{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T07:41:31Z","timestamp":1765525291456,"version":"3.48.0"},"reference-count":28,"publisher":"Walter de Gruyter GmbH","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_dx-2019-0006_s_999_w2aab3b7b3b1b6b1aab1c15b1Aa\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Liver abscess is the most common extraintestinal manifestation of\n                      <jats:italic>Entamoeba histolytica<\/jats:italic>\n                      . Clinical manifestations could appear after returning from an endemic area or several years after the exposure. The diagnosis usually requires microbiological confirmation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_dx-2019-0006_s_998_w2aab3b7b3b1b6b1aab1c15b2Aa\">\n                    <jats:title>Case presentation<\/jats:title>\n                    <jats:p>\n                      We present a case of a 55-year-old woman diagnosed with Crohn\u2019s disease treated with immunosuppressive drugs, who was admitted to the Emergency Service with liver parenchyma abscesses. Computed tomography (CT)-guided puncture showed pus, and both Gram staining and fresh parasite visualization were negative. Hepatic pus bacteriological culture was reported as negative and parasite multiplex polymerase chain reaction (PCR) was performed, being positive for\n                      <jats:italic>E. histolytica<\/jats:italic>\n                      . The same PCR was performed on blood, pleural fluid and stool samples, all of them being positive for\n                      <jats:italic>E. histolytica<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_dx-2019-0006_s_997_w2aab3b7b3b1b6b1aab1c15b3Aa\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Reviewing the clinical history of this patient, it was observed that parasite detection in three stool samples was negative 2 months before the current admission. Due to the lack of sensitivity of the microscopy techniques, we propose to routinely perform parasite detection in stools using molecular techniques, especially in immunocompromised patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/dx-2019-0006","type":"journal-article","created":{"date-parts":[[2019,6,29]],"date-time":"2019-06-29T05:06:16Z","timestamp":1561784776000},"page":"69-73","source":"Crossref","is-referenced-by-count":3,"title":["<i>Entamoeba histolytica<\/i>\n                    liver abscess case: could stool PCR avoid it?"],"prefix":"10.1515","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7373-8749","authenticated-orcid":false,"given":"Albert","family":"Bernet S\u00e1nchez","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology , Arnau de Vilanova University Hospital , Av Alcalde Rovira Roure 80 , 25198, Lleida , Spain , Phone: +84973705330"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alba","family":"Bell\u00e9s Bell\u00e9s","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology , Arnau de Vilanova University Hospital , Lleida , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesus","family":"Aramburu Arnuelos","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology , Arnau de Vilanova University Hospital , Lleida , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo","family":"Jover Sanz","sequence":"additional","affiliation":[{"name":"Nosocomial Infection Unit , Arnau de Vilanova University Hospital , Lleida , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Ses\u00e9 Abizanda","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology , Arnau de Vilanova University Hospital , Lleida , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Montserrat","family":"Vallverd\u00fa Vidal","sequence":"additional","affiliation":[{"name":"Intensive Care Unit , Arnau de Vilanova University Hospital , Lleida , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merc\u00e8","family":"Garc\u00eda Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology , Arnau de Vilanova University Hospital , Lleida , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2019,6,29]]},"reference":[{"key":"2025121207024372011_j_dx-2019-0006_ref_001_w2aab3b7b3b1b6b1ab2b1b1Aa","doi-asserted-by":"crossref","unstructured":"Lichtenstein A, Kondo AT, Visvesvara GS, Fernandez A, Paiva EF, Mauad T, et al. 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