{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T18:20:07Z","timestamp":1772043607044,"version":"3.50.1"},"reference-count":27,"publisher":"Walter de Gruyter GmbH","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Growth hormone deficiency (GHD) diagnosis still lacks a gold standard or ideal diagnostic marker. Unlike other epigenetic mechanisms, non-coding RNAs regulate post-transcriptional levels. The information on non-coding RNAs in the field of GHD is limited. Therefore, this study aimed to explore the role of hsa_circ_0002473 as a competitive endogenous RNA for has-miR-4645-3p in attenuating the inhibitory effect of has-miR-4645-3p on SSTR2. In this study, we screened three significantly expressed circular RNAs (circRNAs) in five children with GHD, and selected the highest expressed hsa_circ_0002473 as the study object, and screened has-miR-4645-3p, which is the most likely to bind to hsa_circ_0002473, according to the microRNA (miRNA)-circRNA regulatory network, to study the role and mechanism of has-miR-4645-3p as a competitive endogenous RNA of has-miR-4645-3p on GH3 cells. Somatostatin receptor 2 (SSTR2) inhibits GH3 cell proliferation, and miRNA binding to SSTR2 inhibits the latter expression. Both bioinformatics and dual-luciferase reporter analyses showed targeting relationships between hsa_circ_0002473 and has-miR-4645-3p and between has-miR-4645-3p and SSTR2. We constructed the hsa_circ_0002473\/has-miR-4645-3p axis and transfected it into GH3 cells and found that overexpression of hsa_circ_0002473 inhibited the proliferation and growth hormone (GH) secretion of GH3 cells, and that hsa-miR-4645-3p promoted the proliferation and GH secretion of GH3 cells by targeting SSTR2. Co-culture revealed that the inhibitory effect of hsa_circ_0002473 was reversed by has-miR-4645-3p. In conclusion, our findings suggest that hsa_circ_0002473 can act as a competitive endogenous RNA for has-miR-4645-3p to regulate GH3 cell proliferation and secretion by targeting SSTR2.<\/jats:p>","DOI":"10.1515\/jpem-2024-0449","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T12:50:44Z","timestamp":1728910244000},"page":"1054-1065","source":"Crossref","is-referenced-by-count":0,"title":["Hsa_circ_0002473 inhibits GH3 cell proliferation and GH secretion as a competitive endogenous RNA for has-miR-4645-3p"],"prefix":"10.1515","volume":"37","author":[{"given":"Kaiyu","family":"Pan","sequence":"first","affiliation":[{"name":"Department of Pediatrics , Xiaoshan Affiliated Hospital of Wenzhou Medical University , Hangzhou , Zhejiang , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoguang","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics , Xiaoshan District Hospital of Traditional Chinese Medicine and Orthopedics , Hangzhou , Zhejiang , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohong","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics , Xiaoshan Affiliated Hospital of Wenzhou Medical University , Hangzhou , Zhejiang , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhua","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics , Xiaoshan Affiliated Hospital of Wenzhou Medical University , Hangzhou , Zhejiang , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengyue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology , National Clinical Research Center for Child Health, The Children\u2019s Hospital, Zhejiang University School of Medicine , Hangzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"2026022517525481232_j_jpem-2024-0449_ref_001","doi-asserted-by":"crossref","unstructured":"Maghnie, M, Orso, M, Polistena, B, Cappa, M, Pozzobon, G, d\u2019Angela, D, et al.. 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