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We investigated the pH dependence of electron transfer and generation of a transmembrane voltage (\u0394\u03a8) by NQR reconstituted in liposomes with Na<jats:sup>+<\/jats:sup> or Li<jats:sup>+<\/jats:sup> as coupling cation. \u0394\u03a8 formation was followed with the voltage-sensitive dye oxonol. With Na<jats:sup>+<\/jats:sup>, \u0394\u03a8 was barely influenced by pH (6.5\u20138.5), while Q reduction activity exhibited a maximum at pH 7.5\u20138.0. With Li<jats:sup>+<\/jats:sup>, \u0394\u03a8 was generally lower, and the pH profile of electron transfer activity did not reveal a pronounced maximum. We conclude that the coupling efficiency of NQR is influenced by the nature of the transported cation, and by the concentration of protons. The 3D structure of NQR reveals a transmembrane channel in subunit NqrB. It is proposed that partial uncoupling of the NQR observed with the smaller Li<jats:sup>+<\/jats:sup>, or with Na<jats:sup>+<\/jats:sup> at pH 7.5\u20138.0, is caused by the backflow of the coupling cation through the channel in NqrB.<\/jats:p>","DOI":"10.1515\/hsz-2016-0238","type":"journal-article","created":{"date-parts":[[2016,9,19]],"date-time":"2016-09-19T11:30:11Z","timestamp":1474284611000},"page":"251-260","source":"Crossref","is-referenced-by-count":11,"title":["Strong pH dependence of coupling efficiency of the Na<sup>+<\/sup> \u2013 translocating NADH:quinone oxidoreductase (Na<sup>+<\/sup>-NQR) of <i>Vibrio cholerae<\/i>"],"prefix":"10.1515","volume":"398","author":[{"given":"Charlotte","family":"Toulouse","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Hohenheim (Stuttgart), Garbenstrasse 30, D-70599 Stuttgart, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bj\u00f6rn","family":"Claussen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Hohenheim (Stuttgart), Garbenstrasse 30, D-70599 Stuttgart, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentin","family":"Muras","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Hohenheim (Stuttgart), Garbenstrasse 30, D-70599 Stuttgart, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00fcnter","family":"Fritz","sequence":"additional","affiliation":[{"name":"Institute for Neuropathology, University of Freiburg, Breisacherstr. 64, D-79106 Freiburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Steuber","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Hohenheim (Stuttgart), Garbenstrasse 30, D-70599 Stuttgart, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2016,9,16]]},"reference":[{"key":"2023033113252708255_j_hsz-2016-0238_ref_001_w2aab3b7c29b1b6b1ab2b1b1Aa","doi-asserted-by":"crossref","unstructured":"Apell, H.J. and Bersch, B. 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