{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:45:55Z","timestamp":1791960355524,"version":"4.3.4"},"reference-count":28,"publisher":"Walter de Gruyter GmbH","issue":"2","funder":[{"name":"Anhui Province clinical medical research transformation project","award":["202427b10020009"],"award-info":[{"award-number":["202427b10020009"]}]},{"name":"Xinglin talent training program","award":["0500-48-30"],"award-info":[{"award-number":["0500-48-30"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_jcim-2024-0435_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This investigation aimed to evaluate the efficacy, key active ingredients, and mechanisms of Zuoqing San (ZQS), a traditional Chinese medicine formula, in managing sigmoid ulcerative colitis (SUC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2024-0435_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A cohort of 126 participants was recruited and treated with ZQS at a daily dosage of 100\u202fmL for 12\u00a0weeks. The primary endpoint was the percentage of subjects achieving favorable treatment outcomes. Liquid chromatography-mass spectrometry was employed to identify the principal ingredients of ZQS. Network pharmacology was utilized to predict the central targets of its active ingredients. Protein-protein interaction networks, Gene Ontology enrichment analyses, and Kyoto Encyclopedia of Genes and Genomes pathway analyses were conducted for identified core targets. Finally, molecular dynamics simulations of key active ingredients and core targets were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2024-0435_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Following 12\u00a0weeks of therapy, with a withdrawal rate of 7.93\u202f%, favorable treatment outcomes were observed in 31.03\u202f% of subjects at 4\u00a0weeks, 66.37\u202f% at 8\u00a0weeks (Odds Ratio: 1.54, 95\u202f% Confidence Interval: 0.41\u20131.83), and 68.10\u202f% at 12\u00a0weeks (Odds Ratio: 1.86, 95\u202f% CI: 0.32\u20131.27). ZQS comprises 31 principal chemical constituents. Key targets within the protein-protein interaction network included TNF, AKT1, IL6, IL1\u03b2, PTGS2, TP53, JUN, MMP9, CASP3, HIF1A, and BCL1. Pathway analysis indicated ZQS primarily impacts the TNF, NF-kB, and IL-17 signaling pathways. Molecular dynamics simulation results showed oxymatrine, cynarin had higher affinity with TNF and IL1\u03b2, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2024-0435_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This research elucidates the active components of ZQS and its potential multicomponent\u2013multitarget\u2013multipathway pharmacological mechanisms, demonstrating promising therapeutic potential for SUC management.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/jcim-2024-0435","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T16:47:03Z","timestamp":1740156423000},"page":"343-352","source":"Crossref","is-referenced-by-count":3,"title":["The active ingredients and mechanism of Zuoqing San in the treatment of sigmoid ulcerative colitis by retention enema"],"prefix":"10.1515","volume":"22","author":[{"given":"Ming","family":"Li","sequence":"first","affiliation":[{"name":"Department of Anorectal Surgery , 117843 The First Affiliated Hospital of Anhui University of Chinese Medicine , Hefei , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Anorectal Surgery , 117843 The Second Affiliated Hospital of Anhui University of Chinese Medicine , Hefei , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqing","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Anorectal Surgery , 117843 The First Affiliated Hospital of Anhui University of Chinese Medicine , Hefei , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Anorectal Surgery , 117843 The First Affiliated Hospital of Anhui University of Chinese Medicine , Hefei , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Anorectal Surgery , 117843 The First Affiliated Hospital of Anhui University of Chinese Medicine , Hefei , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Anorectal Surgery , 117843 The First Affiliated Hospital of Anhui University of Chinese Medicine , Hefei , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ansheng","family":"Zha","sequence":"additional","affiliation":[{"name":"Gastroenterology Department , The First Affiliated Hospital of Anhui University of Chinese Medicine , Hefei , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,2,24]]},"reference":[{"key":"2026101405585243690_j_jcim-2024-0435_ref_001","doi-asserted-by":"crossref","unstructured":"Wang, YJ, Li, QM, Zha, XQ, Luo, JP. 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