{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:52:48Z","timestamp":1791337968985,"version":"4.3.1"},"reference-count":20,"publisher":"Walter de Gruyter GmbH","issue":"5","funder":[{"name":"ESPE","award":["Early Career Scientific Development Grant der Euro"],"award-info":[{"award-number":["Early Career Scientific Development Grant der Euro"]}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["314061271-TRR\/CRC 205-1\/2 to Martin Bidlingmaier"],"award-info":[{"award-number":["314061271-TRR\/CRC 205-1\/2 to Martin Bidlingmaier"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_jpem-2024-0027_abs_001\">\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The most suitable biochemical markers for therapy adjustment in patients with congenital adrenal hyperplasia are controversial. 11-Oxygenated androgens are a promising new approach. The objective of this study was to investigate the diurnal rhythm of 11-ketotestosterone in children and adolescents in saliva and to correlate it with salivary 17-hydroxyprogesterone.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpem-2024-0027_abs_002\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Fifty-one samples of steroid day-profiles from 17 patients were additionally analysed for 11-ketotestosterone, retrospectively. All patients were treated in our university outpatient clinic for paediatric endocrinology between 2020 and 2022. Steroid day-profiles of 17 patients could be examined. The cohort showed a balanced sex ratio. The median age was 13 years. The measurements for 17-hydroxyprogesterone were carried out during routine care by immunoassay. The measurements of 11-ketotestosterone were performed from frozen saliva samples using an implemented in-house protocol for liquid chromatography\u2013tandem mass spectrometry (LC-MS\/MS). The most important outcome were the absolute values for 11-ketotestosterone, their diurnal rhythmicity and the correlation with 17-hydroxyprogesterone.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpem-2024-0027_abs_003\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Both steroids show a circadian diurnal rhythm. 17-hydroxyprogesterone and 11-ketotestosterone correlate significantly. 11-Ketotestosterone showed a positive correlation with BMI at all times of the day.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpem-2024-0027_abs_004\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>11-Ketotestosterone shows circadian rhythmicity in our cohort and correlates with 17-hydroxyprogesterone. These findings serve as an important basis for prospective research into 11-oxygenated androgens as therapeutic markers in paediatrics. However, 11-ketotestosterone appears to be very dependent on BMI.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/jpem-2024-0027","type":"journal-article","created":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T16:10:35Z","timestamp":1711987835000},"page":"419-424","source":"Crossref","is-referenced-by-count":2,"title":["Diurnal 11-ketotestosterone and 17-hydroxyprogesterone saliva profiles in paediatric classical congenital adrenal hyperplasia"],"prefix":"10.1515","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3670-2453","authenticated-orcid":false,"given":"Ilja","family":"Dubinski","sequence":"first","affiliation":[{"name":"Department of Paediatrics, Division of Paediatric Endocrinology , 74939 Dr. von Hauner Children\u2019s Hospital, University Hospital, LMU Munich , Munich , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Bechtold-Dalla Pozza","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Division of Paediatric Endocrinology , 74939 Dr. von Hauner Children\u2019s Hospital, University Hospital, LMU Munich , Munich , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Bidlingmaier","sequence":"additional","affiliation":[{"name":"Department of Medicine IV , 74939 University Hospital, LMU Munich , Munich , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Hawley","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry , 5293 Manchester University Foundation NHS Trust, Manchester Academic Health Sciences Centre , Manchester , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Keevil","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry , 5293 Manchester University Foundation NHS Trust, Manchester Academic Health Sciences Centre , Manchester , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Kunz","sequence":"additional","affiliation":[{"name":"Department of Medicine IV , 74939 University Hospital, LMU Munich , Munich , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah Franziska","family":"Nowotny","sequence":"additional","affiliation":[{"name":"Department of Medicine IV , 74939 University Hospital, LMU Munich , Munich , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Reisch","sequence":"additional","affiliation":[{"name":"Department of Medicine IV , 74939 University Hospital, LMU Munich , Munich , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Schiergens","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Division of Paediatric Endocrinology , 74939 Dr. von Hauner Children\u2019s Hospital, University Hospital, LMU Munich , Munich , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Tschaidse","sequence":"additional","affiliation":[{"name":"Department of Medicine IV , 74939 University Hospital, LMU Munich , Munich , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinrich","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Division of Paediatric Endocrinology , 74939 Dr. von Hauner Children\u2019s Hospital, University Hospital, LMU Munich , Munich , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2024,4,2]]},"reference":[{"key":"2024081908520418841_j_jpem-2024-0027_ref_001","doi-asserted-by":"crossref","unstructured":"El-Maouche, D, Arlt, W, Merke, DP. Congenital adrenal hyperplasia. Lancet 2017;390:2194\u2013210. Erratum in: Lancet. 2017;390:2142. https:\/\/doi.org\/10.1016\/S0140-6736(17)31431-9.","DOI":"10.1016\/S0140-6736(17)31431-9"},{"key":"2024081908520418841_j_jpem-2024-0027_ref_002","doi-asserted-by":"crossref","unstructured":"Mallappa, A, Merke, DP. Management challenges and therapeutic advances in congenital adrenal hyperplasia. Nat Rev Endocrinol 2022;18:337\u201352. https:\/\/doi.org\/10.1038\/s41574-022-00655-w.","DOI":"10.1038\/s41574-022-00655-w"},{"key":"2024081908520418841_j_jpem-2024-0027_ref_003","doi-asserted-by":"crossref","unstructured":"Claahsen-van der Grinten, HL, Speiser, PW, Ahmed, SF, Arlt, W, Auchus, RJ, Falhammar, H, et al.. Congenital adrenal hyperplasia-current insights in pathophysiology, diagnostics, and management. 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Erratum in: J Clin Endocrinol Metab. 2019;104:39\u201340. https:\/\/doi.org\/10.1210\/jc.2018-01865.","DOI":"10.1210\/jc.2018-01865"},{"key":"2024081908520418841_j_jpem-2024-0027_ref_006","doi-asserted-by":"crossref","unstructured":"Turcu, AF, Mallappa, A, Elman, MS, Avila, NA, Marko, J, Rao, H, et al.. 11-Oxygenated androgens are biomarkers of adrenal volume and testicular adrenal rest tumors in 21-hydroxylase deficiency. J Clin Endocrinol Metab 2017;102:2701\u201310. https:\/\/doi.org\/10.1210\/jc.2016-3989.","DOI":"10.1210\/jc.2016-3989"},{"key":"2024081908520418841_j_jpem-2024-0027_ref_007","doi-asserted-by":"crossref","unstructured":"Turcu, AF, Nanba, AT, Chomic, R, Upadhyay, SK, Giordano, TJ, Shields, JJ, et al.. Adrenal-derived 11-oxygenated 19-carbon steroids are the dominant androgens in classic 21-hydroxylase deficiency. 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Horm Res Paediatr 2018;89:284\u201391. https:\/\/doi.org\/10.1159\/000486036.","DOI":"10.1159\/000486036"},{"key":"2024081908520418841_j_jpem-2024-0027_ref_012","doi-asserted-by":"crossref","unstructured":"Bacila, I, Adaway, J, Hawley, J, Mahdi, S, Krone, R, Patel, L, et al.. Measurement of salivary adrenal-specific androgens as biomarkers of therapy control in 21-hydroxylase deficiency. J Clin Endocrinol Metab 2019;104:6417\u201329. https:\/\/doi.org\/10.1210\/jc.2019-00031.","DOI":"10.1210\/jc.2019-00031"},{"key":"2024081908520418841_j_jpem-2024-0027_ref_013","doi-asserted-by":"crossref","unstructured":"Breslow, E, Taylor, A, Chan, CL, Severn, C, Pyle, L, Torchen, L, et al.. 11-Oxygenated androgen metabolite concentrations are affected by pubertal progression and obesity. 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Eur J Endocrinol 2023;188:lvac017. https:\/\/doi.org\/10.1093\/ejendo\/lvac017.","DOI":"10.1093\/ejendo\/lvac017"}],"container-title":["Journal of Pediatric Endocrinology and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpem-2024-0027\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpem-2024-0027\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T08:52:57Z","timestamp":1724057577000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpem-2024-0027\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,2]]},"references-count":20,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,3,7]]},"published-print":{"date-parts":[[2024,5,27]]}},"alternative-id":["10.1515\/jpem-2024-0027"],"URL":"https:\/\/doi.org\/10.1515\/jpem-2024-0027","relation":{},"ISSN":["0334-018X","2191-0251"],"issn-type":[{"value":"0334-018X","type":"print"},{"value":"2191-0251","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,2]]}}}