{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T21:21:04Z","timestamp":1780435264457,"version":"3.54.1"},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>\n                  <jats:italic>N\u2032<\/jats:italic>-Nitrosodiethylamine (NDEA) is an effective hepatotoxicant, carcinogen and mutagen. NDEA-induced hepatic necrosis, through metabolic activation by CYP2E1, is an extensively used experimental model. In the present study, we analysed the dose- and time-dependent effect of NDEA on hepatic damage, RBC rheology and proteomic profile in male Wistar rats. The rats, 5\u20136 weeks old, were divided into four groups: Group-1 served as control and received normal saline, Group-2 received a single dose of 200 mg\/kg body weight NDEA intraperitoneally (i.p.) and the animals were sacrificed after one week; the rats of Group-3 received a single dose of 100 mg\/kg body weight NDEA and were sacrificed after one week; Group-4 received 100 mg\/kg body weight\/wk NDEA for two weeks and were then sacrificed. Various biochemical parameters such as ALT, AST, ALP and bilirubin were determined. Further, RBC rheology, histopathology (H&amp;E staining) of liver biopsies and polypeptide profiling (SDS-PAGE) in sera and liver sections were also carried out both in control and NDEA treated groups. Our results showed a significant increase in all the biochemical parameters of the liver function test (<jats:italic>p<\/jats:italic>&lt;0.05). In NDEA treated categories dacryocytes (tear drop cells), schistocytes (fragmented cells), codocytes (target cells), acanthocytes (spur cells) and ovalocytes (oval cells) were observed. H &amp; E stained liver biopsies treated with NDEA showed abnormal liver architecture with severe haemorrhage, neutrophilic infiltration and dysplastic hepatocytes manifested in a dose-dependent manner. Software analysis of SDS-PAGE of control and NDEA treated rat sera and liver revealed qualitative and quantitative differences in polypeptide composition. Based on the presence\/absence, polypeptides were classified in three different categories: (1) house-keeping, present in all the groups investigated; (2) novel, present in either control or NDEA treated group at any given time; (3) differential expression, showing quantitative differences. Our study indicates a dose and time-dependent hepatocellular damage and proteome profile which is likely due to NDEA-mediated oxidative stress in rats.<\/jats:p>","DOI":"10.1515\/intox-2015-0001","type":"journal-article","created":{"date-parts":[[2015,12,14]],"date-time":"2015-12-14T17:01:46Z","timestamp":1450112506000},"page":"1-7","source":"Crossref","is-referenced-by-count":25,"title":["Dose-dependent effect of <i>N\u2032<\/i>-Nitrosodiethylamine on hepatic architecture, RBC rheology and polypeptide repertoire in Wistar rats"],"prefix":"10.1515","volume":"8","author":[{"given":"Devoshree","family":"Mukherjee","sequence":"first","affiliation":[{"name":"Biochemical and Clinical Genetics Lab, Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh-202002 (U.P), India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riaz","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Biochemical and Clinical Genetics Lab, Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh-202002 (U.P), India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2015,12,10]]},"container-title":["Interdisciplinary Toxicology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/content.sciendo.com\/view\/journals\/intox\/8\/1\/article-p1.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.sciendo.com\/article\/10.1515\/intox-2015-0001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,6]],"date-time":"2021-04-06T21:59:51Z","timestamp":1617746391000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.sciendo.com\/article\/10.1515\/intox-2015-0001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,1]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2015,12,10]]},"published-print":{"date-parts":[[2015,3,1]]}},"alternative-id":["10.1515\/intox-2015-0001"],"URL":"https:\/\/doi.org\/10.1515\/intox-2015-0001","relation":{},"ISSN":["1337-9569"],"issn-type":[{"value":"1337-9569","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,1]]}}}