{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T12:12:11Z","timestamp":1765541531963,"version":"3.48.0"},"reference-count":34,"publisher":"Walter de Gruyter GmbH","issue":"1-2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_jbcpp-2023-0034_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>How gaseous signalling molecules affect ion transport processes contributing to the physiological functions of the gastrointestinal tract under hypoxic conditions still needs to be clarified. The objective of the present study was to characterize the impact of gaseous signalling molecules on parameters of colonic ion transport during a hypoxia\/reoxygenation cycle and the remaining secretory capacity of the epithelium after such a cycle.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jbcpp-2023-0034_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Short-circuit current (I\n                      <jats:sub>sc<\/jats:sub>\n                      ) and tissue conductance (G\n                      <jats:sub>t<\/jats:sub>\n                      ) recordings in Ussing chamber experiments were performed on rat colon samples using CORM-2 (putative CO donor; 35 and 350\u202f\u00b5M), sodium nitroprusside (NO donor; 100\u202f\u00b5M), NaHS (fast H\n                      <jats:sub>2<\/jats:sub>\n                      S donor; 10\u00a0\u2013 1,000\u202f\u00b5M), GYY 4137 (slow\u00a0H\n                      <jats:sub>2<\/jats:sub>\n                      S donor; 50\u202f\u00b5M) and Angeli\u2019s salt (HNO donor; 100\u202f\u00b5M) as donors for gasotransmitters. Inhibition of endogenous synthesis of H\n                      <jats:sub>2<\/jats:sub>\n                      S was operated by inhibitors of cystathionin-\u03b3-lyase, i.e.\u00a0\n                      <jats:sc>dl<\/jats:sc>\n                      -propargylglycine (1\u202fmM) or \u03b2-cyano-\n                      <jats:sc>l<\/jats:sc>\n                      -alanine (5\u202fmM), and the inhibitor of cystathionine-\u03b2-synthase, amino-oxyacetate (5\u202fmM).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jbcpp-2023-0034_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The fast gasotransmitter donors NaHS, sodium nitroprusside and Angeli\u2019s salt, administered 5\u202fmin before the onset of hypoxia, induced an increase in I\n                      <jats:sub>sc<\/jats:sub>\n                      . The response to the subsequently applied hypoxia was characterized by a decrease in I\n                      <jats:sub>sc<\/jats:sub>\n                      , which tended to be reduced only in the presence of the lowest concentration of NaHS (10\u202f\u00b5M) tested. Reoxygenation resulted in a slow increase in I\n                      <jats:sub>sc<\/jats:sub>\n                      , which was unaffected by all donors or inhibitors tested. The stable acetylcholine derivative carbachol (50\u202f\u00b5M) was administered at the end of each hypoxia\/reoxygenation cycle to test the secretory capacity of the epithelium. Pretreatment of the\u00a0tissue with the putative CO donor CORM-2 suppressed the\u00a0secretory response induced by carbachol. The same was\u00a0observed when cystathionin-\u03b3-lyase and cystathionin-\u03b3-synthase were inhibited simultaneously. Under both conditions, G\n                      <jats:sub>t<\/jats:sub>\n                      drastically increased suggesting an impaired tissue integrity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jbcpp-2023-0034_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The present results demonstrate that none of the exogenous gasotransmitter releasing drugs significantly ameliorated the changes in epithelial ion transport during the hypoxia\/reoxygenation cycle ex\u00a0vivo. In contrast, the putative CO donor CORM-2 exerted a toxic effect on the epithelium. The endogenous production of H\n                      <jats:sub>2<\/jats:sub>\n                      S, however, seems to have a protective effect on the mucosal integrity and the epithelial transport functions, which\u00a0\u2013 when inhibited\u00a0\u2013 leads to a loss of the secretory ability of the mucosa. This observation together with the trend for improvement observed with a low concentration of the H\n                      <jats:sub>2<\/jats:sub>\n                      S donor NaHS suggests a moderate protective role of low concentrations of H\n                      <jats:sub>2<\/jats:sub>\n                      S under hypoxic conditions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/jbcpp-2023-0034","type":"journal-article","created":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:30:45Z","timestamp":1705969845000},"page":"61-70","source":"Crossref","is-referenced-by-count":1,"title":["Gasotransmitters do not prevent changes in transepithelial ion transport induced by hypoxia followed by reoxygenation"],"prefix":"10.1515","volume":"35","author":[{"given":"Rebecca","family":"Cla\u00dfen","sequence":"first","affiliation":[{"name":"Institute for Veterinary Physiology and Biochemistry , Justus-Liebig-Universitat Giessen , Giessen , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Diener","sequence":"additional","affiliation":[{"name":"Institute for Veterinary Physiology and Biochemistry , Justus-Liebig-Universitat Giessen , Giessen , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ervice","family":"Pouokam","sequence":"additional","affiliation":[{"name":"Institute for Veterinary Physiology and Biochemistry , Justus-Liebig-Universitat Giessen , Giessen , Germany"},{"name":"Department of Human Medicine, MSB Medical School Berlin , R\u00fcdesheimer Str. 50 , 14197 Berlin , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2024,1,23]]},"reference":[{"key":"2025121209194136578_j_jbcpp-2023-0034_ref_001","unstructured":"Binder, HJ, Sandle, GI. 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