{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T09:41:18Z","timestamp":1767087678281,"version":"3.48.0"},"reference-count":47,"publisher":"Walter de Gruyter GmbH","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_jcim-2025-0132_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Dengue virus (DENV), a single-stranded RNA virus from the Flaviviridae family, causes viral hemorrhagic fever and poses a global health threat due to limited diagnostics, treatments, and vaccines. The conserved NS5 protein, crucial for DENV replication, is a promising antiviral target. Antibody-dependent enhancement (ADE) complicates immunity, as serotype-specific antibodies may worsen infection.\n                      <jats:italic>Moringa oleifera<\/jats:italic>\n                      , rich in antiviral phytochemicals, shows potential as a DENV inhibitor by targeting proteomic, transcriptomic, and metabolomic pathways.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2025-0132_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study employed molecular docking with 3D PubChem structures of bioactive compounds of\n                      <jats:italic>M<\/jats:italic>\n                      .\n                      <jats:italic>oleifera<\/jats:italic>\n                      to evaluate the binding affinity with DENV-2 NS5 proteins (PDB ID: 6KR2) using AutoDock Vina, and binding modes were analyzed using Discovery Studio. Further drug-likeness, oral bioavailability, ADME, and toxicity profiles were analyzed using SwissADME, ADMETSaR, and ADMETlab 3.0 web server. Complexes were subjected to molecular dynamics simulation (MDS) analysis using Desmond Schrodinger v2019.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2025-0132_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Among the screened compounds, rhamnopyranosyl vincosamide (\u22129.0\u202fkcal\/mol), luteoxanthin (\u22128.6\u202fkcal\/mol), and luteolin (\u22128.3\u202fkcal\/mol) exhibited the most stable interactions and were further analyzed through molecular dynamics (MD) simulations. The results revealed that these phytochemicals interact with NS5 active-site residues, demonstrating significant inhibitory potential.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2025-0132_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The study suggests that\n                      <jats:italic>M<\/jats:italic>\n                      .\n                      <jats:italic>oleifera<\/jats:italic>\n                      phytochemicals hold promise as DENV-2 NS5 inhibitors with minimal toxicity and favorable pharmacokinetic properties. These findings provide a strong foundation for further clinical investigations, potentially contributing to developing novel anti-dengue therapeutics.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/jcim-2025-0132","type":"journal-article","created":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T04:56:32Z","timestamp":1761368192000},"page":"644-660","source":"Crossref","is-referenced-by-count":0,"title":["Molecular docking, molecular dynamic simulation, and ADME analysis of\n                    <i>Moringa oleifera<\/i>\n                    phytochemicals targeting NS5 protein: towards the development of novel anti-dengue therapeutics"],"prefix":"10.1515","volume":"22","author":[{"given":"Ashish Kumar","family":"Yadav","sequence":"first","affiliation":[{"name":"Department of Biochemistry , All India Institute of Medical Sciences (AIIMS) , Bhopal , Madhya Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neha","family":"Masarkar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry , All India Institute of Medical Sciences (AIIMS) , Bhopal , Madhya Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maynak","family":"Pal","sequence":"additional","affiliation":[{"name":"Indian Institute of Science Education and Research (IISER) , Bhopal , Madhya Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sukhes","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry , All India Institute of Medical Sciences (AIIMS) , Bhopal , Madhya Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashok","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry , All India Institute of Medical Sciences (AIIMS) , Bhopal , Madhya Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarika","family":"Verma","sequence":"additional","affiliation":[{"name":"Advanced Materials and Processes Research Institute (AMPRI) , Bhopal , Madhya Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rashmi","family":"Chowdhary","sequence":"additional","affiliation":[{"name":"Department of Biochemistry , All India Institute of Medical Sciences (AIIMS) , Bhopal , Madhya Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2025,10,27]]},"reference":[{"key":"2025123009332462494_j_jcim-2025-0132_ref_001","doi-asserted-by":"crossref","unstructured":"Guzman, MG, Alvarez, M, Halstead, SB. 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