{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,6]],"date-time":"2025-07-06T17:03:06Z","timestamp":1751821386177,"version":"3.37.0"},"reference-count":21,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_jpem-2024-0422_abs_001\">\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Limited data are available on the hormonal response of children to venepuncture or intravenous cannulation (IVC). Catecholamines (epinephrine (E)\u00a0and norepinephrine (NE)) have been traditionally recognized as stress hormones. Copeptin, the carboxyl-terminus of the arginine vasopressin (AVP) precursor peptide, is also a known marker for stressful stimuli, including myocardial infarction, critical illness, and sepsis. We aimed to measure the above stress markers in response to IVC in the pediatric population.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpem-2024-0422_abs_002\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We measured plasma E, NE and serum copeptin concentrations in 100 children aged 5\u201317 years undergoing endocrine testing. Labs were drawn 1\u20133\u202fmin (min) after placement of IV cannula (baseline or 0\u202fmin) and then re-measured 20\u202fmin later (+20\u202fmin) while subjects rested in a quiet room.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpem-2024-0422_abs_003\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Between 0 and 20\u202fmin, the median (IQR) NE (n=99) changed from 349 (244, 482) pg\/mL to 253 (184, 348) pg\/mL (p&lt;0.001); E (n=54) changed from 57 (43, 116) pg\/mL to 57 (38, 96) pg\/mL (p=0.024); Copeptin changed from 9.4 (6.3, 15.2) pmol\/L to 9 (5, 13) pmol\/L (p&lt;0.001). The mean decrease (delta) was 106\u202fpg\/mL for NE (28\u202f%, p&lt;0.001), 16\u202fpg\/mL for E (18\u202f%, p=0.042) and 2.7\u202fpmol\/L for copeptin (17\u202f%, p=0.012). There was no correlation between the decrease (expressed as a percentage) in NE vs. E, E vs. copeptin, and NE vs. copeptin.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpem-2024-0422_abs_004\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our data suggest that the stress of IVC induces a rapid increase in NE, E, as previously described, as well as copeptin levels. The copeptin decrement, concordant with the catecholamine trend in the minutes after IVC, supports this peptide (and AVP) as a rapid response marker of stress, and has unclear practical implications for copeptin measurements in evaluating fluid and sodium metabolism disorders in children.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/jpem-2024-0422","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T09:50:31Z","timestamp":1734429031000},"page":"110-115","source":"Crossref","is-referenced-by-count":1,"title":["The effect of phlebotomy and placement of an intravenous catheter on plasma catecholamine and serum copeptin concentrations"],"prefix":"10.1515","volume":"38","author":[{"given":"Shruti","family":"Sastry","sequence":"first","affiliation":[{"name":"Division of Pediatric Endocrinology , UPMC Children\u2019s Hospital of Pittsburgh , Pittsburgh , PA , USA"},{"name":"Department of Pediatrics , University of Pittsburgh , Pittsburgh , PA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emir","family":"Tas","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology , UPMC Children\u2019s Hospital of Pittsburgh , Pittsburgh , PA , USA"},{"name":"Department of Pediatrics , University of Pittsburgh , Pittsburgh , PA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Domenic","family":"Filingeri","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology , UPMC Children\u2019s Hospital of Pittsburgh , Pittsburgh , PA , USA"},{"name":"Division of Endocrinology, Diabetes and Metabolism, Diabetes and Beta Cell Biology Center , University of Pittsburgh , Pittsburgh , PA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"McCann","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology , UPMC Children\u2019s Hospital of Pittsburgh , Pittsburgh , PA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahul","family":"Amruthapuri","sequence":"additional","affiliation":[{"name":"Department of Pharmacology , University of Pittsburgh , Pittsburgh , PA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"McPhaul","sequence":"additional","affiliation":[{"name":"Quest Diagnostics Nichols Institute , San Juan Capistrano , CA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi","family":"Garibaldi","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology , UPMC Children\u2019s Hospital of Pittsburgh , Pittsburgh , PA , USA"},{"name":"Department of Pediatrics , University of Pittsburgh , Pittsburgh , PA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"2025020411462945542_j_jpem-2024-0422_ref_001","doi-asserted-by":"crossref","unstructured":"Kennedy, RM, Luhmann, J, Zempsky, WT. Clinical implications of unmanaged needle-insertion pain and distress in children. Pediatrics 2008;122:S130\u20133. https:\/\/doi.org\/10.1542\/peds.2008-1055e.","DOI":"10.1542\/peds.2008-1055e"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_002","doi-asserted-by":"crossref","unstructured":"Martino, M, Arnaldi, G. Copeptin and stress. Endocrines 2021;2:384\u2013404. https:\/\/doi.org\/10.3390\/endocrines2040035.","DOI":"10.3390\/endocrines2040035"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_003","unstructured":"Robinson AG. The posterior pituitary (Neurohypophysis). In: Gardner, DG, Shoback, D, editors. Greenspan\u2019s basic & clinical endocrinology, 10th ed. New York, NY: McGraw-Hill Education; 2017:121\u201336 pp."},{"key":"2025020411462945542_j_jpem-2024-0422_ref_004","doi-asserted-by":"crossref","unstructured":"Mavani, GP, DeVita, MV, Michelis, MF. A review of the nonpressor and nonantidiuretic actions of the hormone vasopressin. Front Med (Lausanne) 2015;2:19. https:\/\/doi.org\/10.3389\/fmed.2015.00019.","DOI":"10.3389\/fmed.2015.00019"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_005","doi-asserted-by":"crossref","unstructured":"Kluge, M, Riedl, S, Erhart-Hofmann, B, Hartmann, J, Waldhauser, F. Improved extraction procedure and RIA for determination of arginine8-vasopressin in plasma: role of premeasurement sample treatment and reference values in children. Clin Chem 1999;45:98\u2013103. https:\/\/doi.org\/10.1093\/clinchem\/45.1.98.","DOI":"10.1093\/clinchem\/45.1.98"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_006","doi-asserted-by":"crossref","unstructured":"Morgenthaler, NG, Struck, J, Jochberger, S, Dunser, MW. Copeptin: clinical use of a new biomarker. Trends Endocrinol Metab 2008;19:43\u20139. https:\/\/doi.org\/10.1016\/j.tem.2007.11.001.","DOI":"10.1016\/j.tem.2007.11.001"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_007","doi-asserted-by":"crossref","unstructured":"Jalleh, R, Torpy, DJ. The emerging role of copeptin. Clin Biochem Rev 2021;42:17\u201325. https:\/\/doi.org\/10.33176\/AACB-20-00001.","DOI":"10.33176\/AACB-20-00001"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_008","doi-asserted-by":"crossref","unstructured":"Sastry, S, March, CA, McPhaul, MJ, Garibaldi, LR. Very elevated serum copeptin concentrations occur in a subset of healthy children in the minutes after phlebotomy. J Pediatr Endocrinol Metab 2024;37:8\u201314. https:\/\/doi.org\/10.1515\/jpem-2023-0390.","DOI":"10.1515\/jpem-2023-0390"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_009","doi-asserted-by":"crossref","unstructured":"Tuli, G, Munarin, J, Tessaris, D, Einaudi, S, Matarazzo, P, de Sanctis, L. Distribution of plasma copeptin levels and influence of obesity in children and adolescents. Eur J Pediatr 2021;180:119\u201326. https:\/\/doi.org\/10.1007\/s00431-020-03777-3.","DOI":"10.1007\/s00431-020-03777-3"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_013","doi-asserted-by":"crossref","unstructured":"Eijkelenkamp, K, van Geel, EH, Kerstens, MN, van Faassen, M, Kema, IP, Links, TP, et al.. Blood sampling for metanephrines comparing venipuncture vs. indwelling intravenous cannula in healthy subjects. Clin Chem Lab Med 2020;58:1681\u20136. https:\/\/doi.org\/10.1515\/cclm-2020-0022.","DOI":"10.1515\/cclm-2020-0022"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_010","doi-asserted-by":"crossref","unstructured":"Eichler, I, Eichler, HG, Rotter, M, Kyrle, PA, Gasic, S, Korn, A. Plasma concentrations of free and sulfoconjugated dopamine, epinephrine, and norepinephrine in healthy infants and children. Klin Wochenschr 1989;67:672\u20135. https:\/\/doi.org\/10.1007\/bf01718028.","DOI":"10.1007\/BF01718028"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_011","unstructured":"Netter, P. Psychological aspects of catecholamine response patterns to pain and mental stress in essential hypertensive patients and controls. J Clin Hypertens 1987;3:727\u201342."},{"key":"2025020411462945542_j_jpem-2024-0422_ref_012","doi-asserted-by":"crossref","unstructured":"Chortis, V, Bancos, I, Crowley, RK, Arlt, W. Supine or sitting plasma metanephrine screening? A unifying solution for patients and doctors. Clin Endocrinol (Oxf) 2015;82:776\u20137. https:\/\/doi.org\/10.1111\/cen.12659.","DOI":"10.1111\/cen.12659"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_014","doi-asserted-by":"crossref","unstructured":"Darr, R, Pamporaki, C, Peitzsch, M, Miehle, K, Prejbisz, A, Peczkowska, M, et al.. Biochemical diagnosis of phaeochromocytoma using plasma-free normetanephrine, metanephrine and methoxytyramine: importance of supine sampling under fasting conditions. Clin Endocrinol (Oxf) 2014;80:478\u201386. https:\/\/doi.org\/10.1111\/cen.12327.","DOI":"10.1111\/cen.12327"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_015","doi-asserted-by":"crossref","unstructured":"Pommer, G, Pamporaki, C, Peitzsch, M, Remde, H, Deutschbein, T, Nolting, S, et al.. Preanalytical considerations and outpatient versus inpatient tests of plasma metanephrines to diagnose pheochromocytoma. J Clin Endocrinol Metab 2022;107:e3689\u2013e98. https:\/\/doi.org\/10.1210\/clinem\/dgac390.","DOI":"10.1210\/clinem\/dgac390"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_016","doi-asserted-by":"crossref","unstructured":"FitzGerald, GA, Hossmann, V, Hamilton, CA, Reid, JL, Davies, DS, Dollery, CT. Interindividual variation in kinetics of infused epinephrine. Clin Pharmacol Ther 1979;26:669\u201375. https:\/\/doi.org\/10.1002\/cpt1979266669.","DOI":"10.1002\/cpt1979266669"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_017","doi-asserted-by":"crossref","unstructured":"Schofl, C, Becker, C, Prank, K, von zur Muhlen, A, Brabant, G. Twenty-four-hour rhythms of plasma catecholamines and their relation to cardiovascular parameters in healthy young men. Eur J Endocrinol 1997;137:675\u201383. https:\/\/doi.org\/10.1530\/eje.0.1370675.","DOI":"10.1530\/eje.0.1370675"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_018","doi-asserted-by":"crossref","unstructured":"Fenske, WK, Schnyder, I, Koch, G, Walti, C, Pfister, M, Kopp, P, et al.. Release and decay kinetics of copeptin vs AVP in response to osmotic alterations in healthy volunteers. J Clin Endocrinol Metab 2018;103:505\u201313. https:\/\/doi.org\/10.1210\/jc.2017-01891.","DOI":"10.1210\/jc.2017-01891"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_019","doi-asserted-by":"crossref","unstructured":"Bonnet, L, Marquant, E, Fromonot, J, Hamouda, I, Berbis, J, Godefroy, A, et al.. Copeptin assays in children for the differential diagnosis of polyuria-polydipsia syndrome and reference levels in hospitalized children. Clin Endocrinol (Oxf) 2022;96:47\u201353. https:\/\/doi.org\/10.1111\/cen.14620.","DOI":"10.1111\/cen.14620"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_020","doi-asserted-by":"crossref","unstructured":"Cozzi, G, Valerio, P, Kennedy, R. A narrative review with practical advice on how to decrease pain and distress during venepuncture and peripheral intravenous cannulation. Acta Paediatr 2021;110:423\u201332. https:\/\/doi.org\/10.1111\/apa.15526.","DOI":"10.1111\/apa.15526"},{"key":"2025020411462945542_j_jpem-2024-0422_ref_021","doi-asserted-by":"crossref","unstructured":"Havekes, B, van der Klaauw, AA, Weiss, MM, Jansen, JC, van der Mey, AG, Vriends, AH, et al.. Pheochromocytomas and extra-adrenal paragangliomas detected by screening in patients with SDHD-associated head-and-neck paragangliomas. Endocr Relat Cancer 2009;16:527\u201336. https:\/\/doi.org\/10.1677\/erc-09-0024.","DOI":"10.1677\/ERC-09-0024"}],"container-title":["Journal of Pediatric Endocrinology and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpem-2024-0422\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpem-2024-0422\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T11:47:04Z","timestamp":1738669624000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpem-2024-0422\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,18]]},"references-count":21,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,12,17]]},"published-print":{"date-parts":[[2025,2,25]]}},"alternative-id":["10.1515\/jpem-2024-0422"],"URL":"https:\/\/doi.org\/10.1515\/jpem-2024-0422","relation":{},"ISSN":["0334-018X","2191-0251"],"issn-type":[{"type":"print","value":"0334-018X"},{"type":"electronic","value":"2191-0251"}],"subject":[],"published":{"date-parts":[[2024,12,18]]}}}