{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:43:14Z","timestamp":1791938594596,"version":"4.3.4"},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Perinatal Medicine"],"published-print":{"date-parts":[[2013,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>\n                  <jats:bold>Aim:<\/jats:bold> Tissue culture studies indicate that bacterial products stimulate the production of proinflammatory cytokines by reproductive tissues. However, most of these studies have been performed under room air conditions, supplemented with 5% CO<jats:sub>2<\/jats:sub>. In this study, we tested whether O<jats:sub>2<\/jats:sub> tension affects bacteria-stimulated cytokine production by extra-placental fetal membranes.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methods:<\/jats:bold> Cultures of full-thickness membranes, isolated choriodecidua, and isolated amnion were exposed to bacteria and incubated under 21% (room air) or 5% O<jats:sub>2<\/jats:sub> for 18 h. Cytokine concentrations in conditioned medium was quantified by immunoassay.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results:<\/jats:bold> Culture under 5% O<jats:sub>2<\/jats:sub> increased production of interleukin (IL)-1\u03b2 and tumor necrosis factor (TNF)-\u03b1, but reduced IL-10 and IL-6 production by full membranes. Isolated choriodecidua responded to 5% O<jats:sub>2<\/jats:sub> with increased IL-1\u03b2 production and reduced IL-6 production, but had no effect on TNF-\u03b1 and IL-10 production was not detected. No effect of O<jats:sub>2<\/jats:sub> tension on IL-1\u03b2 or IL-6 production by isolated amnion was detected, however, <jats:italic>Escherichia coli<\/jats:italic>-stimulated IL-10, TNF-\u03b1 and IL-8 production was enhanced by culture under 5% O<jats:sub>2<\/jats:sub>.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions:<\/jats:bold> Increased oxygen tension reduces the pro-inflammatory responsiveness of cell cultures to <jats:italic>E. coli<\/jats:italic> and promotes an anti-inflammatory cytokine profile. Differential effects of O<jats:sub>2<\/jats:sub> tension on choriodecidua and amnion suggests a network of paracrine factors that regulate cytokine levels in response to changes in O<jats:sub>2<\/jats:sub> tension.<\/jats:p>","DOI":"10.1515\/jpm-2012-0294","type":"journal-article","created":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T20:33:23Z","timestamp":1369946003000},"page":"595-603","source":"Crossref","is-referenced-by-count":5,"title":["Effect of oxygen tension on bacteria-stimulated cytokine production by fetal membranes"],"prefix":"10.1515","volume":"41","author":[{"given":"Natalia G.","family":"Klimova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazeeh","family":"Hanna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgan R.","family":"Peltier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2013,5,16]]},"container-title":["jpme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpm-2012-0294\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpm-2012-0294\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T03:23:58Z","timestamp":1678764238000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpm-2012-0294\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,16]]},"references-count":0,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2013,5,16]]},"published-print":{"date-parts":[[2013,9,1]]}},"alternative-id":["10.1515\/jpm-2012-0294"],"URL":"https:\/\/doi.org\/10.1515\/jpm-2012-0294","relation":{},"ISSN":["1619-3997","0300-5577"],"issn-type":[{"value":"1619-3997","type":"electronic"},{"value":"0300-5577","type":"print"}],"subject":[],"published":{"date-parts":[[2013,5,16]]}}}