{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,4,8]],"date-time":"2023-04-08T04:13:49Z","timestamp":1680927229781},"reference-count":14,"publisher":"Walter de Gruyter GmbH","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Chemistry and Laboratory Medicine"],"published-print":{"date-parts":[[2011,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>\n                  <jats:bold>Background:<\/jats:bold> The determination of polymerase chain reaction (PCR) amplification product sizes of the <jats:italic>Bcl-<\/jats:italic>2<jats:italic>\/IgH<\/jats:italic> fusion gene from follicular lymphoma (FL) provides evidence of clonal identity.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methods:<\/jats:bold> The present study describes detection of <jats:italic>Bcl-<\/jats:italic>2<jats:italic>\/IgH<\/jats:italic> fusion gene clonality utilizing a small, simple microcapillary electrophoretic chip combined with a real-time PCR method.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results:<\/jats:bold> The microcapillary electrophoretic chip system effectively detects size differences among the <jats:italic>Bcl-2\/IgH<\/jats:italic> fusion gene amplification products of FL from patient samples; something that is not possible using traditional gel electrophoresis. We also describe the potential of this system to utilize formalin-fixed, paraffin-embedded tissue samples sectioned on charged slides.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions:<\/jats:bold> The simple detection of <jats:italic>Bcl-2\/IgH<\/jats:italic> fusion gene clonality using a microcapillary electrophoretic chip provides reliable information for monitoring minimal residual disease of FL, and can be an effective tool for use in clinical laboratories.<\/jats:p>","DOI":"10.1515\/cclm.2011.141","type":"journal-article","created":{"date-parts":[[2011,2,11]],"date-time":"2011-02-11T10:46:18Z","timestamp":1297421178000},"page":"809-815","source":"Crossref","is-referenced-by-count":1,"title":["Identification of <i>Bcl-2\/IgH<\/i> fusion sequences using real-time PCR and chip-based microcapillary electrophoresis"],"prefix":"10.1515","volume":"49","author":[{"given":"Yoko","family":"Tabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Kawase","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazunori","family":"Miyake","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naotake","family":"Satoh","sequence":"additional","affiliation":[{"name":"Division of Clinical Laboratory, Juntendo Tokyo Koto Geriatric Medical Center, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nanae","family":"Aritaka","sequence":"additional","affiliation":[{"name":"Department of Hematology, Juntendo University School of Medicine, Tokyo, 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