{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T02:20:23Z","timestamp":1773454823959,"version":"3.50.1"},"reference-count":35,"publisher":"Walter de Gruyter GmbH","funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006144","name":"Kerala State Council for Science, Technology and Environment","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006144","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_jbcpp-2024-0042_abs_001\">\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Type 2 diabetes (T2DM) and alcoholism are considered to be lifestyle-associated independent risk factors in fatty liver diseases (FLD) mediated cirrhosis and hepatocellular carcinoma (HCC). A combined effect of both these conditions may exacerbate the pathological changes and a pre-clinical exploration of this is expected to provide a mechanical detail of the pathophysiology. The present study aims to understand the effect of alcohol on pre- diabetic and type 2 diabetic female Wistar rats.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jbcpp-2024-0042_abs_002\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>In this experimental study, 12 Wistar rats (180\u2013220\u202fg) were randomly assigned into three groups: Normal (fed normal rat chow), alcohol (20\u202f%) fed diabetic (HFD\u00a0+\u00a0STZ), and pre-diabetic rats (HFD alone). After, two months of the experimental period, blood and liver tissues were collected lipid metabolic alteration, liver injury, and fibrosis were determined following biochemical and histological methods. Data were analyzed using one-way ANOVA and Dunnett\u2019s Post Hoc test.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jbcpp-2024-0042_abs_003\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Significant dyslipidemia was observed in the liver tissues of diabetic and pre-diabetic rats following alcohol ingestion. A significant (p&lt;0.05) increase in lipid peroxidation status, and hepatic marker enzyme activities (p&lt;0.0001) were observed in diabetic animals. In corroborating with these observations, hematoxylin and eosin staining of hepatic tissue revealed the presence of sinusoidal dilation along with heavily damaged hepatocytes and inflammatory cell infiltration. Further, significantly (p&lt;0.001) increased hepatic hydroxyproline content and extended picrosirius red stained areas of collagen in liver tissue indicated initiation of fibrosis in alcohol-fed diabetic rats.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jbcpp-2024-0042_abs_004\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Overall, the results indicate that alcohol consumption in T2DM conditions is more deleterious than pre diabetic conditions in progressing to hepatic fibrosis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/jbcpp-2024-0042","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T15:32:16Z","timestamp":1721316736000},"source":"Crossref","is-referenced-by-count":2,"title":["Alcohol promotes liver fibrosis in high fat diet induced diabetic rats"],"prefix":"10.1515","author":[{"given":"Veena","family":"Gopinath","sequence":"first","affiliation":[{"name":"Department of Biochemistry , Amala Cancer Research Center (Recognized Centre of the University of Calicut) , Thrissur , Kerala , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleena","family":"Mariya Davis","sequence":"additional","affiliation":[{"name":"Department of Biochemistry , Amala Cancer Research Center (Recognized Centre of the University of Calicut) , Thrissur , Kerala , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thara K.","family":"Menon","sequence":"additional","affiliation":[{"name":"Department of Biotechnology , University of Calicut , Thenhipalam , Kerala , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8760-359X","authenticated-orcid":false,"given":"Achuthan C.","family":"Raghavamenon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry , Amala Cancer Research Center (Recognized Centre of the University of Calicut) , Thrissur , Kerala , India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2024,7,19]]},"reference":[{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_001","doi-asserted-by":"crossref","unstructured":"Lieber, CS. Hepatic, metabolic and toxic effects of ethanol. Alcohol Clin Exp Res 1991;15:573\u201392. https:\/\/doi.org\/10.1111\/j.1530-0277.1991.tb00563.x.","DOI":"10.1111\/j.1530-0277.1991.tb00563.x"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_002","doi-asserted-by":"crossref","unstructured":"Lebovics, E, Rubin, J. Non-alcoholic fatty liver disease (NAFLD): why you should care, when you should worry, what you should do. Diabetes Metab Res Rev 2011;27:419\u201324. https:\/\/doi.org\/10.1002\/dmrr.1198.","DOI":"10.1002\/dmrr.1198"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_003","doi-asserted-by":"crossref","unstructured":"Wan, X, Xu, C, Yu, C, Li, Y. Role of NLRP3 inflammasome in the progression of NAFLD to NASH. 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Free Radic Biol Med 2012;52:59\u201369. https:\/\/doi.org\/10.1016\/j.freeradbiomed.2011.10.003.","DOI":"10.1016\/j.freeradbiomed.2011.10.003"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_006","doi-asserted-by":"crossref","unstructured":"Serviddio, G, Bellanti, F, Vendemiale, G. Free radical biology for medicine: learning from nonalcoholic fatty liver disease. Free Radic Biol Med 2013;65:952\u201368. https:\/\/doi.org\/10.1016\/j.freeradbiomed.2013.08.174.","DOI":"10.1016\/j.freeradbiomed.2013.08.174"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_007","doi-asserted-by":"crossref","unstructured":"Gonzalez, A, Huerta-Salgado, C, Orozco-Aguilar, J, Aguirre, F, Tacchi, F, Simon, F, et al.. Role of oxidative stress in hepatic and extrahepatic dysfunctions during Nonalcoholic Fatty Liver Disease (NAFLD). Oxid Med Cell Longev 2020;19:1617805. https:\/\/doi.org\/10.1155\/2020\/1617805.","DOI":"10.1155\/2020\/1617805"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_008","doi-asserted-by":"crossref","unstructured":"Hassan, MM, Hwang, LY, Hatten, CJ, Swaim, M, Li, D, Abbruzzese, JL, et al.. Risk factors for hepatocellular carcinoma: synergism of alcohol with viral hepatitis and diabetes mellitus. Hepatology 2002;36:1206\u201313. https:\/\/doi.org\/10.1053\/jhep.2002.36780.","DOI":"10.1053\/jhep.2002.36780"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_009","doi-asserted-by":"crossref","unstructured":"Yuan, JM, Govindarajan, S, Arakawa, K, Yu, MC. Synergism of alcohol, diabetes, and viral hepatitis on the risk of hepatocellular carcinoma in blacks and whites in the U.S. Cancer 2004;101:1009\u201317. https:\/\/doi.org\/10.1002\/cncr.20427.","DOI":"10.1002\/cncr.20427"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_010","doi-asserted-by":"crossref","unstructured":"Saito, K, Uebanso, T, Maekawa, K, Ishikawa, M, Taguchi, R, Nammo, T, et al.. Characterization of hepatic lipid profiles in a mouse model with nonalcoholic steatohepatitis and subsequent fibrosis. Sci Rep 2015;5:12466. https:\/\/doi.org\/10.1038\/srep12466.","DOI":"10.1038\/srep12466"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_011","doi-asserted-by":"crossref","unstructured":"Gopinath, V, Shamsitha, MKA, Penarveettil Nair, V, Seena, P, Uppu, RM, Raghavamenon, AC. Thermally oxidized coconut oil as fat source in high-fat diet induces hepatic fibrosis in diabetic rat model. 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Alcohol Clin Exp Res 2007;31:1746\u201358. https:\/\/doi.org\/10.1111\/j.1530-0277.2007.00478.x.","DOI":"10.1111\/j.1530-0277.2007.00478.x"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_014","doi-asserted-by":"crossref","unstructured":"Friedewald, WT, Levy, RI, Fredrickson, DS. Estimation of the concentration of low-density lipoproteincholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18:499\u2013502. https:\/\/doi.org\/10.1093\/clinchem\/18.6.499.","DOI":"10.1093\/clinchem\/18.6.499"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_015","doi-asserted-by":"crossref","unstructured":"Folch, J, Lees, M, Stanley, CHS. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 1957;226:497\u2013509. https:\/\/doi.org\/10.1016\/s0021-9258(18)64849-5.","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_016","unstructured":"Zlatkis, A, Zak, B, Boyle, AJ. 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J Biol Chem 1996;313:893\u20138. https:\/\/doi.org\/10.1042\/bj3130893.","DOI":"10.1042\/bj3130893"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_028","doi-asserted-by":"crossref","unstructured":"Dilworth, L, Facey, A, Omoruyi, F. Diabetes mellitus and its metabolic complications: the role of adipose tissues. Int J Mol Sci 2021;22:7644. https:\/\/doi.org\/10.3390\/ijms22147644.","DOI":"10.3390\/ijms22147644"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_029","doi-asserted-by":"crossref","unstructured":"Abel, T, Feh\u00e9r, J. A m\u00e9rs\u00e9kelt alkoholfogyaszt\u00e1s hat\u00e1sa az inzulin\u00e9rz\u00e9kenys\u00e9gre [Effect of moderate alcohol consumption on insulin sensitivity]. Orv Hetil 2009;150:2218\u201321. https:\/\/doi.org\/10.1556\/OH.2009.28750.","DOI":"10.1556\/oh.2009.28750"},{"key":"2024082614405699407_j_jbcpp-2024-0042_ref_030","doi-asserted-by":"crossref","unstructured":"Risti\u0107-Medi\u0107, D, Risti\u0107, G, Tepsi\u0107, V, Risti\u0107, GN. 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