{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T15:25:35Z","timestamp":1778167535364,"version":"3.51.4"},"reference-count":29,"publisher":"Walter de Gruyter GmbH","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Chemistry and Laboratory Medicine"],"published-print":{"date-parts":[[2011,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>\n                  <jats:bold>Background:<\/jats:bold> Daptomycin is a recently developed cyclic lipopeptide antibiotic active against most Gram-positive pathogens including vancomycin-resistant enterococci and methicillin-resistant <jats:italic>Staphylococcus aureus<\/jats:italic>. To optimize treatment efficacy and safety, especially in patients undergoing multiple drug regimens and\/or co-morbidities, a specific liquid chromatography-tandem mass spectrometry (LC-MS\/MS) method was developed for the quantification of daptomycin in plasma.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methods:<\/jats:bold> A C18 column was used for separation, with a mobile phase initially consisting of 0.1% formic acid, water, and acetonitrile (ACN) in a linear gradient from 20% to 70%. After protein precipitation with ACN, the clear upper layer was diluted in water:ACN (50:50, v\/v) before injection. Detection was performed using an electrospray ionization technique. MS\/MS transitions, monitored in the positive ion mode were <jats:italic>m\/z<\/jats:italic> 811.1 \u2192 <jats:italic>m\/z<\/jats:italic> 313.1 for daptomycin, and <jats:italic>m\/z<\/jats:italic> 609.4 \u2192 <jats:italic>m\/z<\/jats:italic> 194.9 for reserpine, used as internal standard.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results:<\/jats:bold> Elution of daptomycin and reserpine occurred at 4.5 and 3.9\u00a0min, respectively. The method was validated over a range of concentrations from 1\u00a0mg\/L to 120\u00a0mg\/L. The assay met recommended acceptance criteria: coefficients of variation were &lt;6.3% and &lt;7.4%, and accuracies were between \u20135.9% and +11.2% and between \u20133.5% and +3.7%, for intra- and inter-day validations, respectively.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions:<\/jats:bold> This method appears well-adapted to routine hospital practice for therapeutic drug monitoring of daptomycin considering its time of analysis, range of concentrations measured, precision and accuracy.<\/jats:p>","DOI":"10.1515\/cclm.2011.005","type":"journal-article","created":{"date-parts":[[2010,10,20]],"date-time":"2010-10-20T13:02:30Z","timestamp":1287579750000},"page":"69-75","source":"Crossref","is-referenced-by-count":45,"title":["Determination of daptomycin in human plasma by liquid chromatography-tandem mass spectrometry. Clinical application"],"prefix":"10.1515","volume":"49","author":[{"given":"Marie-Cl\u00e9mence","family":"Verdier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dani\u00e8le","family":"Bentu\u00e9-Ferrer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Tribut","sequence":"additional","affiliation":[{"name":"Biological Pharmacology and Toxicology, University Hospital, Rennes 1 University, Rennes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Collet","sequence":"additional","affiliation":[{"name":"Biological Pharmacology and Toxicology, University Hospital, Rennes 1 University, Rennes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthieu","family":"Revest","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Intensive Care, University Hospital, Rennes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Bellissant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2010,10,20]]},"reference":[{"key":"p_3","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh546"},{"key":"p_4","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.jinf.2007.05.180","volume":"55","author":"Enoch DA","year":"2007","journal-title":"Daptomycin. 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