microscopy, Mahidol University, Nakhon Pathom, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2010,6,28]]},"reference":[{"key":"p_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01337937"},{"key":"p_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2007.12.035"},{"key":"p_3","doi-asserted-by":"publisher","DOI":"10.1021\/ja051345q"},{"key":"p_4","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(03)00065-5"},{"key":"p_5","doi-asserted-by":"publisher","DOI":"10.1016\/S0734-9750(97)00003-7"},{"key":"p_6","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-5663(03)00087-3"},{"key":"p_7","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-5663(00)00092-0"},{"key":"p_8","first-page":"547","author":"Weatherall DJ","year":"2001","journal-title":"Williams Hematology. 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