{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:09:33Z","timestamp":1787022573498,"version":"build-2736575974"},"reference-count":68,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2018,4,20]],"date-time":"2018-04-20T00:00:00Z","timestamp":1524182400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,4,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_iss-2018-0003_s_999_w2aab3b7c32b1b6b1aab1c17b1Aa\">\n                    <jats:title>Introduction:<\/jats:title>\n                    <jats:p>Lymphoedema is a worldwide pandemic causing swelling of tissues due to dysfunctional transport of lymph fluid. Present management concepts are based in conservative palliation of symptoms through manual lymphatic drainage, use of compression garments, manual lymph drainage, exercise, and skin care. Nevertheless, some curative options as autologous lymph node transplantation were shown to reduce lymphoedema in selected cases. Lately, some concern has arisen due to reports of donor site morbidity. A possible solution could be the development of artificial lymph node scaffolds as niches of lymphatic regeneration. Engineering these scaffolds has included cryopreservation of lymph node stroma. However, the effects of cryopreservation on the regeneration capacities of these organs were unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_iss-2018-0003_s_998_w2aab3b7c32b1b6b1aab1c17b2Aa\">\n                    <jats:title>Materials and methods:<\/jats:title>\n                    <jats:p>Here, we used the minipig animal model to assess lymphatic regeneration processes after cryopreservation of autologous lymph nodes. Superficial inguinal lymph nodes were excised and conserved at \u221280\u00b0C for 1 month. Thereafter, lymph node fragments were transplanted in the subcutaneous tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_iss-2018-0003_s_997_w2aab3b7c32b1b6b1aab1c17b3Aa\">\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Regeneration of the lymph nodes was assessed five months after transplantation. We show that lymph node fragment regeneration takes place in spite of former cryopreservation. Transplanted fragments presented typical histological appearance. Their draining capacity was documented by macroscopic transport of Berlin Blue dye as well as through SPECT-CT hybrid imaging.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_iss-2018-0003_s_996_w2aab3b7c32b1b6b1aab1c17b4Aa\">\n                    <jats:title>Discussion:<\/jats:title>\n                    <jats:p>In conclusion, our results suggest that processes of cryopreservation can be used in the creation of artificial lymph node scaffolds without major impairment of lymph node fragments regeneration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/iss-2018-0003","type":"journal-article","created":{"date-parts":[[2018,4,20]],"date-time":"2018-04-20T18:16:17Z","timestamp":1524248177000},"page":"139-146","source":"Crossref","is-referenced-by-count":2,"title":["Effect of cryopreservation on lymph node fragment regeneration after autologous transplantation in the minipig model"],"prefix":"10.1515","volume":"3","author":[{"given":"Catarina","family":"Hadamitzky","sequence":"first","affiliation":[{"name":"Practice for Lympho-Vascular Diseases Bahnhofstrasse 12, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanes","family":"Peri\u0107","sequence":"additional","affiliation":[{"name":"myDent GmbH Tiergartenstrasse 114, 30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian J.","family":"Theobald","sequence":"additional","affiliation":[{"name":"Clinic of Haematology, Haemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus Friedrich","family":"Gratz","sequence":"additional","affiliation":[{"name":"Clinic of Nuclear Medicine, Hannover Medical School, Hannover, 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