{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T20:14:12Z","timestamp":1760472852994,"version":"3.37.3"},"reference-count":35,"publisher":"Walter de Gruyter GmbH","issue":"1-2","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BU 1857\/2-1"],"award-info":[{"award-number":["BU 1857\/2-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p\/>\n               <jats:sec id=\"j_iss-2020-0017_abs_001\">\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Cutaneous ischemia\/reperfusion (CI\/R) injury has shown to play a significant role in chronic wounds such as decubitus ulcers, diabetic foot ulcers, atherosclerotic lesions, and venous stasis wounds. CI\/R also plays a role in free tissue transfer in reconstructive microsurgery and has been linked to clinical burn-depth progression after thermal injury. While the role of the complement system has been elucidated in multiple organ systems, evidence is lacking with respect to its role in the skin. Therefore, we evaluated the role of the complement system in CI\/R injury.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_iss-2020-0017_abs_002\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Using a single pedicle skin flap mouse model of acute CI\/R, we performed CI\/R in wild-type (WT) mice and complement knock out (KO) mice, deficient in either C1q (C1q KO; classical pathway inhibition), mannose-binding lectin (MBL null; lectin pathway inhibition) or factor B (H2Bf KO; alternative pathway inhibition). Following 10\u00a0h ischemia and 7 days reperfusion, mice were sacrificed, flaps harvested and flap viability assessed via Image J software. The flap necrotic area was expressed as % total flap area. In another group, mice were sacrificed following CI\/R with 10\u00a0h ischemia and 48\u00a0h reperfusion. Two cranial skin flap samples were taken for gene expression analysis of IL1b, IL6, TNF\u03b1, ICAM1, VCAM1, IL10, IL13 using real-time polymerase chain reaction (RT-PCR).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_iss-2020-0017_abs_003\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Following CI\/R, MBL null mice had a statistically significant smaller %necrotic flap area compared to WT mice (10.6 vs. 43.1%; p&lt;0.05) suggesting protection from CI\/R. A significantly reduced mean %necrotic flap area was not seen in either C1q KO or H2Bf KO mice relative to WT (22.9 and 31.3 vs. 43.1%; p=0.08 and p=0.244, respectively). There were no statistically significant differences between groups for markers of inflammation (TNF\u03b1, ICAM1, VCAM1, IL1b, IL6). In contrast, mRNA levels of IL10, a regulator of inflammation, were significantly increased in the MBL null group (p=0.047).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_iss-2020-0017_abs_004\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We demonstrated for the first time a significant role of MBL and the lectin complement pathway in ischemia\/reperfusion injury of the skin and a potential role for IL10 in attenuating CI\/R injury, as IL10 levels were significantly increased in the tissue from the CI\/R-protected MBL null group.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/iss-2020-0017","type":"journal-article","created":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T06:00:01Z","timestamp":1599804001000},"page":"43-51","source":"Crossref","is-referenced-by-count":3,"title":["Mannose-binding lectin (MBL) and the lectin complement pathway play a role in cutaneous ischemia and reperfusion injury"],"prefix":"10.1515","volume":"5","author":[{"given":"Claas-Tido","family":"Peck","sequence":"first","affiliation":[{"name":"Hannover Medical School , Department of Plastic, Aesthetic, Hand, and Reconstructive Surgery , Hannover , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Strau\u00df","sequence":"additional","affiliation":[{"name":"Hannover Medical School , Department of Plastic, Aesthetic, Hand, and Reconstructive Surgery , Hannover , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory L.","family":"Stahl","sequence":"additional","affiliation":[{"name":"Harvard Medical School , Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women\u2019s Hospital , Boston , MA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter-Maria","family":"Vogt","sequence":"additional","affiliation":[{"name":"Hannover Medical School , Department of Plastic, Aesthetic, Hand, and Reconstructive Surgery , Hannover , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3997-2216","authenticated-orcid":false,"given":"Marc N.","family":"Busche","sequence":"additional","affiliation":[{"name":"Hannover Medical School , Department of Plastic, Aesthetic, Hand, and Reconstructive Surgery , Hannover , Germany"},{"name":"Leverkusen Hospital gGmbH , Department of Plastic and Aesthetic Surgery, Burn Surgery , Leverkusen , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2020,9,14]]},"reference":[{"doi-asserted-by":"crossref","unstructured":"Riedemann, NC, Ward, PA. 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