{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T12:28:29Z","timestamp":1770726509039,"version":"3.49.0"},"reference-count":44,"publisher":"Walter de Gruyter GmbH","issue":"9","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31401807"],"award-info":[{"award-number":["31401807"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In the process of immobilizing the enzyme, the overflow of enzyme and the decrease of enzyme activity are very serious. In order to improve the stable binding between enzyme and carrier, a kind of porous magnetic chitosan microsphere with appropriate pore size was successfully prepared by adjusting the size of pore-forming agent in this paper. The rough porous structure is favorable for the adsorption of enzyme and the catalytic action of enzyme on substrate. The results showed that when the pore size of the microspheres was at 790.15\u00a0\u00b1\u00a0250.91\u00a0nm, the protein loading and enzyme activity of GOD could be increased effectively, which could reach 58.28\u00a0\u00b1\u00a02.64\u00a0mg\/g and 16.93\u00a0\u00b1\u00a00.14 U, respectively. The co-immobilization of CAT and GOD eliminated the harmful by-product H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    in time and effectively solved the problem of continuous deactivation of GOD in the reaction process. When the mass ratio of PMCSM\/GOD\/CAT was 100\/6.02\/10.96 (mg\/mg\/mg), the relative enzyme activity of GOD reached the highest (133.32\u00a0\u00b1\u00a00.68%). The thermal stability and pH stability of the enzyme were greatly improved after co-immobilization. The relative enzyme activity of PMCSM@GOD@CAT was 57.27\u00a0\u00b1\u00a03.04% at 60\u00a0\u00b0C, while that of free GOD was only 28.76\u00a0\u00b1\u00a04.10%. The relative enzyme activity of PMCSM@GOD@CAT was above 63% at pH 5\u201310, while the relative enzyme activity of free GOD was only 4.98\u00a0\u00b1\u00a00.72% at pH 10. The yield of sodium gluconate from 50\u00a0mL 250\u00a0mg\/mL glucose catalyzed by PMCSM@GOD@CAT loading 60.2\u00a0mg GOD was 96.19\u00a0\u00b1\u00a00.79% at pH 6.0 and 30\u00a0\u00b0C, and the reaction lasted 6\u00a0h. The relative enzyme activity of PMCSM@GOD@CAT remained 69.77\u00a0\u00b1\u00a00.78% after repeated use for 10 times. After 30\u00a0days of storage, PMCSM@GOD@CAT maintained its initial activity of 76.52\u00a0\u00b1\u00a01.41%. The immobilized process studied in this paper provides a theoretical basis for the production of sodium gluconate by double enzyme directly catalyzing and lays a certain foundation for the application of immobilized enzyme in the future chemical industry and food industry.\n                  <\/jats:p>","DOI":"10.1515\/ijcre-2021-0237","type":"journal-article","created":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T17:57:15Z","timestamp":1646416635000},"page":"989-1001","source":"Crossref","is-referenced-by-count":13,"title":["Co-immobilization of glucose oxidase and catalase in porous magnetic chitosan microspheres for production of sodium gluconate"],"prefix":"10.1515","volume":"20","author":[{"given":"Youcai","family":"Liu","sequence":"first","affiliation":[{"name":"School of Bioengineering and Food , Hubei University of Technology , Wuhan , 430068 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengpeng","family":"Zou","sequence":"additional","affiliation":[{"name":"School of Bioengineering and Food , Hubei University of Technology , Wuhan , 430068 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Bioengineering and Food , Hubei University of Technology , Wuhan , 430068 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Bioengineering and Food , Hubei University of Technology , Wuhan , 430068 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2022,3,7]]},"reference":[{"key":"2025121207412232718_j_ijcre-2021-0237_ref_001","doi-asserted-by":"crossref","unstructured":"Akhmetov, M. M., G. G. Gumarov, V. Y. Petukhov, and M. Y. Volkov. 2019. \u201cNMR Study of Sodium Gluconate Solutions.\u201d Journal of Molecular Structure 1193: 373\u20137, https:\/\/doi.org\/10.1016\/j.molstruc.2019.05.061.","DOI":"10.1016\/j.molstruc.2019.05.061"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_002","doi-asserted-by":"crossref","unstructured":"Arana-Pea, S., D. Carballares, R. Morellon-Sterlling, N. Berenguer-Murcia, and R. Fernandez-Lafuente. 2020. \u201cEnzyme Co-immobilization: Always the Biocatalyst Designers\u2019 Choice or Not?.\u201d Biotechnology Advances 51: 107584, https:\/\/doi.org\/10.1016\/j.biotechadv.2020.107584.","DOI":"10.1016\/j.biotechadv.2020.107584"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_003","doi-asserted-by":"crossref","unstructured":"Bankar, S. B., M. V. Bule, R. S. Singhal, and L. Ananthanarayan. 2009. \u201cGlucose Oxidase\u2013an Overview.\u201d Biotechnology Advances 27 (4): 489\u2013507, https:\/\/doi.org\/10.1016\/j.biotechadv.2009.04.003.","DOI":"10.1016\/j.biotechadv.2009.04.003"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_004","doi-asserted-by":"crossref","unstructured":"Burstein, G. T. 1997. \u201cThe Iron Oxides: Structure, Properties, Reactions, Occurrence and Uses.\u201d Corrosion Science 39 (8): 1499\u2013500, https:\/\/doi.org\/10.1016\/S0010-938X(97)00096-6.","DOI":"10.1016\/S0010-938X(97)00096-6"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_005","doi-asserted-by":"crossref","unstructured":"Cao, S. L., X. H. Li, and W. Y. Lou. 2014. \u201cPreparation of a Novel Magnetic Cellulose Nanocrystal and its Efficient Use for Enzyme Immobilization.\u201d Journal of Materials Chemistry B 2: 5522\u201330, https:\/\/doi.org\/10.1039\/c4tb00584h.","DOI":"10.1039\/C4TB00584H"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_006","doi-asserted-by":"crossref","unstructured":"Chung, Y., M. Christwardana, D. C. Tannia, K. J. Kim, and Y. Kwon. 2017. \u201cBiocatalyst Including Porous Enzyme Cluster Composite Immobilized by Two-step Crosslinking and its Utilization as Enzymatic Biofuel Cell.\u201d Journal of Power Sources 360: 172\u20139, https:\/\/doi.org\/10.1016\/j.jpowsour.2017.06.012.","DOI":"10.1016\/j.jpowsour.2017.06.012"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_007","doi-asserted-by":"crossref","unstructured":"Cui, C., Y. Fang, B. Chen, and T. Tan. 2019. \u201cGlucose Oxidation Performance Is Improved by the Use of a Supramolecular Self-assembly of Glucose Oxidase and Catalase.\u201d Catalysis Science and Technology 9 (2): 477\u201382, https:\/\/doi.org\/10.1039\/C8CY01945B.","DOI":"10.1039\/C8CY01945B"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_008","doi-asserted-by":"crossref","unstructured":"Darwish, S. S., M. E. Hassan, H. E. Ahmed, M. A. E. Fadl, and M. B. EI Bhery. 2021. \u201cEvaluation of Effectiveness of Covalently Immobilized \u03b1-Amylase and Lipase in Cleaning of Historical Textiles.\u201d Biointerface Research in Applied Chemistry 11: 9952\u201362, https:\/\/doi.org\/10.33263\/BRIAC113.99529962.","DOI":"10.33263\/BRIAC113.99529962"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_009","doi-asserted-by":"crossref","unstructured":"Deng, W., S. Tang, X. Zhou, Y. Liu, and J. Luo. 2020. \u201cHoneycomb-like Structure-tunable Chitosan-based Porous Carbon Microspheres for Methylene Blue Efficient Removal.\u201d Carbohydrate Polymers 247 (9): 116736, https:\/\/doi.org\/10.1016\/j.carbpol.2020.116736.","DOI":"10.1016\/j.carbpol.2020.116736"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_010","doi-asserted-by":"crossref","unstructured":"Dml, A., and D. B. Chen. 2020. \u201cRecent Advances in Nano-carrier Immobilized Enzymes and their Applications.\u201d Process Biochemistry 92: 464\u201375, https:\/\/doi.org\/10.1016\/j.procbio.2020.02.005.","DOI":"10.1016\/j.procbio.2020.02.005"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_011","doi-asserted-by":"crossref","unstructured":"Engkagul, V., I. Y. Klaharn, A. Sereemaspun, and S. Chirachanchai. 2017. \u201cChitosan Whisker Grafted with Oligo(lactic Acid) Nanoparticles via a Green Synthesis Pathway: Potential as a Transdermal Drug Delivery System.\u201d Nanomedicine: Nanotechnology, Biology and Medicine 13: 2523\u201331, https:\/\/doi.org\/10.1016\/j.nano.2017.07.001.","DOI":"10.1016\/j.nano.2017.07.001"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_012","doi-asserted-by":"crossref","unstructured":"Fernandez-Lafuente, R., A. Berenguer-Murcia, J. Santos, C. Garcia-Galan, N. Rueda, O. Barbosa, K. Hernandez, and R. C. Rodrigues. 2015. \u201cImmobilization of Proteins in Poly-styrene-divinylbenzene Matrices: Functional Properties and Applications.\u201d Current Organic Chemistry 19: 1707\u201328, https:\/\/doi.org\/10.2174\/1385272819666150429231728.","DOI":"10.2174\/1385272819666150429231728"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_013","doi-asserted-by":"crossref","unstructured":"Gao, Q., Z. Li, C. Lei, R. Fu, and Z. Liu. 2020. \u201cApplication of Pulsed Electric Field in Antifouling Treatment of Sodium Gluconate Mother Liquor by Electrodialysis.\u201d Materials 13 (11): 2501, https:\/\/doi.org\/10.3390\/ma13112501.","DOI":"10.3390\/ma13112501"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_014","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Garc\u00eda, P., J. Rocha-Martin, J.\u00a0M. Guisan, and G. Fernandez-Lorente. 2020. \u201cCo-immobilization and Co-localization of Oxidases and Catalases: Catalase from Bordetella Pertussis Fused with the Zbasic Domain.\u201d Catalysts 10 (7): 810, https:\/\/doi.org\/10.3390\/catal10070810.","DOI":"10.3390\/catal10070810"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_015","doi-asserted-by":"crossref","unstructured":"Girelli, A. M., and E. Mattei. 2005. \u201cApplication of Immobilized Enzyme Reactor in On-line High Performance Liquid Chromatography: A\u00a0Review.\u201d Journal of Chromatography B 819 (1): 3\u201316, https:\/\/doi.org\/10.1016\/j.jchromb.2005.01.031.","DOI":"10.1016\/j.jchromb.2005.01.031"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_016","doi-asserted-by":"crossref","unstructured":"Guo, Z., and X. Yan. 2018. \u201cFitness Partition-based Multi-objective Differential Evolutionary Algorithm and its Application to the Sodium Gluconate Fermentation Process.\u201d Chemometrics and Intelligent Laboratory Systems 177: 8\u201316, https:\/\/doi.org\/10.1016\/j.chemolab.2018.04.006.","DOI":"10.1016\/j.chemolab.2018.04.006"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_017","doi-asserted-by":"crossref","unstructured":"Han, X., G. Liu, Y. Pan, W. Song, and Y. Qu. 2018. \u201cConsolidated Bioprocessing for Sodium Gluconate Production from Cellulose Using Penicillium oxalicum.\u201d Bioresource Technology 251: 407\u201310, https:\/\/doi.org\/10.1016\/j.biortech.2017.12.028.","DOI":"10.1016\/j.biortech.2017.12.028"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_018","doi-asserted-by":"crossref","unstructured":"Hu, M., K. Korschelt, P. Daniel, K. Landfester, W. Tremel, and M. B. Bannwarth. 2017. \u201cFibrous Nanozyme Dressings with Catalase-like Activity for H2O2 Reduction to Promote Wound Healing.\u201d ACS Appl Mater Interfaces 9 (43): 38024\u201331, https:\/\/doi.org\/10.1021\/acsami.7b12212.","DOI":"10.1021\/acsami.7b12212"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_019","doi-asserted-by":"crossref","unstructured":"Hu, T., H. Wang, L. Zhang, and S. Tao. 2019. \u201cPreparation of Hollow Silver-polymer Microspheres with a Hierarchical Structure for SERS.\u201d Applied Surface Science 490: 293\u2013301, https:\/\/doi.org\/10.1016\/j.apsusc.2019.06.061.","DOI":"10.1016\/j.apsusc.2019.06.061"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_020","doi-asserted-by":"crossref","unstructured":"Huang, Z. X., S. L. Cao, P. Xu, H. Wu, M. H. Zong, and W. Y. Lou. 2018. \u201cPreparation of a Novel Nanobiocatalyst by Immobilizing Penicillin Acylase onto Magnetic Nanocrystalline Cellulose and its Use for Efficient Synthesis of Cefaclor.\u201d Chemical Engineering Journal 346: 361\u20138, https:\/\/doi.org\/10.1016\/j.cej.2018.04.026.","DOI":"10.1016\/j.cej.2018.04.026"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_021","doi-asserted-by":"crossref","unstructured":"Ishikawa, S., D. Matsukuma, K. Iijima, M. Iijima, S. Osawa, and H. Otsuka. 2019. \u201cN-hydroxysuccinimide Bi-functionalized Triblock Cross-linker Having Hydrolysis Property for Biodegradable and Injectable Hydrogel System.\u201d ACS Biomaterials Science & Engineering 5: 5759\u201369, https:\/\/doi.org\/10.1021\/acsbiomaterials.9b00218.","DOI":"10.1021\/acsbiomaterials.9b00218"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_022","doi-asserted-by":"crossref","unstructured":"Jie, Z., X. Zhou, W. Dan, Y. Wang, and S. Tan. 2013. \u201cStudies on the Co-immobilized GOD\/CAT on Cross-linked Chitosan Microsphere Modified by Lysine.\u201d Journal of Molecular Catalysis B: Enzymatic 97: 80\u20136, https:\/\/doi.org\/10.1016\/j.molcatb.2013.07.009.","DOI":"10.1016\/j.molcatb.2013.07.009"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_023","doi-asserted-by":"crossref","unstructured":"Khandebharad, A., S. Sarda, M. Soni, and B. Agrawal. 2019. \u201cSodium Gluconate: An Efficient Organocatalyst for the Synthesis of Dihydropyrano[2,3-C] Pyrazole Derivatives.\u201d Bulletin of the Chemical Society of Ethiopia 33 (2): 331\u201340, https:\/\/doi.org\/10.4314\/bcse.v33i2.13.","DOI":"10.4314\/bcse.v33i2.13"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_024","doi-asserted-by":"crossref","unstructured":"Kornecki, J.\u00a0F., D. Carballares, P. W. Tardioli, R. C. Rodrigues, \u00c1. Berenguer-Murcia, A. R. Alc\u00e1ntara, and R. Fernandez-Lafuente. 2020. \u201cEnzyme Production of d-gluconic Acid and Glucose Oxidase: Successful Tales of Cascade Reactions.\u201d Catalysis Science and Technology 10: 5740\u201371, https:\/\/doi.org\/10.1039\/D0CY00819B.","DOI":"10.1039\/D0CY00819B"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_025","doi-asserted-by":"crossref","unstructured":"Kwon, O. H., Y.-G. Ko, W. H. Park, and C. Donghwan. 2017. \u201cPreventing Postoperative Tissue Adhesion Using Injectable.\u201d Carboxymethyl Cellulose-Pullulan Hydrogels 105: 886\u201393, https:\/\/doi.org\/10.1016\/j.ijbiomac.2017.07.103.","DOI":"10.1016\/j.ijbiomac.2017.07.103"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_026","doi-asserted-by":"crossref","unstructured":"Li, C., X. Liu, Y. Fan, H. Gang, and W. Ying. 2019. \u201cGlucose and H2O2 Dual-sensitive Nanogels for Enhanced Glucose-responsive Insulin Delivery.\u201d Nanoscale 11 (18): 9163\u201375 https:\/\/doi.org\/10.1039\/C9NR01554J.","DOI":"10.1039\/C9NR01554J"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_027","doi-asserted-by":"crossref","unstructured":"Liao, L., Y. Meng, R. Wang, B. Jia, and P. Li. 2019. \u201cCoupling and Regulation of Porous Carriers Using Plasma and Amination to Improve the Catalytic Performance of Glucose Oxidase and Catalase.\u201d Frontiers in Bioengineering and Biotechnology 7: 426\u201335, https:\/\/doi.org\/10.3389\/fbioe.2019.00426.","DOI":"10.3389\/fbioe.2019.00426"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_028","doi-asserted-by":"crossref","unstructured":"Liu, Y., Z. Cai, Y. Jin, L. Sheng, and M. Ma. 2020. \u201cVolcanic Rock-inspired Fabrication of Porous Chitosan Macroparticles via Gas Porogen for Enhancing the Activity of Immobilized Enzymes.\u201d ACS Sustainable Chemistry & Engineering 8 (41): 15560\u201372, https:\/\/doi.org\/10.1021\/acssuschemeng.0c04667.","DOI":"10.1021\/acssuschemeng.0c04667"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_029","doi-asserted-by":"crossref","unstructured":"Mafra, O., G. Ulrich, F.-L. Kornecki, and Ribeiro. 2019. \u201cCombi-CLEAs of Glucose Oxidase and Catalase for Conversion of Glucose to Gluconic Acid Eliminating the Hydrogen Peroxide to Maintain Enzyme Activity in a Bubble Column Reactor.\u201d Catalysts 9 (8): 657, https:\/\/doi.org\/10.3390\/catal9080657.","DOI":"10.3390\/catal9080657"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_030","doi-asserted-by":"crossref","unstructured":"Mej\u00eda-Ot\u00e1lvaro, F., A. Merino-Restrepo, and A. Hormaza-Anaguano. 2021. \u201cEvaluation of a Trametes Pubescens Laccase Concentrated Extract on Allura Red AC Decolorization without the Addition of Synthetic Mediators.\u201d Journal of Environmental Management 285: 112117, https:\/\/doi.org\/10.1016\/j.jenvman.2021.112117.","DOI":"10.1016\/j.jenvman.2021.112117"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_031","doi-asserted-by":"crossref","unstructured":"Miao, G., Z. Li, Y. Meng, J. Wu, Y. Li, Q. Hu, X. Chen, X. Yang, and X. Chen. 2019. \u201cPreparation, Characterization, in vitro Bioactivity and Protein Loading\/release Property of Mesoporous Bioactive Glass Microspheres with Different Compositions.\u201d Advanced Powder Technology 30 (9): 1848\u201357, https:\/\/doi.org\/10.1016\/j.apt.2019.06.002.","DOI":"10.1016\/j.apt.2019.06.002"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_032","doi-asserted-by":"crossref","unstructured":"Mota, B., T. Matschei, and K. Scrivener. 2019. \u201cImpact of Sodium Gluconate on White Cement-slag Systems with Na2SO4.\u201d Cement and Concrete Research 122: 59\u201371, https:\/\/doi.org\/10.1016\/j.cemconres.2019.04.008.","DOI":"10.1016\/j.cemconres.2019.04.008"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_033","doi-asserted-by":"crossref","unstructured":"Nakamura, I., A. Makino, M. Ohmae, and S. Kimura. 2011. \u201cEnzymatic Polymerization to Cellulose by Crosslinked Enzyme Immobilized on Gold Solid Surface.\u201d Chemistry Letters 41 (1): 37\u20138, https:\/\/doi.org\/10.1246\/cl.2012.37.","DOI":"10.1246\/cl.2012.37"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_034","doi-asserted-by":"crossref","unstructured":"S\u00e1ringer, S., P. Rouster, and I. Szilagyi. 2021. \u201cCo-immobilization of Antioxidant Enzymes on Titania Nanosheets for Reduction of Oxidative Stress in Colloid Systems.\u201d Journal of Colloid and Interface Science 590: 28\u201337, https:\/\/doi.org\/10.1016\/j.jcis.2021.01.012.","DOI":"10.1016\/j.jcis.2021.01.012"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_035","doi-asserted-by":"crossref","unstructured":"Skopinska-Wisniewska, J., M. Tuszynska, and E. Olewnik-Kruszkowska. 2021. \u201cComparative Study of Gelatin Hydrogels Modified by Various Cross-linking Agents.\u201d Materials 14 (2): 396\u2013411, https:\/\/doi.org\/10.3390\/ma14020396.","DOI":"10.3390\/ma14020396"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_036","doi-asserted-by":"crossref","unstructured":"Soares, J.\u00a0C., P. R. Moreira, A. C. Queiroga, J. Morgado, F. X. Malcata, and M. E. Pintado. 2011. \u201cApplication of Immobilized Enzyme Technologies for the Textile Industry: a Review.\u201d Biocatalysis and Biotransformation 29 (6): 223\u201337, https:\/\/doi.org\/10.3109\/10242422.2011.635301.","DOI":"10.3109\/10242422.2011.635301"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_037","doi-asserted-by":"crossref","unstructured":"Wang, A., Q. Zhu, and Z. Xing. 2020a. \u201cMultifunctional Quaternized Chitosan@surface Plasmon Resonance Ag\/N-TiO2 Core-shell Microsphere for Synergistic Adsorption-photothermal Catalysis Degradation of Low-temperature Wastewater and Bacteriostasis under Visible light\u00a0\u2013 ScienceDirect.\u201d Chemical Engineering Journal 393: 124781, https:\/\/doi.org\/10.1016\/j.cej.2020.124781.","DOI":"10.1016\/j.cej.2020.124781"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_038","doi-asserted-by":"crossref","unstructured":"Wang, S., H. Zheng, L. Zhou, F. Cheng, and Q. Zhang. 2020b. \u201cNanoenzyme-reinforced Injectable Hydrogel for Healing Diabetic Wounds Infected with Multi-drug Resistant Bacteria.\u201d Nano Letters 20: 5149\u201358, https:\/\/doi.org\/10.1021\/acs.nanolett.0c01371.","DOI":"10.1021\/acs.nanolett.0c01371"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_039","doi-asserted-by":"crossref","unstructured":"Wei, Y., J.\u00a0I. Peters, and R. Iii. 2008. \u201cInhaled Nanoparticles\u2014A Current Review.\u201d International Journal of Pharmaceutics 356 (1\u20132): 239\u201347, https:\/\/doi.org\/10.1016\/j.ijpharm.2008.02.011.","DOI":"10.1016\/j.ijpharm.2008.02.011"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_040","doi-asserted-by":"crossref","unstructured":"Wu, Y., J. Wang, S. Song, P. Rao, and F. Zhang. 2020. \u201cPreparation and Application Properties of Sustainable Gelatin\/chitosan Soil Conditioner Microspheres.\u201d International Journal of Biological Macromolecules 159: 685\u201395, https:\/\/doi.org\/10.1016\/j.ijbiomac.2020.05.122.","DOI":"10.1016\/j.ijbiomac.2020.05.122"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_041","doi-asserted-by":"crossref","unstructured":"Zhan, G., B. Oke, and C. Xya. 2020. \u201cA Multi-objective Differential Evolutionary Algorithm with Angle-based Objective Space Division and Parameter Adaption for Solving Sodium Gluconate Production Process and Benchmark Problems.\u201d Swarm and Evolutionary Computation 55: 100670, https:\/\/doi.org\/10.1016\/j.swevo.2020.100670.","DOI":"10.1016\/j.swevo.2020.100670"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_042","doi-asserted-by":"crossref","unstructured":"Zhao, B., L. Zhou, L. Ma, Y. He, J. Gao, D. Li, and Y. Jiang. 2018. \u201cCo-immobilization of Glucose Oxidase and Catalase in Silica Inverse Opals for Glucose Removal from Commercial Isomaltooligosaccharide.\u201d International Journal of Biological Macromolecules 107: 2034\u201343, https:\/\/doi.org\/10.1016\/j.ijbiomac.2017.10.074.","DOI":"10.1016\/j.ijbiomac.2017.10.074"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_043","doi-asserted-by":"crossref","unstructured":"Zhao, L., X. Kuang, C. Chen, X. Sun, Z. Wang, and Q. Wei. 2019. \u201cBoosting Electrocatalytic Nitrogen Fixation via Energy-efficient Anodic Oxidation of Sodium Gluconate.\u201d Chemical Communications 55: 10170\u20133, https:\/\/doi.org\/10.1039\/C9CC04378K.","DOI":"10.1039\/C9CC04378K"},{"key":"2025121207412232718_j_ijcre-2021-0237_ref_044","doi-asserted-by":"crossref","unstructured":"Zhuang, W., J. Huang, X. Liu, L. Ge, H. Niu, Z. Wang, J. Wu, P. Yang, Y. Chen, and H. Ying. 2019. \u201cCo-localization of Glucose Oxidase and Catalase Enabled by a Self-assembly Approach: Matching between Molecular Dimensions and Hierarchical Pore Sizes.\u201d Food Chemistry 275 (1): 197\u2013205, https:\/\/doi.org\/10.1016\/j.foodchem.2018.09.077.","DOI":"10.1016\/j.foodchem.2018.09.077"}],"container-title":["International Journal of Chemical Reactor Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/ijcre-2021-0237\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/ijcre-2021-0237\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T09:19:11Z","timestamp":1765531151000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/ijcre-2021-0237\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,7]]},"references-count":44,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2022,3,16]]},"published-print":{"date-parts":[[2022,9,19]]}},"alternative-id":["10.1515\/ijcre-2021-0237"],"URL":"https:\/\/doi.org\/10.1515\/ijcre-2021-0237","relation":{},"ISSN":["1542-6580"],"issn-type":[{"value":"1542-6580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,7]]}}}