{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T09:39:44Z","timestamp":1775381984810,"version":"3.50.1"},"reference-count":8,"publisher":"Walter de Gruyter GmbH","issue":"7-8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Perforin plays an essential role in cytotoxicity of natural killers (NK) and CD8+ lymphocytes. Cytotoxicity of T and NK cells is one of the mechanisms of destruction of cells in Hashimoto\u2019s disease (HD). The aim of this study was analysis of the expression of perforin in CD8+, CD4+, and NK cells and cytotoxic abilities of these cells in children with HD compared to healthy controls. The expression of perforin and surface antigens, as well as cytotoxicity were analyzed with a flow cytometry. Lower expression of perforin in CD8+ and NK was found in HD compared to controls (p=0.01; p=0.004). A significant correlation between perforin expression in CD8+ lymphocytes and in NK was observed (p=0.05). The spontaneous cytotoxicity of NK was significantly higher in HD compared to controls (p=0.04). Our results suggest that perforin plays an important role in the pathogenesis of autoimmune Hashimoto\u2019s thyroiditis.<\/jats:p>","DOI":"10.1515\/jpem-2014-0520","type":"journal-article","created":{"date-parts":[[2015,7,13]],"date-time":"2015-07-13T08:57:51Z","timestamp":1436777871000},"source":"Crossref","is-referenced-by-count":7,"title":["Cytometric analysis of perforin expression in NK cells, CD8+, and CD4+ lymphocytes in children with autoimmune Hashimoto\u2019s thyroiditis \u2013 a preliminary study"],"prefix":"10.1515","volume":"28","author":[{"given":"Katarzyna","family":"Popko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iwona","family":"Osi\u0144ska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Kucharska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urszula","family":"Demkow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref21","first-page":"470","article-title":"expression by thyroid infiltrating cells in autoimmune thyroid disease","author":"Wu","year":"1994","journal-title":"Clin Exp Immunol"},{"key":"ref81","first-page":"470","article-title":"expression by thyroid infiltrating cells in autoimmune thyroid disease","author":"Wu","year":"1994","journal-title":"Clin Exp Immunol"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1515\/jpem-2013-0373","article-title":"The natural course of Hashimoto s thyroiditis in children and adolescents","volume":"27","author":"Lee","year":"2014","journal-title":"J Pediatr Endocrinol Metab"},{"key":"ref51","first-page":"1037","article-title":"Deb axons and motor function are protected by genetic deletion of perforin","author":"Lafrance","year":"2009","journal-title":"Exp Neurol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1515\/jpem-2013-0373","article-title":"The natural course of Hashimoto s thyroiditis in children and adolescents","volume":"27","author":"Lee","year":"2014","journal-title":"J Pediatr Endocrinol Metab"},{"key":"ref61","first-page":"235","article-title":"structure function and role in human immunopathology","author":"Voskoboinik","year":"2010","journal-title":"Immunol Rev"},{"key":"ref01","first-page":"235","article-title":"structure function and role in human immunopathology","author":"Voskoboinik","year":"2010","journal-title":"Immunol Rev"},{"key":"ref111","first-page":"1037","article-title":"Deb axons and motor function are protected by genetic deletion of perforin","author":"Lafrance","year":"2009","journal-title":"Exp Neurol"}],"container-title":["Journal of Pediatric Endocrinology and Metabolism"],"original-title":[],"link":[{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpem-2014-0520\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T01:38:44Z","timestamp":1619055524000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpem-2014-0520\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,1]]},"references-count":8,"journal-issue":{"issue":"7-8"},"URL":"https:\/\/doi.org\/10.1515\/jpem-2014-0520","relation":{},"ISSN":["2191-0251","0334-018X"],"issn-type":[{"value":"2191-0251","type":"electronic"},{"value":"0334-018X","type":"print"}],"subject":[],"published":{"date-parts":[[2015,1,1]]}}}