{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:11:04Z","timestamp":1767856264138,"version":"3.49.0"},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,1]]},"abstract":"Abstract<\/jats:title>\n \n Aims:<\/jats:bold> Peroxisome proliferator-activated receptor-gamma (PPAR\u03b3) plays an important role in insulin metabolism, trophoblast differentiation and anti-inflammatory circuits. The aim of this study was to investigate the expression of PPAR\u03b3 in the placenta of patients with gestational diabetes mellitus (GDM) and the regulation of PPAR\u03b3 by its agonists in trophoblast tumour cells BeWo in vitro<\/jats:italic>.<\/jats:p>\n \n Methods<\/jats:bold>: PPAR\u03b3 expression in a total of 80 placentas (40 GDM\/40 controls) was analysed by immunohistochemistry using the semi-quantitative immunoreactive score. Furthermore, a quantitative reverse transcription-polymerase chain reaction (PCR) was performed to determine the PPAR\u03b3 mRNA-expression in both groups. We used a fused and a non-fused BeWo cell culture model for the stimulation with arachidonic acid and 15-Deoxy-\u039412,14<\/jats:sup>-prostaglandin J2<\/jats:sub> (15d-PGJ2). Afterwards PPAR\u03b3 mRNA-expression was analysed by quantitative real-time PCR (RT-PCR) (TaqMan).<\/jats:p>\n \n Results:<\/jats:bold> Using immunohistochemistry we identified a decreased expression of PPAR\u03b3 in the syncytiotrophoblast and the extravillous trophoblast of GDM placentas compared to normal controls. Furthermore, PPAR\u03b3 mRNA-expression was reduced in GDM placentas. Stimulation of BeWo cells with arachidonic acid and 15d-PGJ2 caused a downregulation of PPAR\u03b3 expression.<\/jats:p>\n \n Conclusion:<\/jats:bold> As PPAR\u03b3 is down regulated by arachidonic acid and 15d-PGJ2, the reduced PPAR\u03b3 expression in GDM placentas may be due to an altered concentration of fatty acid derivates.<\/jats:p>","DOI":"10.1515\/jpm-2013-0039","type":"journal-article","created":{"date-parts":[[2013,11,4]],"date-time":"2013-11-04T20:30:36Z","timestamp":1383597036000},"page":"179-187","source":"Crossref","is-referenced-by-count":20,"title":["Peroxisome proliferator-activated receptor-gamma (PPAR\u03b3) is down regulated in trophoblast cells of gestational diabetes mellitus (GDM) and in trophoblast tumour cells BeWo in vitro<\/i> after stimulation with PPAR\u03b3 agonists"],"prefix":"10.1515","volume":"42","author":[{"given":"Julia","family":"Knabl","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-University Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"H\u00fcttenbrenner","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-University Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Hutter","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-University Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"G\u00fcnthner-Biller","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-University Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Vrekoussis","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-University Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrin","family":"Karl","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-University Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Friese","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franz","family":"Kainer","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-University Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udo","family":"Jeschke","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Campus Innenstadt, Ludwig-Maximilians-University Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2013,11,5]]},"container-title":["Journal of Perinatal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpm-2013-0039\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpm-2013-0039\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T03:25:32Z","timestamp":1678764332000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpm-2013-0039\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,5]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2013,11,21]]},"published-print":{"date-parts":[[2014,3,1]]}},"alternative-id":["10.1515\/jpm-2013-0039"],"URL":"https:\/\/doi.org\/10.1515\/jpm-2013-0039","relation":{},"ISSN":["1619-3997","0300-5577"],"issn-type":[{"value":"1619-3997","type":"electronic"},{"value":"0300-5577","type":"print"}],"subject":[],"published":{"date-parts":[[2013,11,5]]}}}