{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T07:45:06Z","timestamp":1783496706718,"version":"3.55.0"},"reference-count":28,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Background:<\/jats:bold> Alcohol-induced cerebellar neurodegeneration is a neuroadaptation that is associated with chronic alcohol abuse. Conventional drugs have been largely unsatisfactory in preventing neurodegeneration. Yet, multimodal neuro-protective therapeutic agents have been hypothesised to have high therapeutic potential for the treatment of CNS conditions; there is yet a dilemma of how this would be achieved. Contrarily, medicinal botanicals are naturally multimodal in their mechanism of action.<\/jats:p>\n<jats:p><jats:bold>Aim:<\/jats:bold> The effect of <jats:italic>L. owariensis<\/jats:italic> was therefore assessed in alcohol-induced neurodegeneration of the cerebellar cortex in rats.<\/jats:p>\n<jats:p><jats:bold>Materials and methods:<\/jats:bold> Two groups of rats were oro-gastrically fed thrice daily with 5 g\/kg ethanol (25% w\/v), and 5 g\/kg ethanol (25% w\/v) plus <jats:italic>L. owariensis<\/jats:italic> (100 mg\/kg body weight) respectively in diluted nutritionally complete diet (50% v\/v). A control group was correspondingly fed a nutritionally complete diet (50% v\/v) made isocaloric with glucose. Cytoarchitectural study of the cerebellar cortex was examined with H&amp;E. Immunocytochemical analysis was carried out with the use of monoclonal antibody anti-NF in order to detect alterations in the neuronal cytoskeleton.<\/jats:p>\n<jats:p><jats:bold>Results:<\/jats:bold> After 4 days of binge alcohol treatment, we observed that <jats:italic>L. owariensis<\/jats:italic> supplementation significantly lowered the levels of histologic and biochemical indices of neurodegeneration. The level of neurodegeneration and cytoarchitecture distortion of the cerebellar cortex of rats exposed to ethanol was reduced by <jats:italic>L. owariensis<\/jats:italic>. Neurofilament-immunoreactivity (NF-IR) was evoked in the Purkinje cells of rats that received <jats:italic>L. owariensis<\/jats:italic> supplement.<\/jats:p>\n<jats:p><jats:bold>Conclusions:<\/jats:bold> <jats:italic>L. owariensis<\/jats:italic> attenuates alcohol-induced cerebellar degeneration in the rat by alleviating oxidative stress and alteration of NF protein expression in the Purkinje cells.<\/jats:p>","DOI":"10.1515\/folmed-2016-0034","type":"journal-article","created":{"date-parts":[[2017,1,9]],"date-time":"2017-01-09T10:01:38Z","timestamp":1483956098000},"page":"241-249","source":"Crossref","is-referenced-by-count":5,"title":["Landolphia owariensis Attenuates Alcohol-induced Cerebellar Neurodegeneration: Significance of Neurofilament Protein Alteration in the Purkinje Cells"],"prefix":"10.1515","volume":"58","author":[{"given":"Charles A.","family":"Oyinbo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick S.","family":"Igbigbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Godwin O.","family":"Avwioro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"ref121","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1038\/nrn1955","article-title":"Therapeutic interventions after spinal cord injury","volume":"7","author":"Thuret","year":"2006","journal-title":"Nat Rev Neurosci"},{"key":"ref271","first-page":"58","article-title":"Neurofilamentopathy in neurodegenerative diseases The Open","volume":"5","author":"Liu","year":"2011","journal-title":"Neurology Journal"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s00401-006-0059-7","article-title":"Frequency and clinicopathological characteristics of alcoholic cerebellar degeneration in Japan : a cross - sectional study of postmortems","volume":"112","author":"Yokota","year":"2006","journal-title":"Acta Neuropathol"},{"key":"ref191","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1177\/29.4.6166661","article-title":"Use of avidin - biotin peroxidase complex ( ABC ) in immunoperoxidase techniques : A comparison between ABC and unlabeled antibody ( PAP ) procedures","volume":"29","author":"Hsu","year":"1981","journal-title":"J Histochem Cytochem"},{"key":"ref181","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/BF01234480","article-title":"A new approach to practical acute toxicity testing","volume":"54","author":"Lorke","year":"1983","journal-title":"Arch Toxicol"},{"key":"ref241","doi-asserted-by":"crossref","first-page":"65","DOI":"10.2174\/157015909787602823","article-title":"Oxidative stress and neurodegenerative diseases : a review of upstream and downstream antioxidant therapeutic options","volume":"7","author":"Uttara","year":"2009","journal-title":"Current Neuropharmacology"},{"key":"ref151","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1073\/pnas.1300083110","article-title":"Alleviation of chronic pain following rat spinal cord compression injury with multimodal actions of huperzine A","volume":"110","author":"Yu","year":"2013","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref101","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1111\/j.1369-1600.2012.00454.x","article-title":"Medications development to treat alcohol dependence : a vision for the next decade","volume":"17","author":"Litten","year":"2012","journal-title":"Addiction Biology"},{"key":"ref221","doi-asserted-by":"crossref","first-page":"258","DOI":"10.5607\/en.2013.22.4.258","article-title":"Curcumin can prevent the changes in cerebellar structure and function induced by sodium metabisulfite in rat","volume":"22","author":"Noorafshan","year":"2013","journal-title":"Experimental Neurobiology"},{"key":"ref01","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1093\/alcalc\/agn102","article-title":"The neuropathology of alcohol - related brain damage","volume":"44","author":"Harper","year":"2009","journal-title":"Alcohol"},{"key":"ref111","first-page":"80","article-title":"Dietary supplements and nutraceuticals In editors Molecular Nutrition : The Practical Guide","author":"Mechanick","year":"2015","journal-title":"The Endocrine Society"},{"key":"ref201","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/0014-5793(82)80377-3","article-title":"Copper - dependent hydroxyl radical damage to ascorbic acid : Formation of a thiobarbituric acid - reactive product","volume":"137","author":"Gutteridge","year":"1982","journal-title":"FEBS Lett"},{"key":"ref211","first-page":"461","article-title":"Memory function and total pyramidal neuron number of hippocampus in streptozotocin induced diabetic rats","volume":"31","author":"Yonguc","year":"2014","journal-title":"J Neurol Sci Turk"},{"key":"ref281","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1242\/jcs.104729","article-title":"Neurofilaments at a glance","volume":"125","author":"Yuan","year":"2012","journal-title":"Journal of Cell Science"},{"key":"ref231","first-page":"389","article-title":"Neuroprotective effect of total and sequential extract ofScrophularia striata Boiss in rat cerebellar granule neurons following glutamate - induced neurotoxicity : an in - vitro study","volume":"12","author":"Salavati","year":"2013","journal-title":"Iran J Pharm Res"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0091-3057(02)00715-3","article-title":"Cognitive deficits and CNS damage after a - day binge ethanol exposure in rats","volume":"72","author":"Obernier","year":"2002","journal-title":"Pharmacol Biochem Behav"},{"key":"ref291","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1111\/bpa.12230","article-title":"Purkinje cell pathology and loss in multiple sclerosis cerebellum","volume":"25","author":"Redondo","year":"2015","journal-title":"Brain Pathology"},{"key":"ref251","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/1471-2202-11-98","article-title":"Hydroxysafflor Yellow A protects spinal cords from ischemia \/ reperfusion injury in rabbits","volume":"11","author":"Shan","year":"2010","journal-title":"BMC neuroscience"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.cortex.2012.05.021","article-title":"Pathways to alcohol - induced brain impairment in young people : a review","volume":"49","author":"Hermens","year":"2013","journal-title":"Cortex"},{"key":"ref131","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1038\/sj.sc.3100962","article-title":"Pharmacological therapy of spinal cord injury during the acute phase","volume":"38","author":"Pointillart","year":"2000","journal-title":"Spinal Cord"},{"key":"ref51","first-page":"1007","article-title":"Reduction of Purkinje cell volume in cerebellum of alcoholics","author":"Andersen","year":"2004","journal-title":"Brain research"},{"key":"ref91","first-page":"1994","article-title":"Traumatic brain injury and substance misuse : a systematic review of prevalence and outcomes research","volume":"16","author":"Parry","year":"2004","journal-title":"Neuropsychol Rehabil"},{"key":"ref161","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.2174\/0929867033456729","article-title":"Virtual screening on natural products for discovering active compounds and target information","volume":"10","author":"Shen","year":"2003","journal-title":"Current Medicinal Chemistry"},{"key":"ref171","first-page":"28","article-title":"Isolation and characterization of potential bioactive compounds fromL owariensisP Beauv Stringy Seed Pulp of Applied Research in Natural Products","volume":"6","author":"Okonkwo","year":"2013","journal-title":"International Journal"},{"key":"ref261","doi-asserted-by":"crossref","first-page":"245","DOI":"10.2298\/ABS1401245J","article-title":"Antioxidative defense mechanisms in the aging brain","volume":"66","author":"Jovanovic","year":"2014","journal-title":"Archives of Biological Sciences"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1007\/s12311-008-0034-z","article-title":"Mechanisms of ethanol - induced degeneration in the developing , mature , and aging cerebellum","volume":"7","author":"Jaatinen","year":"2008","journal-title":"Cerebellum"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1111\/j.1530-0277.2009.00953.x","article-title":"Altered white matter integrity in adolescent binge drinkers","volume":"33","author":"McQueeny","year":"2009","journal-title":"Alcohol Clin Exp Res"},{"key":"ref141","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.expneurol.2014.02.021","article-title":"The past , present and future of stem cell clinical trials for ALS","volume":"262","author":"Thomsen","year":"2014","journal-title":"Experimental neurology"}],"container-title":["Folia Medica"],"original-title":[],"link":[{"URL":"http:\/\/content.sciendo.com\/view\/journals\/folmed\/58\/4\/article-p241.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.degruyter.com\/view\/j\/folmed.2016.58.issue-4\/folmed-2016-0034\/folmed-2016-0034.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T09:06:19Z","timestamp":1603271179000},"score":1,"resource":{"primary":{"URL":"http:\/\/archive.sciendo.com\/FOLMED\/folmed.2016.58.issue-4\/folmed-2016-0034\/folmed-2016-0034.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,1]]},"references-count":28,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1515\/folmed-2016-0034","relation":{},"ISSN":["1314-2143"],"issn-type":[{"value":"1314-2143","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,1]]}}}