{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T12:44:46Z","timestamp":1782650686971,"version":"3.54.5"},"reference-count":72,"publisher":"Walter de Gruyter GmbH","issue":"4","funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"crossref","award":["E\u221226\/201.373\/2021"],"award-info":[{"award-number":["E\u221226\/201.373\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES) \u2013 Brazil","award":["Code 001"],"award-info":[{"award-number":["Code 001"]}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["2022\/14824-4"],"award-info":[{"award-number":["2022\/14824-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_jcim-2025-0103_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Milk products are good vehicles for probiotics due to their physical-chemical characteristics, improving probiotic survival in food and in the gastrointestinal tract.\n                      <jats:italic>Lactobacillus acidophilus<\/jats:italic>\n                      La-05 is known to modulate gut microbiota, and probiotics have been reported to influence mineral absorption by improving the gut microbiota profile, highlighting the gut-bone axis. This intervention could be relevant in nephrology due to the high prevalence of renal osteodystrophy in chronic kidney disease (CKD). Given the potential role of gut microbiota in bone metabolism, this is the first study to evaluate the effects of consuming Minas Frescal cheese enriched with\n                      <jats:italic>L.\u00a0acidophilus<\/jats:italic>\n                      La-05 on bone parameters in nephrectomized Wistar\u00a0rats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2025-0103_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Rats were divided into Sham and CKD groups receiving conventional or probiotic-enriched Minas cheese (20\u202fg\/day) for 6\u00a0weeks. Bone mineral density (BMD)\u00a0of the femur and tibia was measured using dual-energy X-ray absorptiometry, and femoral biomechanical properties (maximum force, breaking strength, flexural modulus) were assessed using a three-point bending\u00a0test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2025-0103_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CKD significantly reduced tibial (0.08\u00a0\u00b1\u00a00.01 vs. 0.11\u00a0\u00b1\u00a00.01\u202fg\/cm\n                      <jats:sup>3<\/jats:sup>\n                      , p=0.0147) and femoral (0.14\u00a0\u00b1\u00a00.01 vs. 0.16\u00a0\u00b1\u00a00.01\u202fg\/cm\n                      <jats:sup>3<\/jats:sup>\n                      , p=0.0217) BMD in rats, and probiotic supplementation did not mitigate this loss. Probiotic intervention was associated with a significant decrease in femur length in CKD rats (3.74\u00a0\u00b1\u00a00.14 vs. 3.92\u00a0\u00b1\u00a00.10\u202fmm, p=0.0221).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2025-0103_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Short-term probiotic supplementation in a cheese matrix did not improve BMD or femoral biomechanics, suggesting a limited effect on CKD-related bone\u00a0loss.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/jcim-2025-0103","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T07:58:16Z","timestamp":1755849496000},"page":"669-680","source":"Crossref","is-referenced-by-count":1,"title":["Effects of minas frescal cheese enriched with\n                    <i>Lactobacillus acidophilus<\/i>\n                    La-05 on bone health in a preclinical model of chronic kidney disease"],"prefix":"10.1515","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2186-7546","authenticated-orcid":false,"given":"Manuela Fernandes da Silva","family":"Melo","sequence":"first","affiliation":[{"name":"Postgraduate Program in Cardiovascular Sciences , Fluminense Federal University (UFF) , Rua Desembargador Athayde Parreira, 100, Niter\u00f3i, RJ, 24070-090, Niter\u00f3i , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica da Costa","family":"Mota","sequence":"additional","affiliation":[{"name":"College of Nutrition , Fluminense Federal University (UFF) , Rua M\u00e1rio Santos Braga 30, Niter\u00f3i, RJ, 24020-140 Niter\u00f3i , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2380-2295","authenticated-orcid":false,"given":"Joana Ramos de","family":"Araujo","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pathology , Fluminense Federal University (UFF) , Rua Desembargador Athayde Parreira , 100 , 24070-090 , Niter\u00f3i , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9954-6692","authenticated-orcid":false,"given":"Patricia Pereira","family":"Almeida","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pathology , Fluminense Federal University (UFF) , Rua Desembargador Athayde Parreira , 100 , 24070-090 , Niter\u00f3i , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3169-9315","authenticated-orcid":false,"given":"Beatriz Oliveira","family":"Da Cruz","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Cardiovascular Sciences , Fluminense Federal University (UFF) , Rua Desembargador Athayde Parreira, 100, Niter\u00f3i, RJ, 24070-090, Niter\u00f3i , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0898-2083","authenticated-orcid":false,"given":"Michele Lima","family":"Brito","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pathology , Fluminense Federal University (UFF) , Rua Desembargador Athayde Parreira , 100 , 24070-090 , Niter\u00f3i , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato de Souza","family":"Abboud","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pathology , Fluminense Federal University (UFF) , Rua Desembargador Athayde Parreira , 100 , 24070-090 , Niter\u00f3i , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Moreira da","family":"Silva","sequence":"additional","affiliation":[{"name":"Morphology Department, Laboratory of Cellular and Extracellular Biomorphology Biomedical Institute , Fluminense Federal University (UFF) , Niter\u00f3i , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon Silva","family":"Rocha","sequence":"additional","affiliation":[{"name":"Analytical Laboratory of Restorative Biomaterials , 28110 Dental School, Universidade Federal Fluminense (UFF) , Niter\u00f3i , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9285-9669","authenticated-orcid":false,"given":"Adriano Gomes","family":"da Cruz","sequence":"additional","affiliation":[{"name":"Food Department, Federal Institute of Education , Science and Technology of Rio de Janeiro (IFRJ) , Rio de Janeiro , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas de Toledo","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Food Technology Department , Veterinary\u00a0College, Fluminense Federal University (UFF) , Niter\u00f3i , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2660-1800","authenticated-orcid":false,"given":"Nathalia da","family":"Silva-Costa","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Cardiovascular Sciences , Fluminense Federal University (UFF) , Rua Desembargador Athayde Parreira, 100, Niter\u00f3i, RJ, 24070-090, Niter\u00f3i , RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0131-2033","authenticated-orcid":false,"given":"Milena Barcza","family":"Stockler-Pinto","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Cardiovascular Sciences , Fluminense Federal University (UFF) , Rua Desembargador Athayde Parreira, 100, Niter\u00f3i, RJ, 24070-090, Niter\u00f3i , RJ , Brazil"},{"name":"College of Nutrition , Fluminense Federal University (UFF) , Rua M\u00e1rio Santos Braga 30, Niter\u00f3i, RJ, 24020-140 Niter\u00f3i , RJ , Brazil"},{"name":"Postgraduate Program in Pathology , Fluminense Federal University (UFF) , Rua Desembargador Athayde Parreira , 100 , 24070-090 , Niter\u00f3i , RJ , Brazil"},{"name":"Postgraduate Program in Nutrition Sciences , Fluminense Federal University (UFF) , Rua M\u00e1rio Santos Braga 30 , Niter\u00f3i , RJ , 24020-140 , Brazil"},{"name":"Experimental Nutrition Laboratory , Nutrition and Dietetics Department , Fluminense Federal University , Rua M\u00e1rio Santos Braga 30 , Niter\u00f3i , RJ , 24020-140 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,8,22]]},"reference":[{"key":"2026062812010557321_j_jcim-2025-0103_ref_001","doi-asserted-by":"crossref","unstructured":"Connelly, K, Collister, D, Tangri, N. 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