{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T11:32:07Z","timestamp":1765539127281,"version":"3.48.0"},"reference-count":18,"publisher":"Walter de Gruyter GmbH","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,25]]},"abstract":"Abstract<\/jats:title>\n \n Objectives<\/jats:title>\n \n Escherichia coli<\/jats:italic>\n is one of the pathogenic bacteria that caused a nosocomial infection. Levofloxacin is one of the fluoroquinolones group antibiotics which is a broad-spectrum antibiotic that works effectively against\n E.\u00a0coli<\/jats:italic>\n . The mutation can happen in the bacteria which caused a resistant effect in the use of antibacterial. This study aimed at identifying mutation in gene\n gyrA<\/jats:italic>\n among\n E.\u00a0coli<\/jats:italic>\n that were resistant to levofloxacin.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The susceptibility of\n E<\/jats:italic>\n . coli<\/jats:italic>\n was determined by disk diffusion. PCR and sequencing were performed to identify the mutation in\n gyrA<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n A total of 10 isolates showed result resistance to levofloxacin and gyrA gene mutation in the amino acid changes. Nucleotide sequence analysis revealed a point mutation in QRDR (quinolone resistance determining region) of gyrA Ser83\u2192Leu, Asp87\u2192Asn. The silent mutation was also found at codon Val85, Arg91, Ser111, Thr123.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Mutation in the\n gyrA<\/jats:italic>\n gene affect the occurrence of bacterial resistance of\n E.\u00a0coli<\/jats:italic>\n to levofloxacin.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1515\/jbcpp-2020-0445","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T12:45:59Z","timestamp":1625229959000},"page":"751-754","source":"Crossref","is-referenced-by-count":1,"title":["Profile of gyrA gene mutation in clinical isolate of levofloxacin resistant\n Escherichia coli<\/i>"],"prefix":"10.1515","volume":"32","author":[{"given":"Alifia Risma","family":"Fahmi","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Universitas Airlngga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Suharjono","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy , Faculty of Pharmacy, Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Kuntaman","sequence":"additional","affiliation":[{"name":"Departement of Clinical Microbiology , Faculty of Medcine, Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2021,6,25]]},"reference":[{"key":"2025121209024973863_j_jbcpp-2020-0445_ref_001","doi-asserted-by":"crossref","unstructured":"Revelas, A. 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