{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T09:18:04Z","timestamp":1721899084143},"reference-count":54,"publisher":"Walter de Gruyter GmbH","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,1,26]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>\u03b1-Klotho is a transmembrane protein that can be cleaved and act as a circulating hormone (s-klotho). s-Klotho serum levels seem to reflect growth hormone (GH) secretory status. We investigated the role of s-klotho as a reliable marker of GH secretion in short children and the factors influencing its secretion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>We enrolled 40 short Egyptian children (20 GH deficiency [GHD] and 20 idiopathic short stature [ISS]). They underwent a pegvisomant-primed insulin tolerance test (ITT) and were accordingly reclassified as 16 GHD and 24 ISS. The samples obtained before and 3 days after pegvisomant administration, prior to the ITT, were used for assaying insulin-like growth factor (IGF)-I and s-klotho.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>IGF-I and s-klotho serum levels were not significantly different (p=0.059 and p=0.212, respectively) between GHD and ISS. After pegvisomant, a significant reduction in IGF-I and s-klotho levels was found in both groups. s-Klotho significantly correlated only with IGF-I levels in both groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>s-Klotho mainly reflects the IGF-I status and cannot be considered a reliable biomarker for GH secretion in children.<\/jats:p><\/jats:sec>","DOI":"10.1515\/jpem-2016-0464","type":"journal-article","created":{"date-parts":[[2017,9,13]],"date-time":"2017-09-13T10:01:08Z","timestamp":1505296868000},"source":"Crossref","is-referenced-by-count":3,"title":["Serum \u03b1-klotho levels are not informative for the evaluation of growth hormone secretion in short children"],"prefix":"10.1515","volume":"30","author":[{"given":"Cristina","family":"Meazza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heba H.","family":"Elsedfy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randa I.","family":"Khalaf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fiorenzo","family":"Lupi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Pagani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed El","family":"Kholy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmine","family":"Tinelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giorgio","family":"Radetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Bozzola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref81","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1046\/j.1365-2265.2002.01591.x","article-title":"Height velocity and IGF.I assessment in the diagnosis of childhood onset GH insufficiency: do we still need a second GH stimulation test?","volume":"57","year":"2002","journal-title":"Clin Endocrinol (Oxf)"},{"key":"ref251","first-page":"499","article-title":"Insulin-like growth factor-I is a marker for the nutritional state","volume":"13","year":"2015","journal-title":"Pediatr Endocrinol Rev"},{"key":"ref531","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/0014-5793(93)81672-M","article-title":"Growth hormone, but not insulin-like growth factor I, induces a serum protease activity for insulin-like growth factor binding protein-3 in hypophysectomised rats in vivo","volume":"334","year":"1993","journal-title":"FEBS Lett"},{"key":"ref321","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/BF03345583","article-title":"Unreliability of classic provocative tests for the diagnosis of growth hormone deficiency","volume":"31","year":"2008","journal-title":"J Endocrinol Invest"},{"key":"ref411","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/j.bbagen.2009.02.005","article-title":"Klotho and aging","volume":"1790","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"ref191","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1530\/EJE-15-1018","article-title":"The effect of GH treatment on serum FGF23 and klotho in GH-deficient children","volume":"174","year":"2016","journal-title":"Eur J Endocrinol"},{"key":"ref511","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1159\/000342577","article-title":"Insulin-like growth factors, nutrition and growth","volume":"106","year":"2013","journal-title":"World Rev Nutr Diet"},{"key":"ref331","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1046\/j.1365-2265.2003.01773.x","article-title":"Growth hormone secretory pattern and response to treatment in children with short stature followed to adult height","volume":"59","year":"2003","journal-title":"Clin Endocrinol (Oxf)"},{"key":"ref441","first-page":"E326","article-title":"The aging suppressor klotho: a potential regulator of growth hormone secretion. 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Study in 472 normally growing children","volume":"8","year":"1996","journal-title":"J Clin Endocrinol Metab"},{"key":"ref361","article-title":"Is retesting in growth hormone deficient children really useful?","year":"2016","journal-title":"Minerva Endocrinol"},{"key":"ref341","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1111\/j.1365-2265.2007.03132.x","article-title":"Pegvisomant-primed GH stimulation test","volume":"68","year":"2008","journal-title":"Clin Endocrinol (Oxf)"},{"key":"ref181","doi-asserted-by":"crossref","first-page":"e107174","DOI":"10.1371\/journal.pone.0107174","article-title":"Association between decreased klotho blood levels and organic growth hormone deficiency in children with growth impairment","volume":"9","year":"2014","journal-title":"PLoS One"},{"key":"ref231","first-page":"654","article-title":"Reference ranges for two automated chemiluminescent assays for serum insulin-like growth factor I (IGF-I) and IGF-binding protein 3 (IGFBP-3)","volume":"42","year":"2004","journal-title":"Clin Chem Lab Med"},{"key":"ref01","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1210\/jcem-71-1-230","article-title":"Variability of growth hormone response to pharmacological and sleep tests performed twice in short children","volume":"71","year":"1990","journal-title":"J Clin Endocrinol Metab"},{"key":"ref221","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1002\/(SICI)1097-0258(19980228)17:4<407::AID-SIM742>3.0.CO;2-L","article-title":"British 1990 growth reference centiles for weight, height, body mass index and head circumference fitted by maximum penalized likelihood","volume":"17","year":"1998","journal-title":"Stat Med"},{"key":"ref481","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1136\/adc.41.219.454","article-title":"Standards from birth to maturity for height, weight, height velocity and weight velocity: british children, 1965. I","volume":"41","year":"1966","journal-title":"Arch Dis Child"},{"key":"ref521","first-page":"499","article-title":"Insulin-like growth factor-I is a marker for the nutritional state","volume":"13","year":"2015","journal-title":"Pediatr Endocrinol Rev"},{"key":"ref91","article-title":"Is retesting in growth hormone deficient children really useful?","year":"2016","journal-title":"Minerva Endocrinol"},{"key":"ref121","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.febslet.2004.03.090","article-title":"Secreted klotho protein in sera and CSF: implication for post-translational cleavage in release of klotho protein from cell membrane","volume":"565","year":"2004","journal-title":"FEBS Lett"},{"key":"ref211","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1136\/adc.41.219.454","article-title":"Standards from birth to maturity for height, weight, height velocity and weight velocity: british children, 1965. I","volume":"41","year":"1966","journal-title":"Arch Dis Child"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1111\/j.1365-2265.2007.03132.x","article-title":"Pegvisomant-primed GH stimulation test","volume":"68","year":"2008","journal-title":"Clin Endocrinol (Oxf)"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/BF03345583","article-title":"Unreliability of classic provocative tests for the diagnosis of growth hormone deficiency","volume":"31","year":"2008","journal-title":"J Endocrinol Invest"}],"container-title":["Journal of Pediatric Endocrinology and Metabolism"],"original-title":[],"link":[{"URL":"http:\/\/www.degruyter.com\/view\/j\/jpem.2017.30.issue-10\/jpem-2016-0464\/jpem-2016-0464.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpem-2016-0464\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T01:42:01Z","timestamp":1619055721000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpem-2016-0464\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,1]]},"references-count":54,"journal-issue":{"issue":"10"},"URL":"https:\/\/doi.org\/10.1515\/jpem-2016-0464","relation":{},"ISSN":["2191-0251","0334-018X"],"issn-type":[{"value":"2191-0251","type":"electronic"},{"value":"0334-018X","type":"print"}],"subject":[],"published":{"date-parts":[[2017,1,1]]}}}