{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T04:09:28Z","timestamp":1767499768567},"reference-count":19,"publisher":"Centre for Evaluation in Education and Science (CEON\/CEES)","issue":"4","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,10,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n<jats:p><jats:bold>Background:<\/jats:bold> Serum small dense LDL-cholesterol (sdLDL-C) value is suggested to be an important risk factor for atherosclerosis. Since sdLDL-C changes may be related to PCSK9 and SREBP-2 functions, the aim of this study was to investigate correlations between sdLDL-C, circulating PCSK9, SREBP-2 expression and some lipid parameters in serum and buffy coat fraction of healthy subjects.<\/jats:p>\n<jats:p><jats:bold>Methods:<\/jats:bold> One hundred and twenty-four subjects were randomly included in the study. The lipid profile was measured using routine laboratory methods. The serum sdLDL-C level was calculated by a heparin-related precipitation technique. The cellular LDL-C\/protein and cholesterol\/protein values were measured after lysing of cells with methanol\/chloroform binary solvent. The circulating PCSK9 level was measured using ELISA technique. The SREBP-2 expression level was estimated using the RT-qPCR technique.<\/jats:p>\n<jats:p><jats:bold>Results:<\/jats:bold> Data showed significant correlations between LDL-C, TG and sdLDL-C levels (r=0.34, p=0.001; r=0.2, p=0.04). The circulating PCSK9 level was correlated to LDL-C (r=0.29, p=0.04), but not to sdLDL-C (r=\u22120.08, p=0.57). Also, cellular LDL-C value was not related to serum LDL-C level (r=\u22120.12, p=0.39). Furthermore, there was an inverse correlation between cellular LDL-C\/protein value and estimated <jats:italic>de novo<\/jats:italic> cholesterol\/protein value (r=\u22120.5, p=0.001). Similar results were observed for cellular LDL-C\/protein value and SREBP-2 expression level (r=\u22120.52, p=0.004).<\/jats:p>\n<jats:p><jats:bold>Conclusions:<\/jats:bold> We concluded that the serum sdLDL-C value is not related to circulating PCSK9. Furthermore, SREBP-2 regulatory system was able to elevate the cellular cholesterol level after reducing LDL influx. We suggest to investigate the cellular sdLDL fate and lipid synthesis pathways in PCSK9-targeting studies.<\/jats:p>","DOI":"10.1515\/jomb-2016-0019","type":"journal-article","created":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T09:51:57Z","timestamp":1474365117000},"page":"410-415","source":"Crossref","is-referenced-by-count":12,"title":["Serum sdLDL-C and Cellular SREBP2-Dependent Cholesterol Levels; Is there a Challenge on Targeting PCSK9?"],"prefix":"10.5937","volume":"35","author":[{"given":"Elham","family":"Softanmohammadi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadegh","family":"Piran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asghar","family":"Mohammadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bita","family":"Hosseni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faezeh","family":"Naseri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Shabani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Najafi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3964","reference":[{"key":"ref141","doi-asserted-by":"crossref","first-page":"160","DOI":"10.2478\/jomb-2014-0037","article-title":"Oxidized low density lipoprotein and high sensitive c - 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dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"ref171","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/qjmed\/hci154","article-title":"Low - density lipoprotein size and cardiovascular risk assessment","volume":"99","author":"Rizzo","year":"2006","journal-title":"QJM"},{"key":"ref31","first-page":"414","article-title":"Small dense LDL : An emerging risk factor for cardiovascular disease","author":"Hirayama","year":"2012","journal-title":"Clin Chim Acta"},{"key":"ref181","first-page":"436","article-title":"How Do PCSK Inhibitors Stack Up to Statins for Low - Density Lipoprotein Cholesterol Control","volume":"9","author":"Zimmerman","year":"2015","journal-title":"Am Health Drug Benefits"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1007\/s11883-999-0050-6","article-title":"Small dense low - density lipoprotein subclass pattern issues for the clinician","volume":"1","author":"Superko","year":"1999","journal-title":"Curr Atheroscler Rep"},{"key":"ref101","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/o59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can J Biochem Physiol"},{"key":"ref61","first-page":"493","article-title":"New developments in atherosclerosis : clinical potential of PCSK inhibition","volume":"9","author":"Giunzioni","year":"2015","journal-title":"Vase Health Risk Manag"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1126\/scisignal.aac5124","article-title":"Neuregulin - 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