{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T11:02:11Z","timestamp":1789902131952,"version":"4.0.1"},"reference-count":28,"publisher":"Walter de Gruyter GmbH","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Xiamen Natural Science Foundation","award":["3502Z202374077"],"award-info":[{"award-number":["3502Z202374077"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_dx-2026-0094_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The increasing demand for vitamin D measurement over the past few decades has prompted manufacturers to develop automated chemiluminescence immunoassay (CLIA), including the noncompetitive anti-immune complex assay (NACA) system for the measurement of small molecules. This study aims to evaluate the performance of the Snibe-NACA 25-OH Vitamin D assay for measuring total 25-Hydroxy Vitamin D assay [25(OH)D] levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_dx-2026-0094_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 200 serum samples were collected from a clinical laboratory for 25(OH)D assay to evaluate the performance of the Snibe-NACA 25-OH Vitamin D assay using a double-blind method. Liquid chromatography-tandem mass spectrometry (LC-MS\/MS) was used as a reference method and the results were compared with those obtained from four other chemiluminescence competitive 25(OH)D assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_dx-2026-0094_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The Snibe-NACA 25-OH Vitamin D assay demonstrated satisfactory analytical performance with optimal precision (repeatability CV: 1.13\u20132.75\u202f%;intra-laboratory CV: 1.59\u20132.58\u202f%) and relatively small bias (\u22123.84\u202f% mean relative bias compared with the LC-MS\/MS),excellent agreement with LC-MS\/MS, as evidenced by Passing-Bablok regression (R\n                      <jats:sup>2<\/jats:sup>\n                      : 0.9245, slope: 0.99, 95\u202f% CI: 0.96\u20131.03; intercept:\u00a0\u22121.24, 95\u202f% CI:\u00a0\u22123.49 to 0.65) and high correlation (Spearman\u2019s \u03c1=0.969; ICC=0.9584). Notably, the assay showed the highest concordance with LC-MS\/MS in the classification of vitamin D status, achieving an overall agreement of 87.0\u202f%, with particularly strong concordance in identifying deficiency (97.8\u202f%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_dx-2026-0094_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The Snibe-NACA 25-OH Vitamin D assay demonstrated good performance and compared favorably with both LC-MS\/MS and other competitive assays, providing evidence of its high reliability in clinical diagnostic practice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/dx-2026-0094","type":"journal-article","created":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T10:55:48Z","timestamp":1789901748000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of a new non-competitive chemiluminescent immunoassay for 25-OH vitamin D measurement"],"prefix":"10.1515","author":[{"given":"Jinqing","family":"Zhong","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine , Xiamen Humanity Hospital , Xiamen , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binning","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , Xiamen Humanity Hospital , Xiamen , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihua","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , Xiamen Humanity Hospital , Xiamen , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , Xiamen Humanity Maternity Hospital , Xiamen , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , Xiamen Humanity Maternity Hospital , Xiamen , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , Xiamen Humanity Hospital , Xiamen , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , Xiamen Humanity Hospital , Xiamen , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , Xiamen Humanity Hospital , Xiamen , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huangxing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , Xiamen Humanity Hospital , Xiamen , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine , Xiamen Humanity Hospital , Xiamen , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"2026092010554258841_j_dx-2026-0094_ref_001","doi-asserted-by":"crossref","unstructured":"Hossein-nezhad, A, Holick, MF. 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