{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T15:29:34Z","timestamp":1774020574715,"version":"3.50.1"},"reference-count":20,"publisher":"Centre for Evaluation in Education and Science (CEON\/CEES)","issue":"2","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,4,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background:<\/jats:bold>Infertility is an important worldwide problem which affects 10\u201315% of couples globally. Altered NO production has also been implicated in the pathogenesis of the male infertility. The present study was designed to evaluate the changes in the activity of NO-synthase (NOS) and arginase in spermatozoa of patients with infertility.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold>The total NOS, Ca<jats:sup>2+<\/jats:sup>-dependent constitutive (cNOS) and Ca<jats:sup>2+<\/jats:sup>-independent inducible (iNOS) activity and arginase activity were assessed in sperm cells of patients with different forms of pathospermia.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold>We found a significant increase in iNOS activity, but significantly decreased cNOS and arginase activity in sperm cells of infertile men vs fertile, normozoospermic men (p&lt;0.001). The arginase\/NOS ratio significantly decreased compared to control group. The iNOS\/cNOS ratio was drastically increased in patients with decreased fertility potential indicating predominance of iNOS. Men with leuko cytospermia were distinguished to have the most express iNOS activity.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold>These observations provide evidence for a disturbed balance between the L-arginine metabolic pathways in sperm cells of infertile men. This imbalance includes the considerable activation of the inducible isoform of NO-synthase accompanied by significant inhibition of its constitutive isoform which indicates disturbances in NO production. In patients with decreased fertility potential the arginase\/NOS was shifted towards predominance of iNOS-derived NO production.<\/jats:p>","DOI":"10.1515\/jomb-2017-0049","type":"journal-article","created":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T22:15:55Z","timestamp":1510265755000},"page":"134-140","source":"Crossref","is-referenced-by-count":13,"title":["Alterations in Arginase-No-Synthase System of Spermatozoa in Human Subjects with Different Fertility Potential"],"prefix":"10.5937","volume":"37","author":[{"given":"Roman V.","family":"Fafula","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulyana P.","family":"Iefremova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olena K.","family":"Onufrovych","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanna V.","family":"Maksymyuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna S.","family":"Besedina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iosyf A.","family":"Nakonechnyi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmytro Z.","family":"Vorobets","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zinoviy D.","family":"Vorobets","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3964","reference":[{"key":"ref111","first-page":"37","article-title":"The biphasic nature of nitric oxide responses in tumor Antioxidants","volume":"12","author":"Ridnour","year":"2006","journal-title":"biology Redox Signaling"},{"key":"ref01","first-page":"1","article-title":"unique view on male infertility around the globe","author":"Agarwal","journal-title":"Reprod Biol"},{"key":"ref81","first-page":"193","article-title":"OH Protein measurement with the Folin phenolreagent","author":"Lowry","year":"1951","journal-title":"J Biol Chem"},{"key":"ref221","first-page":"318","article-title":"Mitochondrial arginase II modulates nitric - oxide synthesis through nonfreely exchangeable arginine pools in human endothelial cells","volume":"23","author":"Topal","year":"2006","journal-title":"J Pharmacol Exp Ther"},{"key":"ref231","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1515\/jomb-2017-0032","article-title":"medicine is faced with the evolution of medical practice","volume":"24","author":"Collinson","year":"2017","journal-title":"Laboratory J Med Biochem"},{"key":"ref61","article-title":"Impact of seminal trace element and glutathione levels on semen quality of Tunisian infertile men","volume":"12","author":"Atig","year":"2012"},{"key":"ref101","first-page":"5323","article-title":"Role of oxidative stress and nitric oxide in atherothrombosis","volume":"11","author":"Lubos","year":"2009","journal-title":"Front Biosci"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1161\/01.HYP.0000198543.34502.d7","article-title":"Knockdown of arginase I restores NO signaling in the vasculature of old rats","author":"White","year":"2006","journal-title":"Hypertension"},{"key":"ref241","article-title":"Seminal arginase activity in infertility","volume":"25","author":"Elgun","year":"2000","journal-title":"Urol Res"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1042\/BST20140122","article-title":"The red blood cell : a new key player in cardiovascular homeostasis ? 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