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Therefore, this enzyme is a potential target for developing new anti-infectives. As species specificity of such compounds is required in order to avoid off-target or adverse effects, comparative analysis of the gGTs from different organisms is a prerequisite for drug development. To allow detailed biochemical and enzymatic characterization, recombinant gGTs from five different bacteria as well as <jats:italic>Homo sapiens<\/jats:italic> were characterized and compared. Investigation of the enzymatic activity, the binding modes of known inhibitors to the catalytic center, and a high resolution X-ray structure of the HpgGT provided a starting point for the identification of new inhibitory substances targeting HpgGT. Inhibitors with <jats:italic>K<\/jats:italic>\n                  <jats:sub>i<\/jats:sub> values in the n<jats:sc>m<\/jats:sc> to m<jats:sc>m<\/jats:sc> range were identified and their binding modes were analyzed by mass spectrometry. The results of this study provide a basis for the development of species-specific lead compounds with anti-infective potential by effectively inhibiting HpgGT.<\/jats:p>","DOI":"10.1515\/hsz-2016-0198","type":"journal-article","created":{"date-parts":[[2016,9,16]],"date-time":"2016-09-16T14:15:36Z","timestamp":1474035336000},"page":"341-357","source":"Crossref","is-referenced-by-count":6,"title":["Comparison of enzymatic properties and small molecule inhibition of \u03b3\u2013glutamyltranspeptidases from pathogenic and commensal bacteria"],"prefix":"10.1515","volume":"398","author":[{"given":"Christian","family":"Bolz","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universit\u00e4t M\u00fcnchen, Trogerstrasse 30, D-81675 M\u00fcnchen, Germany"},{"name":"ImevaX GmbH, Grillparzerstra\u00dfe 18, D-81675 M\u00fcnchen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina C.","family":"Bach","sequence":"additional","affiliation":[{"name":"Center for Integrated Protein Science (CIPSM), Department of Chemistry, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstra\u00dfe 4, D-85748 Garching, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannelore","family":"Meyer","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universit\u00e4t M\u00fcnchen, Trogerstrasse 30, D-81675 M\u00fcnchen, Germany"},{"name":"German Center for Infection Research (DZIF), Trogerstrasse 30, D-81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Mercachem, NL-6546 Nijmegen, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maciej","family":"Dawidowski","sequence":"additional","affiliation":[{"name":"Institute of Structural Biology, Helmholtz Zentrum M\u00fcnchen, Ingolst\u00e4dter Landstra\u00dfe 1, D-85764 Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"Popowicz","sequence":"additional","affiliation":[{"name":"Institute of Structural Biology, Helmholtz Zentrum M\u00fcnchen, Ingolst\u00e4dter Landstra\u00dfe 1, D-85764 Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan A.","family":"Sieber","sequence":"additional","affiliation":[{"name":"Center for Integrated Protein Science (CIPSM), Department of Chemistry, Technische Universit\u00e4t M\u00fcnchen, Lichtenbergstra\u00dfe 4, D-85748 Garching, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arne","family":"Skerra","sequence":"additional","affiliation":[{"name":"Munich Center for Integrated Protein Science (CIPS-M) and Lehrstuhl f\u00fcr Biologische Chemie, Technische Universit\u00e4t M\u00fcnchen, D-85354 Freising (Weihenstephan), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Gerhard","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universit\u00e4t M\u00fcnchen, Trogerstrasse 30, D-81675 M\u00fcnchen, Germany"},{"name":"ImevaX GmbH, Grillparzerstra\u00dfe 18, D-81675 M\u00fcnchen, Germany"},{"name":"German Center for Infection Research (DZIF), Trogerstrasse 30, D-81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2016,9,15]]},"reference":[{"key":"2023033114015701382_j_hsz-2016-0198_ref_001_w2aab3b7c44b1b6b1ab2b1b1Aa","doi-asserted-by":"crossref","unstructured":"Ahluwalia, G.S., Grem, J.L., Hao, Z., and Cooney, D.A. 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