{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:29:45Z","timestamp":1789468185786,"version":"build-2803163510"},"reference-count":33,"publisher":"Walter de Gruyter GmbH","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_jcim-2026-0086_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      This study investigated the effects of\n                      <jats:italic>Cannabis sativa<\/jats:italic>\n                      (CS) on hepatotoxicity as well as the protective effects of Vit C against cannabis-induced hepatotoxicity in\u00a0rats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2026-0086_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Different doses (2, 4, and 6\u202fmg) of CS and Vit C (4\u202fmg) were administered orally, both individually and in combination, for a period of three weeks. All animals had unrestricted access to feed and water and were sacrificed at the end of the treatment period. Liver function biomarkers were evaluated using standard analytical methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2026-0086_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Administration of CS at doses of 2, 4, and 6\u202fmg\/kg significantly (p&lt;0.05) increased the levels of gamma-glutamyl transferase (GGT), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin, and creatinine, while significantly (p&lt;0.05) decreasing albumin and induced protein (IP), compared to the control group. The effects were more pronounced at the 6\u202fmg\/kg dose relative to the lower doses. However, co-administration with Vit C significantly attenuated these alterations at the lower doses (2 and 4\u202fmg\/kg) of\u00a0CS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jcim-2026-0086_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings suggest that CS induces alterations in liver function biomarkers indicative of hepatotoxicity, while Vit C exhibits a protective effect at lower exposure levels. Further studies are warranted to determine whether higher doses of Vit C can effectively mitigate liver damage associated with higher doses of\u00a0CS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/jcim-2026-0086","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T08:55:49Z","timestamp":1789462549000},"source":"Crossref","is-referenced-by-count":0,"title":["Mitigating\n                    <i>Cannabis sativa<\/i>\n                    -induced liver toxicity in male Wistar rats: protective role of vitamin C"],"prefix":"10.1515","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5950-0179","authenticated-orcid":false,"given":"Amuda","family":"Oluwasola","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Basic Medical Sciences , University of Ilesa , Ilesa , Osun State , Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"2026091508554195815_j_jcim-2026-0086_ref_001","doi-asserted-by":"crossref","unstructured":"Arif, M, Saifi, MS, Kaish, M, Kushwaha, SP. Cannabis sativa L.-An important medicinal plant: a review of its phytochemistry, pharmacological activities and applications in sustainable economy. IJPPR 2023;14:43\u201359. https:\/\/doi.org\/10.48165\/ijppronline.2023.14305.","DOI":"10.48165\/ijppronline.2023.14305"},{"key":"2026091508554195815_j_jcim-2026-0086_ref_002","unstructured":"Oluwasola, A, Balogun, ME, Muzzammill, SS, Zainab, SO, Adekunle, OJ, Mariam, EB, et al.. Effect of acute administration of ethanol extract of Cannabis sativa leaf on liver function biomarkers in male Wistar rats. NISEB J 2022a;22:20\u20136."},{"key":"2026091508554195815_j_jcim-2026-0086_ref_003","doi-asserted-by":"crossref","unstructured":"Oluwasola, A, Ayoola, OE, Garba, S, Adepoju, MA, Biliaminu, SA, Olayaki, LA. Melatonin mitigates hormonal toxicity in cannabis -treated female Wistar rats. 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Hepatotoxicity and the role of some herbal hepatoprotective plants in present scenario. GJ Dig Dis 2018;3:2.","DOI":"10.4172\/2472-1891.100034"},{"key":"2026091508554195815_j_jcim-2026-0086_ref_026","doi-asserted-by":"crossref","unstructured":"Stauder, R, Valent, P, Theurl, I. Anemia at older age: etiologies, clinical implications, and management. Blood, J Am Soc Hematol 2018;131:505\u201314. https:\/\/doi.org\/10.1182\/blood-2017-07-746446.","DOI":"10.1182\/blood-2017-07-746446"},{"key":"2026091508554195815_j_jcim-2026-0086_ref_027","doi-asserted-by":"crossref","unstructured":"Ndrepepa, G, Kastrati, A. Gamma-glutamyl transferase and cardiovascular disease. Ann Transl Med 2016;4. https:\/\/doi.org\/10.21037\/atm.2016.12.27.","DOI":"10.21037\/atm.2016.12.27"},{"key":"2026091508554195815_j_jcim-2026-0086_ref_028","doi-asserted-by":"crossref","unstructured":"Ozougwu, JC, Eyo, JE. Hepatoprotective effect of Allium cepa (onion) extracts against paracetamol- induced liver damage in rats. Afr J Biotechnol 2014;13:2679\u201388. https:\/\/doi.org\/10.5897\/ajb2014.13815.","DOI":"10.5897\/AJB2014.13815"},{"key":"2026091508554195815_j_jcim-2026-0086_ref_029","doi-asserted-by":"crossref","unstructured":"Cirulli, E, Abramson, K, Andrade, RJ, Bjornsson, ES, Chalasani, N, Nicoletti, P, et al.. Drug-Induced Liver Injury Network (DILIN) investigators; International DILI consortium (iDILIC). A missense variant in PTPN22 is a risk factor for drug-induced liver injury. Gastroenterology 2019;156:1707\u201316. https:\/\/doi.org\/10.1053\/j.gastro.2019.01.034.","DOI":"10.1053\/j.gastro.2019.01.034"},{"key":"2026091508554195815_j_jcim-2026-0086_ref_030","doi-asserted-by":"crossref","unstructured":"Bai, Z, Bernardi, M, Yoshida, EM, Li, H, Guo, X, M\u00e9ndez-S\u00e1nchez, N, et al.. Albumin infusion may decrease the incidence and severity of overt hepatic encephalopathy in liver cirrhosis. 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