{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T12:35:23Z","timestamp":1765542923733,"version":"3.48.0"},"reference-count":32,"publisher":"Walter de Gruyter GmbH","issue":"2","funder":[{"name":"Fundamental Research Grant SchemeMinistry of Higher Education","award":["FRGS\/1\/2016\/STG03\/UM\/02\/2"],"award-info":[{"award-number":["FRGS\/1\/2016\/STG03\/UM\/02\/2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_jbcpp-2020-0320_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Primary Osteoarthritis (OA) is a disease of progressive joints degeneration due to idiopathic causes. Recent evidence showed a positive relationship between OA and metabolic syndrome. This pilot study aimed to\u00a0assess the baseline level of pro and anti-inflammatory cytokines in OA patients with or without Diabetic Mellitus (DM) and assess the effect of hydrogen peroxide (H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      ) in cytokine production.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jbcpp-2020-0320_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Patients with primary hip and knee OA were recruited, and 3\u00a0mL of bone marrow was harvested during joint replacement surgery. Bone marrow stromal cells (BMSC) was isolated and cultured in a culture flask for three passages. Later experiment was then sub-cultured in a well plate labeled as the control group and H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      (0.1\u00a0mM) treated group. ProcartaPlex\n                      <jats:sup>\u00ae<\/jats:sup>\n                      Multiplex Immunoassay was performed to measure cytokine levels produced by the BMSC at 0\u00a0h, as well as 72\u00a0h.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jbcpp-2020-0320_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Cytokines such as tumor necrosis factor-alpha, interleukin (IL)-6, IL-8, and IL-1\u03b2 generally exhibited higher cytokine levels in subjects with DM than in nonDM subjects at 0 and 72\u00a0h. For IL-17, its expression was similar in nonDM and DM groups at 0 and 72\u00a0h. Cytokine IL-10 showed no significant difference in both the groups while DM and nonDM groups treated with H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      showed decreased IL-4 levels compared to control groups at 72\u00a0h. Bone marrow cells from DM-OA are more vulnerable to chemical insult and are associated with higher levels of proinflammatory cytokines production and lower IL-4 level production.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jbcpp-2020-0320_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study provides a clue that management of OA with co-morbidity like DM needs future studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/jbcpp-2020-0320","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T18:03:20Z","timestamp":1624903400000},"page":"177-185","source":"Crossref","is-referenced-by-count":1,"title":["Cytokine release by human bone marrow stromal cells isolated from osteoarthritic and diabetic osteoarthritic patients\n                    <i>in vitro<\/i>"],"prefix":"10.1515","volume":"34","author":[{"given":"Kar Wai","family":"Loh","sequence":"first","affiliation":[{"name":"Tissue Engineering Group, Department of Orthopaedic Surgery (NOCERAL), Faculty of Medicine , University of Malaya , Kuala Lumpur , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norshazliza","family":"Shaz","sequence":"additional","affiliation":[{"name":"Tissue Engineering Group, Department of Orthopaedic Surgery (NOCERAL), Faculty of Medicine , University of Malaya , Kuala Lumpur , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simmrat","family":"Singh","sequence":"additional","affiliation":[{"name":"Tissue Engineering Group, Department of Orthopaedic Surgery (NOCERAL), Faculty of Medicine , University of Malaya , Kuala Lumpur , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murali","family":"Malliga Raman","sequence":"additional","affiliation":[{"name":"Tissue Engineering Group, Department of Orthopaedic Surgery (NOCERAL), Faculty of Medicine , University of Malaya , Kuala Lumpur , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanumantha Rao","family":"Balaji Raghavendran","sequence":"additional","affiliation":[{"name":"Tissue Engineering Group, Department of Orthopaedic Surgery (NOCERAL), Faculty of Medicine , University of Malaya , Kuala Lumpur , Malaysia"},{"name":"Sri Ramachandra Institute of Higher Education and Research , Chennai , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tunku","family":"Kamarul","sequence":"additional","affiliation":[{"name":"Tissue Engineering Group, Department of Orthopaedic Surgery (NOCERAL), Faculty of Medicine , University of Malaya , Kuala Lumpur , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2021,6,28]]},"reference":[{"key":"2025121209291592850_j_jbcpp-2020-0320_ref_001","unstructured":"Felson, D. 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