{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T16:29:43Z","timestamp":1723220983088},"reference-count":33,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>INTRODUCTION: Mediterranean spotted fever (MSF) in Bulgaria is caused by Rickettsia conorii conorii. Aim: This study aims at investigating the absolute counts of T-lymphocyte subsets in the peripheral blood of patients with MSF in order to establish relationships with disease severity. MATERIALS AND METHODS: The absolute counts of T-lymphocyte subsets were tested in the blood of 62 patients in the acute stage of MSF. They were assigned into three age and sex matched groups, based on the severity of disease - with mild, moderate or severe forms. Controls were 32 age and sex matched healthy individuals. The diagnosis was confirmed by an immunofluorescence assay. Immunophenotyping was performed using Epics XL-MCL Coulter, USA flow-cytometer. RESULTS: The absolute counts of immune competent (CD3<jats:sup>+<\/jats:sup>) cells, as well as the counts of helper\/inducer (CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>) and suppressor\/ cytotoxic (CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup>) T-cell subsets decreased in parallel with disease severity. Na\u00efve (CD4<jats:sup>+<\/jats:sup>CD45RA<jats:sup>+<\/jats:sup>) and activated memory (CD4<jats:sup>+<\/jats:sup>CD45RO<jats:sup>+<\/jats:sup>) T-cell subsets were reduced, particularly in severe MSF. Taken as a whole, the counts of activated (CD3<jats:sup>+<\/jats:sup>HLA-DR<jats:sup>+<\/jats:sup>) and that of presenting accessory (CD28<jats:sup>+<\/jats:sup>) or stimulatory (CD38<jats:sup>+<\/jats:sup>) molecules \u0422-cell subsets was increased, but in the first two subsets the trend from mild to severe forms of the disease was descending. CONCLUSION: Reduced T-lymphocyte subset counts are likely related to trans-migration into perivascular inflammatory foci. The increased number of T-lymphocytes bearing activation molecules reflects a mobilization of the cell-mediated immune response. An important issue of this study is the possible prognostic value of T-cell subsets counting, predicting the evolution of a clinical condition to clinical forms, according to the disease severity.<\/jats:p>","DOI":"10.1515\/folmed-2015-0026","type":"journal-article","created":{"date-parts":[[2016,3,2]],"date-time":"2016-03-02T08:25:05Z","timestamp":1456907105000},"page":"93-103","source":"Crossref","is-referenced-by-count":3,"title":["Clinical Investigations. T-Lymphocyte Subset Absolute Counts in the Peripheral Blood of Mediterranean Spotted Fever Patients: Relations to Disease Severity \/ \u0410\u0431\u0441\u043e\u043b\u044e\u0442\u043d\u043e\u0435 \u041a\u043e\u043b\u0438\u0447\u0435\u0441\u0442\u0432\u043e \u0421\u0443\u0431\u043f\u043e\u043f\u0443\u043b\u044f\u0446\u0438\u0439 \u0422-\u041b\u0438\u043c\u0444\u043e\u0446\u0438\u0442\u043e\u0432 \u0412 \u041f\u0435\u0440\u0438\u0444\u0435\u0440\u0438- \u0427\u0435\u0441\u043a\u043e\u0439 \u041a\u0440\u043e\u0432\u0438 \u041f\u0430\u0446\u0438\u0435\u043d\u0442\u043e\u0432 \u0421\u043e \u0421\u0440\u0435\u0434\u0438\u0437\u0435\u043c\u043d\u043e\u043c\u043e\u0440\u0441\u043a\u043e\u0439 \u041f\u044f\u0442\u043d\u0438\u0441\u0442\u043e\u0439 \u041b\u0438\u0445\u043e- \u0420\u0430\u0434\u043a\u043e\u0439: \u0423\u0441\u0442\u0430\u043d\u043e\u0432\u043b\u0435\u043d\u0438\u0435 \u0421\u0432\u044f\u0437\u0438 \u0421 \u0422\u044f\u0436\u0435\u0441\u0442\u044c\u044e \u0417\u0430\u0431\u043e\u043b\u0435\u0432\u0430\u043d\u0438\u044f"],"prefix":"10.1515","volume":"57","author":[{"given":"Ivan G.","family":"Baltadzhiev","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavel I.","family":"Pavlov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref81","doi-asserted-by":"crossref","first-page":"53","DOI":"10.2478\/v10153-012-0006-4","article-title":"Mediterranean spotted fever - classification by disease course and criteria for determining the disease severity","volume":"54","author":"Baltadzhiev","year":"2012","journal-title":"Folia Medica"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/S1286-4579(02)01581-2","article-title":"Mechanisms of immunity against rickettsiae : new perspectives and opportunities offered by unusual intracellular parasite","volume":"4","author":"Valbuena","year":"2002","journal-title":"Microbes Infect"},{"key":"ref261","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/967852","article-title":"New insight into immunity and immunopathology of Rickettsial diseases Article ID pages","volume":"26","author":"Mansueto","year":"2012","journal-title":"Clin Dev Immunol"},{"key":"ref301","first-page":"590","article-title":"CD : an ecto - enzyme at the crossroads of innate and adaptive immune responses","volume":"38","author":"Partid\u00e1","year":"2007","journal-title":"Adv Exp Med Biol"},{"key":"ref371","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1023\/A:1014256417651","article-title":"CD function : a balance of costimulatory and regulatory signals","volume":"28","author":"Bour","year":"2002","journal-title":"J Clin Immunol"},{"key":"ref361","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1160\/TH09-03-0186","article-title":"Infection of the endothelium by members of the order rickettsiales","volume":"102","author":"Valbuena","year":"2009","journal-title":"Thromb Haemost"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"17","DOI":"10.2478\/folmed-2013-0023","article-title":"Levels of certain endothelial biomarkers during the acute phase and convalescence in patients with different severity of Mediterranean spotted fever","volume":"55","author":"Baltadzhiev","year":"2013","journal-title":"Folia Medica"},{"key":"ref191","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1111\/j.1365-4632.2004.01560.x","article-title":"Histopathologic features of and lymphoid population in the skin of patients with the spotted fever group of rickettsiae : Southern Africa","volume":"43","author":"Kim","year":"2004","journal-title":"Int J Dermatol"},{"key":"ref231","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1182\/blood-2003-08-2717","article-title":"Cognate recognition of the endothelium induces HYspecific CD lymphocyte transendothelial migration ( diapedesis ) in vivo","volume":"8","author":"Marelli","year":"2004","journal-title":"Blood"},{"key":"ref241","first-page":"197","article-title":"Presentation of antigen by endothelial cells and chemoattraction are required for homing of insulin - 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threatening complications due to Mediterranean Spotted Fever in returning travelers","volume":"17","author":"Demeester","year":"2010","journal-title":"Journal of Travel Medicine"},{"key":"ref281","first-page":"263","article-title":"Changes in the activated cytotoxic suppressor T - lymphocytes in elderly patients with Mediterranean spotted fever The","volume":"2","author":"Gospodinova","year":"2009","journal-title":"Archives International Journal of Medicine"},{"key":"ref111","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1146\/annurev.iy.11.040193.001203","article-title":"The role of the CD receptor during T cell responses to antigen","volume":"11","author":"Linsley","year":"1993","journal-title":"Annu Rev Immunol"},{"key":"ref151","first-page":"195","article-title":"T lymphocyte subsets in Mediterranean spotted fever","volume":"45","author":"Herrero","year":"1988","journal-title":"Acta Trop"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1128\/CVI.00168-07","article-title":"Evaluation of a single platform technology for lymphocyte immunophenotyping","volume":"14","author":"Higgins","year":"2007","journal-title":"Clin Vaccine Immunol"},{"key":"ref221","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/BF02786490","article-title":"Immunology of human vascular endothelium","volume":"19","author":"Pober","year":"1999","journal-title":"Immunol Res"},{"key":"ref101","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1152\/physrev.00035.2007","article-title":"Evolution and function of the ADP ribosyl cyclase \/ CD gene family in physiology and pathology","volume":"88","author":"Malavasi","year":"2008","journal-title":"Physiol Rev"},{"key":"ref251","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1086\/518145","article-title":"Rickettsiae and Rickettsial infections : The current state of knowledge","volume":"45","author":"Walker","year":"2007","journal-title":"Clin Infect Dis"},{"key":"ref351","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1615\/CritRevImmunol.v18.i5.10","article-title":"CD costimulation : a review","volume":"28","author":"Greenfield","year":"1998","journal-title":"Crit Rev Immunol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"323","DOI":"10.2217\/fmb.09.6","article-title":"Host - cell interaction with pathogenic Rickettsia sp","volume":"4","author":"Sahni","year":"2009","journal-title":"Future Microbiol"},{"key":"ref381","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/j.1440-1711.2004.01204.x","article-title":"Lack of CD expression on HIV - specific cytotoxic T - lymphocytes is associated with disease progression","volume":"82","author":"Gamberg","year":"2004","journal-title":"Immunology and Cell Biology"},{"key":"ref341","first-page":"171","article-title":"Dendritic cells stimulate primary human cytolytic lymphocyte responses in the absence of CD helper T cells","author":"Young","year":"1990","journal-title":"J Exp Med"},{"key":"ref331","first-page":"8","article-title":"CD interactions allow the induction of CD cytotoxic T lymphocytes in the absence of exogenous help","volume":"28","author":"Harding","year":"1993","journal-title":"J Exp Med"},{"key":"ref291","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1189\/jlb.0404262","article-title":"CD is expressed selectively during the activation of a subset of mature T cells with reduced proliferation but improved potential to produce cytokines","volume":"38","author":"Sandoval","year":"2005","journal-title":"J Leukoc Biol"}],"container-title":["Folia Medica"],"original-title":[],"link":[{"URL":"http:\/\/content.sciendo.com\/view\/journals\/folmed\/57\/2\/article-p93.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/view\/j\/folmed.2015.57.issue-2\/folmed-2015-0026\/folmed-2015-0026.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T09:05:54Z","timestamp":1603271154000},"score":1,"resource":{"primary":{"URL":"http:\/\/archive.sciendo.com\/FOLMED\/folmed.2015.57.issue-2\/folmed-2015-0026\/folmed-2015-0026.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,1]]},"references-count":33,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1515\/folmed-2015-0026","relation":{},"ISSN":["1314-2143"],"issn-type":[{"value":"1314-2143","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,1]]}}}