{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:46:53Z","timestamp":1763038013054,"version":"3.40.5"},"reference-count":30,"publisher":"Walter de Gruyter GmbH","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_jpem-2024-0283_abs_001\">\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Gonadotropin-releasing hormone (GnRH) test is the gold standard test to evaluate the hypothalamus\u2013pituitary\u2013gonadal (HPG) axis for the diagnosis of central precocious puberty (CPP). However, the diagnosis of cases with clinical features of CPP whilst have borderline peak luteinizing hormone (LH) remain challenges. We aimed to evaluate diagnostic performance of the average of LH levels measured during GnRH stimulation test.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpem-2024-0283_abs_002\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Cases with diagnosis of CPP and premature thelarche (PT) who had a GnRH stimulation test results were retrospectively reviewed. Anthropometric measurements (weight, height, and body mass index), age and sex-specific standard deviation scores, growth velocity, puberty stages, bone ages, serum FSH, LH, and estradiol levels were measured by electrochemiluminescence immunological method (ECLIA), and the GnRH stimulation test results, which performed by obtaining venous blood samples at basal, 20<jats:sup>th<\/jats:sup>, and 40<jats:sup>th<\/jats:sup> minutes for FSH and LH measurement, were recorded.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpem-2024-0283_abs_003\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>A total of 76 girls (38 CPP, 38 PT) were included. We detected an average peak LH cut-off value of 4.25\u202fIU\/L with 94.7\u202f% sensitivity and 97.4\u202f% specificity, a 97.3\u202f% positive predictive value, and a 94.9\u202f% negative predictive value in GnRH test to differentiate cases with CPP from PT.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpem-2024-0283_abs_004\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This is the first study evaluating the diagnostic utility of the average of LH levels measured during GnRH stimulation test. We showed that the average of two LH measurements has a high diagnostic performance. Therefore, it can be used as a valid and reliable diagnostic tool for assessment of HPG axis activation, particularly for cases with a borderline peak LH level.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/jpem-2024-0283","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T20:01:10Z","timestamp":1723838470000},"page":"773-778","source":"Crossref","is-referenced-by-count":6,"title":["Diagnostic utility of the average peak LH levels measured during GnRH stimulation test"],"prefix":"10.1515","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9724-2369","authenticated-orcid":false,"given":"Serkan Bilge","family":"Koca","sequence":"first","affiliation":[{"name":"Kayseri City Education and Research Hospital , Department of Pediatrics , Division of Pediatric Endocrinology , Kayseri , T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00fcseyin","family":"Demirbilek","sequence":"additional","affiliation":[{"name":"Hacettepe University , Faculty of Medicine , Department of Pediatrics , Division of Pediatric Endocrinology , Ankara , T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2024,8,19]]},"reference":[{"key":"2024090408434236883_j_jpem-2024-0283_ref_001","doi-asserted-by":"crossref","unstructured":"Parent, AS, Teilmann, G, Juul, A, Skakkebaek, NE, Toppari, J, Bourguignon, JP. The timing of normal puberty and the age limits of sexual precocity: variations around the world, secular trends, and changes after migration. Endocr Rev 2003;24:668\u201393. https:\/\/doi.org\/10.1210\/er.2002-0019.","DOI":"10.1210\/er.2002-0019"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_002","doi-asserted-by":"crossref","unstructured":"Carel, JC, L\u00e9ger, J. Clinical practice. Precocious puberty. N Engl J Med 2008;358:2366\u201377. https:\/\/doi.org\/10.1056\/NEJMcp0800459.","DOI":"10.1056\/NEJMcp0800459"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_003","doi-asserted-by":"crossref","unstructured":"Kletter, GB, Klein, KO, Wong, YY. A pediatrician\u2019s guide to central precocious puberty. Clin Pediatr 2015;54:414\u201324. https:\/\/doi.org\/10.1177\/0009922814541807.","DOI":"10.1177\/0009922814541807"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_004","doi-asserted-by":"crossref","unstructured":"Pasquino, AM, Tebaldi, L, Cioschi, L, Cives, C, Finocchi, G, Maciocci, M, et al.. Premature thelarche: a follow up study of 40 girls. Natural history and endocrine findings. Arch Dis Child 1985;60:1180\u20132. https:\/\/doi.org\/10.1136\/adc.60.12.1180.","DOI":"10.1136\/adc.60.12.1180"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_005","doi-asserted-by":"crossref","unstructured":"Neely, EK, Wilson, DM, Lee, PA, Stene, M, Hintz, RL. Spontaneous serum gonadotropin concentrations in the evaluation of precocious puberty. J Pediatr 1995;127:47\u201352. https:\/\/doi.org\/10.1016\/s0022-3476(95)70255-5.","DOI":"10.1016\/S0022-3476(95)70255-5"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_006","doi-asserted-by":"crossref","unstructured":"Bhatia, S, Neely, EK, Wilson, DM. Serum luteinizing hormone rises within minutes after depot leuprolide injection: implications for monitoring therapy. Pediatrics 2002;109:E30. https:\/\/doi.org\/10.1542\/peds.109.2.e30.","DOI":"10.1542\/peds.109.2.e30"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_007","doi-asserted-by":"crossref","unstructured":"Resende, EA, Lara, BH, Reis, JD, Ferreira, BP, Pereira, GA, Borges, MF. Assessment of basal and gonadotropin-releasing hormone-stimulated gonadotropins by immunochemiluminometric and immunofluorometric assays in normal children. J Clin Endocrinol Metab 2007;92:1424\u20139. https:\/\/doi.org\/10.1210\/jc.2006-1569.","DOI":"10.1210\/jc.2006-1569"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_008","doi-asserted-by":"crossref","unstructured":"\u00c7atl\u0131, G, Erdem, P, An\u0131k, A, Abac\u0131, A, B\u00f6ber, E. Clinical and laboratory findings in the differential diagnosis of central precocious puberty and premature thelarche. Turk Pediatr Ars 2015;50:20\u20136. https:\/\/doi.org\/10.5152\/tpa.2015.2281.","DOI":"10.5152\/tpa.2015.2281"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_009","doi-asserted-by":"crossref","unstructured":"Vuralli, D, Ciftci, N, Demirbilek, H. Serum kisspeptin, neurokinin B and inhibin B levels can be used as alternative parameters to distinguish idiopathic CPP from premature thelarche in the early stages of puberty. Clin Endocrinol 2023;98:788\u201395. https:\/\/doi.org\/10.1111\/cen.14906.","DOI":"10.1111\/cen.14906"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_010","doi-asserted-by":"crossref","unstructured":"Be\u015fta\u015f, A, Unal, E, Aktar Karakaya, A, Demiral, M, Haspolat, YK. Evaluation of clinical and laboratory findings in the differential diagnosis of central precocious puberty and premature thelarche. 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Comput Math Methods Med 2022;2022:4118911. https:\/\/doi.org\/10.1155\/2022\/4118911.","DOI":"10.1155\/2022\/4118911"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_017","doi-asserted-by":"crossref","unstructured":"Fu, JF, Liang, JF, Zhou, XL, Prasad, HC, Jin, JH, Dong, GP, et al.. Impact of BMI on gonadorelin-stimulated LH peak in premenarcheal girls with idiopathic central precocious puberty. Obesity 2015;23:637\u201343. https:\/\/doi.org\/10.1002\/oby.21010.","DOI":"10.1002\/oby.21010"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_018","doi-asserted-by":"crossref","unstructured":"Demir, K, \u00d6zen, S, Konak\u00e7\u0131, E, Ayd\u0131n, M, Darendeliler, F. A comprehensive online calculator for pediatric endocrinologists: \u00c7EDD \u00c7\u00f6z\u00fcm\/TPEDS metrics. 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Endocr Connect 2021;10:164\u201370. https:\/\/doi.org\/10.1530\/EC-20-0651.","DOI":"10.1530\/EC-20-0651"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_024","doi-asserted-by":"crossref","unstructured":"Cao, R, Liu, J, Fu, P, Zhou, Y, Li, Z, Liu, P. The diagnostic utility of the basal luteinizing hormone level and single 60-minute post GnRH agonist stimulation test for idiopathic central precocious puberty in girls. Front Endocrinol 2021;12:713880. https:\/\/doi.org\/10.3389\/fendo.2021.713880.","DOI":"10.3389\/fendo.2021.713880"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_025","doi-asserted-by":"crossref","unstructured":"Cavallo, A, Richards, GE, Busey, S, Michaels, SE. A simplified gonadotrophin-releasing hormone test for precocious puberty. 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Korean J Lab Med 2011;31:244\u20139. https:\/\/doi.org\/10.3343\/kjlm.2011.31.4.244.","DOI":"10.3343\/kjlm.2011.31.4.244"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_028","doi-asserted-by":"crossref","unstructured":"Kandemir, N, Demirbilek, H, \u00d6z\u00f6n, ZA, G\u00f6n\u00e7, N, Alika\u015fifo\u011flu, A. GnRH stimulation test in precocious puberty: single sample is adequate for diagnosis and dose adjustment. J Clin Res Pediatr Endocrinol 2011;3:12\u20137. https:\/\/doi.org\/10.4274\/jcrpe.v3i1.03.","DOI":"10.4274\/jcrpe.v3i1.03"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_029","doi-asserted-by":"crossref","unstructured":"Chotipakornkul, N, Onsoi, W, Numsriskulrat, N, Aroonparkmongkol, S, Supornsilchai, V, Srilanchakon, K. The utilization of basal luteinizing hormone in combination with the basal luteinizing hormone and follicle-stimulating hormone ratio as a diagnostic tool for central precocious puberty in girls. Ann Pediatr Endocrinol Metab 2023;28:138\u201343. https:\/\/doi.org\/10.6065\/apem.2346072.036.","DOI":"10.6065\/apem.2346072.036"},{"key":"2024090408434236883_j_jpem-2024-0283_ref_030","doi-asserted-by":"crossref","unstructured":"Choi, R, Kwon, A, Oh, Y, Lee, SG, Lee, EH. Time points for gonadotropin-releasing hormone stimulation test results in Korean children. 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