{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T11:35:31Z","timestamp":1765539331660,"version":"3.48.0"},"reference-count":39,"publisher":"Walter de Gruyter GmbH","issue":"4","funder":[{"name":"Directorate General of Higher Education through World Class Research","award":["945\/UN3.14\/PT\/2021"],"award-info":[{"award-number":["945\/UN3.14\/PT\/2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_jbcpp-2020-0414_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      The rapid spread of antimalarial drug resistance is becoming a problem in the treatment of malaria. The fact was indicated the importance of finding new antimalarial drugs. The genus Garcinia is well known to be a rich source of bioactive prenylated xanthones and triterpenes reported for their antimalarial activity.\n                      <jats:italic>Garcinia parvifolia<\/jats:italic>\n                      is one of the Garcinia genera that can be explored for the search of new antimalarial drugs. This study was aimed to determine the antimalarial activities of\n                      <jats:italic>G.\u00a0parvifolia<\/jats:italic>\n                      extracts and fractions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jbcpp-2020-0414_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Garcinia parvifolia<\/jats:italic>\n                      Miq. stem was collected from\u00a0Balikpapan Botanical Garden in East Kalimantan, Indonesia, was extracted gradually with n-hexane, dichloromethane, and methanol by ultrasonic assisted method. The most active extract was further separated using the open column chromatography method. All extracts and fractions were tested against\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      3D7 using lactate dehydrogenase (LDH) assay and followed by IC\n                      <jats:sub>50<\/jats:sub>\n                      determination.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jbcpp-2020-0414_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results showed that all extracts inhibit\n                      <jats:italic>P.\u00a0falciparum<\/jats:italic>\n                      growth by LDH assay. The highest inhibition was showed by dichloromethane stem extract (BP12-S-D) with the IC\n                      <jats:sub>50<\/jats:sub>\n                      value of 6.61\u00a0\u00b1\u00a00.09\u00a0\u03bcg\/mL. Further fractionation of BP12-S-D has obtained 10 fractions. All of them\u00a0were identified by TLC, and a brownish-yellow spot (fraction-1) appears after spraying with 10% H\n                      <jats:sub>2<\/jats:sub>\n                      SO\n                      <jats:sub>4<\/jats:sub>\n                      . Fraction-1 (F1) performed the highest parasite growth inhibition with the IC\n                      <jats:sub>50<\/jats:sub>\n                      value of 6.00\u00a0\u00b1\u00a00.03\u00a0\u03bcg\/mL compared with other fractions. This fraction was classified as having a promising activity of antimalarial. The fraction-1 was identified using HPLC, and two major peaks were observed (A and B). The UV\u2013Vis spectra showed the absorption at wavelengths 250 and 278 (A), 243, 281, and 317\u00a0nm (B). Based on the profile of TLC, HPLC, and UV\u2013Vis spectra of F1, it was expected that the active compounds are flavonoid (A)\u00a0and xanthone (B).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jbcpp-2020-0414_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The fraction-1 of dichloromethane extract of\n                      <jats:italic>G.\u00a0parvifolia<\/jats:italic>\n                      Miq. stem has the highest antimalarial activity. It might be a potential candidate for the new antimalarial drug.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/jbcpp-2020-0414","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T12:45:59Z","timestamp":1625229959000},"page":"839-844","source":"Crossref","is-referenced-by-count":4,"title":["<i>In vitro<\/i>\n                    antimalarial activity of\n                    <i>Garcinia parvifolia<\/i>\n                    Miq. Stem extracts and fractions on\n                    <i>Plasmodium falciparum<\/i>\n                    lactate dehydrogenase (LDH) assay"],"prefix":"10.1515","volume":"32","author":[{"given":"Marsih","family":"Wijayanti","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy , Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hilkatul","family":"Ilmi","sequence":"additional","affiliation":[{"name":"Center of Natural Product Medicine Research and Development , Institute of Tropical Disease, Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Einstenia","family":"Kemalahayati","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy , Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lidya","family":"Tumewu","sequence":"additional","affiliation":[{"name":"Center of Natural Product Medicine Research and Development , Institute of Tropical Disease, Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fendi Yoga","family":"Wardana","sequence":"additional","affiliation":[{"name":"Center of Natural Product Medicine Research and Development , Institute of Tropical Disease, Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Suciati","sequence":"additional","affiliation":[{"name":"Center of Natural Product Medicine Research and Development , Institute of Tropical Disease, Universitas Airlangga , Surabaya , Indonesia"},{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy , Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Achmad Fuad","family":"Hafid","sequence":"additional","affiliation":[{"name":"Center of Natural Product Medicine Research and Development , Institute of Tropical Disease, Universitas Airlangga , Surabaya , Indonesia"},{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy , Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aty","family":"Widyawaruyanti","sequence":"additional","affiliation":[{"name":"Center of Natural Product Medicine Research and Development , Institute of Tropical Disease, Universitas Airlangga , Surabaya , Indonesia"},{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy , Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2021,6,25]]},"reference":[{"key":"2025121209024983368_j_jbcpp-2020-0414_ref_001","unstructured":"World Health Organization. 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