{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T11:22:58Z","timestamp":1765538578016,"version":"3.48.0"},"reference-count":14,"publisher":"Walter de Gruyter GmbH","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,25]]},"abstract":"Abstract<\/jats:title>\n \n Objectives<\/jats:title>\n \n The purpose of this study is to use the second internal transcribed spacer (ITS-2) to determine the molecular characteristics of\n Sarcoptes scabiei<\/jats:italic>\n in rabbits from several areas of East Java.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Collecting\n S.\u00a0scabiei<\/jats:italic>\n mites from rabbits with clinical signs of scabies; DNA extraction with minikit QIAamp DNA; polymerase chain reaction amplification; nucleotide sequence analysis; homology and phylogenetic tree using the Neighbor-Joining method in the program molecular evolutionary genetics analysis-7 (MEGA-7).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Sequence analysis of ITS-2\n S.\u00a0scabiei<\/jats:italic>\n from five regions in East Java showed an identity >91.23% with isolates from China (KX695125.1). The phylogenetic analysis of ITS-2\n S.\u00a0scabiei<\/jats:italic>\n from Mojokerto rabbits has a close relationship with AB82977.1; Surabaya and Nganjuk rabbits are closely related to KX695125.1; while Sidoarjo and Pasuruan rabbits are closely related to EF514469.2. and AB369384.1.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n The homology analysis of all samples showed identity of more than 91.23% with isolate China (KX695125.1). The sequences of ITS-2 gen of\n S.\u00a0scabiei<\/jats:italic>\n from rabbits in several areas were relatively close to\n S.\u00a0scabiei<\/jats:italic>\n obtain various hosts from National Centre for Biotechnology Information (NCBI) data.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1515\/jbcpp-2020-0467","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T14:31:11Z","timestamp":1625236271000},"page":"701-705","source":"Crossref","is-referenced-by-count":0,"title":["Second internal transcribed spacer (ITS-2) as genetic marker for molecular characterization of\n Sarcoptes scabiei<\/i>\n in rabbits from several areas of East Java, Indonesia"],"prefix":"10.1515","volume":"32","author":[{"given":"Nunuk Dyah Retno","family":"Lastuti","sequence":"first","affiliation":[{"name":"Department of Veterinary Parasitology, Faculty of Veterinary Medicine , Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nur","family":"Rusdiana","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine , Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poedji","family":"Hastutiek","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, Faculty of Veterinary Medicine , Universitas Airlangga , Surabaya , Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2021,6,25]]},"reference":[{"key":"2025121208571263564_j_jbcpp-2020-0467_ref_001","doi-asserted-by":"crossref","unstructured":"Romani, L, Steer, AC, Whitfeld, MJ, Kaldor, JM. 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