{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T12:05:57Z","timestamp":1782821157583,"version":"3.54.5"},"reference-count":23,"publisher":"Walter de Gruyter GmbH","issue":"5","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31602600"],"award-info":[{"award-number":["31602600"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,5,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study aims to investigate the mechanism of miR-23a-3p in regulating Treg dysfunction in Graves\u2019 disease (GD). The percentage of Treg cells and interleukin (IL)-17<jats:sup>+<\/jats:sup> T cells were determined by flow cytometry. The expression of forkhead box P3 (<jats:italic>FOXP3<\/jats:italic>), sirtuin 1 (<jats:italic>SIRT1<\/jats:italic>), RAR-related orphan receptor gamma t (<jats:italic>ROR\u03b3t<\/jats:italic>) and miR-23a-3p was analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) or Western blot. CD4<jats:sup>+<\/jats:sup> T cells were treated with SIRT1 specific inhibitor EX-527 or left untreated. MiR-23a-3p mimic or inhibitor were transfected into CD4<jats:sup>+<\/jats:sup> T cells. Acetylation expression of FOXP3 was analyzed by immunoprecipitation. The suppressive function of Treg was analyzed by the carboxyfluorescein succinimidyl ester (CFSE) assay. The results showed that GD patients have significantly less Treg cells and more IL-17<jats:sup>+<\/jats:sup> T cells. FOXP3 and miR-23a-3p were significantly down-regulated meanwhile SIRT1 and ROR\u03b3t were up-regulated in GD patients. FOXP3 acetylation level of the GD group was lower than that of control groups. After EX-527 treatment, the percentage of Treg cells, expression and acetylation level of FOXP3 were significantly increased in the GD group. GD Tregs exhibited weaker suppressive activity, miR-23a-3p mimic suppressed SIRT1 expression and suppressive-activity of Tregs whereas it promoted the expression and acetylation level of FOXP3 in the GD group. Our findings suggest that the Treg function defect in GD patients is mediated by the abnormal acetylation of FOXP3, which is regulated by miR-23a-3p via targeting SIRT1.<\/jats:p>","DOI":"10.1515\/hsz-2018-0343","type":"journal-article","created":{"date-parts":[[2018,11,3]],"date-time":"2018-11-03T18:03:23Z","timestamp":1541268203000},"page":"639-650","source":"Crossref","is-referenced-by-count":27,"title":["MiR-23a-3p-regulated abnormal acetylation of FOXP3 induces regulatory T cell function defect in Graves\u2019 disease"],"prefix":"10.1515","volume":"400","author":[{"given":"Danhua","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of General surgery , The First Affiliated Hospital of Zhengzhou University , No. 41 Jianshe Road, Erqi District , Zhengzhou 450052, Henan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinguang","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of General surgery , The First Affiliated Hospital of Zhengzhou University , No. 41 Jianshe Road, Erqi District , Zhengzhou 450052, Henan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General surgery , The First Affiliated Hospital of Zhengzhou University , No. 41 Jianshe Road, Erqi District , Zhengzhou 450052, Henan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shouhua","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of General surgery , The First Affiliated Hospital of Zhengzhou University , No. 41 Jianshe Road, Erqi District , Zhengzhou 450052, Henan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General surgery , The First Affiliated Hospital of Zhengzhou University , No. 41 Jianshe Road, Erqi District , Zhengzhou 450052, Henan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongchao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of General surgery , The First Affiliated Hospital of Zhengzhou University , No. 41 Jianshe Road, Erqi District , Zhengzhou 450052, Henan Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2018,12,3]]},"reference":[{"key":"2023040406520414415_j_hsz-2018-0343_ref_001_w2aab3b7c56b1b6b1ab2ab1Aa","doi-asserted-by":"crossref","unstructured":"Akimova, T., Xiao, H., Liu, Y., Bhatti, T.R., Jiao, J., Eruslanov, E., Singhal, S., Wang, L., Han, R., Zacharia, K., et al. 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